Last updated on Mar 2022
4.2 Software Initiation
Wait for complete initialization of microscope and Windows after logging in with your TLL
username and password.
Double click on:
(A) The HW configuration screen will appear:
Select Enable XY stage control
Click on
OK
and wait for complete initialization. Do
not move anything on the microscope during the
initialization process.
(B) Execution of IX3-SSU clean-up will pop up:
If you are the first user of the week:
Rotate the
turret so that either the 1.25× or 10× objective is in
place. Click on
YES
and wait for complete
initialization. The cleaning process uses ultrasonic vibrations to dislodge dust that has
accumulated on the stage mechanism. This adds time to the initialization process. Do not
place anything on the stage mechanism until the initialization process is complete.
If you are not the first user of the week:
Click on
NO
and wait for complete initialization. Do
not move anything on the microscope during the initialization process.
On the FV31S-SW software, press on the ocular tab to
observe the specimen using either bright field/DIC or
fluorescence mode.
•
For Brightfield imaging, select
Ocular>Ocular>DIA.
Select Cube turret #1:
Empty
Under DIA,
ON
both TD shutter and Trans lamp.
Increase intensity of Trans lamp for specimen
viewing.
•
For Fluorescence imaging, select
Ocular > Ocular >
EPI.
Select Cube turret #1
: DAPI/FITC/TRITC
Select the appropriate EPI ND Filter to control the
fluorescence intensity.
Then EPI shutter:
Open
※
Keep it
Closed
until you are actively viewing your sample.