CCM-300
Chlorophyll Content meter for small leaves and
difficult samples
Operation manual
603–883-4400 www.optisci.com
Page 1: ...CCM 300 Chlorophyll Content meter for small leaves and difficult samples Operation manual 603 883 4400 www optisci com ...
Page 2: ...tion effect 9 Re absorption of the emitted chlorophyll fluorescence by the leaf chlorophylls 10 Temperature 10 Fluorescence emission signal strength 10 References 12 Setup Mode 13 Measurement Mode 14 For measurements with the leaf clip 15 For measurements without the leaf clip 16 Data Management File Utilities 17 Erasing a file on the instrument 17 Viewing a file on the instrument 18 Diagnostics M...
Page 3: ...may be used Two sets of NiMH batteries and a NiMH charger are included with the unit The unit will typically run for 8 10 hrs on a freshly charged set of NiMH batteries Ambient temperature and battery charge will affect runtime The unit has a 320x480 pixel display with touch sensitive screen While finger touch may be used successfully for most functions A stylus is provided for easier use of some ...
Page 4: ... try reseating the fiber connection Fibers should seat securely The sample end of the fiber can be used with the sample clip if thin flat samples such as leaf tissue are to be tested The set screw has a nylon tip It has been set at the factory for a firm press fit If the fiber is loose or does not fit correctly the set screw may be adjusted The fiber optic can be used without the leaf clip See the...
Page 5: ...sing the on screen keyboard Screens having a green character in the upper right corner include help information that may be viewed by tapping it The help box will display information about the current screen An option to capture a snapshot of the current screen is available in the help box PScrn This can be useful when diagnosing a system problem When this feature is used a box will appear asking ...
Page 6: ...ssion of red and infrared light The surface must be relatively flat and uniform Variable fluorescence or the Kautsky effect caused by the red wavelength of absorption instruments limits the repeatability of measurements Selection of the measuring area on smaller leaves can cause measurement variations due to leaf structure variation Veins and midribs can cause significant variation The two wavelen...
Page 7: ... testing in a wide range of chlorophyll content 41 mg m 2 to 675 mg m 2 The advantage of this ratio is that it works exceptionally well even above chlorophyll content levels of 200 mg m 2 By raising the lower fluorescent emission measuring range away from chlorophyll absorption band near 680 nm or 685 nm to 700 nm the amount of fluorescence light that is reabsorbed and emitted again as chlorophyll...
Page 8: ...osynthesis research as well as in agriculture horticulture and forestry The variables Fluorescence emission response to chlorophyll content As chlorophyll content levels increase the intensity of the selected far red fluorescence emission at 735 nm increases and then decline slightly At the shorter fluorescence emission wavelength 700 nm the value increases and then it declines dramatically The Gi...
Page 9: ... seconds for 20 minutes With the CCM 300 using the F735 nm F700 nm ratio the Kautsky induction effect is negligible Negligible Kautsky induction effects with the CCM 300 9 Unlike the CCM 300 F735 F700 fluorescence ratio other ratios such as F 690 F735 have shown Kautsky induction effects It has been found that the fluorescence emission ratio 690 735 is affected by the onset of photosynthesis and e...
Page 10: ...9 Temperature Buschmann reports that the ratio of F690 F735 a similar ratio but not F735 F700 can drop by up to 25 as temperatures drop from 23o C to 4o C This is thought to be influenced by state transitions and PSI fluorescence This shows that the instrument can be used for plant stress measurement but it also shows that for direct chlorophyll content comparison measurement applications temperat...
Page 11: ...ample was measured 30 times at the same location Samples with greater variation require an average of multiple measurements for the best results For this reason measuring options are included to provide averaging from 2 to 30 measurements or averaging with outlier removal beyond 2 sigma or median determination 11 ...
Page 12: ...he aperture does not need to be filled small samples such as turf grasses rice and Arabidopsis can be measured and because the method uses ratio fluorescence difficult shapes can be measured such as conifer leaves References Gitelson A A Buschmann C Lichtenthaler H K 1999 The Chlorophyll Fluorescence Ratio F735 F700 as an Accurate Measure of Chlorophyll Content in Plants Remote Sens Enviro 69 296 ...
Page 13: ...able from 5 minutes to 20 minutes The horn icon contains the controls for the beeper and auto off feature Tapping the speaker icon will toggle the beep tone on off The Calibrate Screen button will adjust the touch panel The screen is pre calibrated A calibration is only needed if a key location and its active press area do not coincide This may be most apparent when entering in file names using th...
Page 14: ...racters The default extension CSV comma separated value is used with the chosen name This name will become the default name until it is changed again 2 Set the averaging function to the desired method and quantity In the Averaging box tap on this box to make changes A new box will appear with arrow and quantities Tap the arrows to scroll through the options The options include off for single point...
Page 15: ...he end of the fiber optic completely into the leaf clip and tighten the Allen screw so that it is snug Place the sample in such a way that it is located directly under the middle of the end of the fiber optic The fiber optic aperture does not have to be completely filled by the sample Tap the Measure box It takes five seconds to make a measurement The Ratio and direct chlorophyll content values ar...
Page 16: ...s close to perpendicular to the sample surface This will help ensure repeatable results Direct contact with the sample surface is recommended for repeatable results Dark adapting or light adapting do not appreciably affect measurements See the results on page 9 for more details Here again the signal bar will help determine the number of measurements required for best results The signal window on t...
Page 17: ...Utilities Erasing a file on the instrument Data saved on the unit may be erased or viewed from this menu Data may be moved between the unit and a host PC in the menu also The unit will appear on the pc as a Removable Memory device if a USB cable is connected now 17 ...
Page 18: ...ll display a smaller window to confirm the erase action Once erased a file cannot be restored Viewing a file on the instrument Tapping on View a File will display a list of files stored in the instrument Tap on the file of interest or scroll up and down using the arrow buttons on the right hand side When the file of interest is found tap on the file name and the data in the file may be viewed Deta...
Page 19: ...voltage of less than 2 20V indicates that the charge in the battery is almost used up A freshly charged set should be installed before beginning a new round of measurements A voltage greater than 2 50V is recommended for alkaline batteries The Temp temperature in degrees F and Bias Voltage readings display detector environment values The middle box shows preprocessed signal raw data info and a gra...
Page 20: ...with any other mobile storage device Attach USB cable to PC File Utilities Remote access screen is displayed Note Do not disconnect the instrument without first using the eject function on the PC Failure to use the eject function may result in the loss of data and possible corruption of the CCM 300 file system To use the eject function select the device from Windows Explorer right mouse click and ...
Page 21: ... diameter stainless steel tip Calibration Solid state sensors and light sources provide no significant variation over the life of the instrument The measurement represents a ratio of fluorescence at two different emission wavelengths The research shows that this ratio provides a precise measurement of Chlorophyll content from 41mg m 2 to 675 mg m 2 using Beech Elm or Wild Vine Calibration may be c...
Page 22: ...to host PC Power Supply 2 AA batteries Can use either NiMH or alkaline batteries A charger is supplied Operating Life Typically 8 to 10 hrs with freshly charged batteries Operating Temperature 33 to 120 degrees F 1 to 48 degrees C Dimensions 4 7 x 3 5 x 1 2 12cm x 9cm x 3cm Weight 0 60 lbs 275g ...
