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BX53/51/41-P

In polarized light microscopy ultimate performance is not obtainable unless optical adjustments are made correctly.
Always performed the following adjustments before observation. Remove the quarter wave plate and sensitive tint plate
from the light path.

When Using the Intermediate Attachment for orthoscopic observation
(U-OPA) (Figs. 19 & 20)

Make sure the 10X objective is inserted into the primary hole on
the centerable revolving nosepiece.

1. Fully insert the alignment polarizer @, provided with the U-OPA into

the test plate adapter (U-TAD). Tighten its clamping knob. (Fig. 19)

2. Remove the condenser.
3. Insert the rotatable analyzer (U-AN360P-2) ² into the analyzer slot on

the intermediate attachment. Engage the analyzer, loosen the clamping
screw and set the analyzer’s rotation dial ³ to 0°. (Fig. 19)

POLARIZED LIGHT OBSERVATION

5-1  Adjustments Before Observation

Fig. 19

1

    Adjusting the Optical Axis

@

²

³

CAUTION

Summary of Contents for BX53-P

Page 1: ...e yourself fully with the use of your microscope we recommend that you read this manual together with the instruction manual for the BX53 51 41 microscope thoroughly before use Retain this instruction...

Page 2: ......

Page 3: ...t 15 5 2 Orthoscopic Observation 25 5 3 Conoscopic Observation 26 1 Adjusting the Optical Axis 16 21 2 Adjusting for Extinction 22 3 Adjusting the Eyepiece Cross Lines 23 24 5 1 Adjustments Before Obs...

Page 4: ...the microscope check the regulations and rules of your local government and be sure to observe them If the microscope is used in a manner not specified by this manual the safety of the user may be im...

Page 5: ...chment Centerable revolving eyepiece Polarizing rotatable stage or Rotatable stage Observation tube Objective Strain free objective series Lamp housing Components for polarized light observation Strai...

Page 6: ...free objective Mechanical stage U FMP Stage clips Polarizing rotatable stage U SRP Rotatable stage U SRG2 Polarizing intermediate attachment U CPA Observation tube Intermediate attachment for AN360P U...

Page 7: ...the stage bracket with the positioning pin on the stage aligned with the groove on the front of the stage bracket then loosely tighten the centering knobs Fig 1 Mounting Stage Clips and Mechanical Sta...

Page 8: ...4 Position the condenser with the scale markings in front insert it into the substage fork as far as it will go Align the positioning pin at the back of the condenser with the groove in the substage f...

Page 9: ...a clock wise direction from the primary hole 4 Mounting the Revolving Nosepiece U P4RE Fig 5 1 Turn the coarse adjustment knob to lower the stage all the way 2 Using the Allen screwdriver loosen the...

Page 10: ...n of this intermediate tube can be adjusted later At this point place the tube so the nameplate is toward the rear 1 Using the Allen screwdriver loosen the observation tube clamping screw on the micro...

Page 11: ...n the observation tube by tightening the clamping screw 7 Mounting the Eyepieces Insert the eyepiece with cross lines into the right hand eyepiece sleeve Make sure the eyepiece is inserted so that the...

Page 12: ...he test plate adapter U TAD Position the fixed analyzer so that the positioning pin on the fixed analyzer fits into the groove The fixed analyzer will be held in place by a magnet 1 Loosen the test pl...

Page 13: ...ro duced on the following pages Bertrand lens knob CONTROLS Slider clamping knob Mechanical stage Vernier scale Aperture iris diaphragm lever Polarizer rotation ring Bertrand lens focusing knob Analyz...

Page 14: ...ssible Stopper knob Rotation dial Clamping knob Test Plate Adapter U TAD Test plate clamping knob Hole for the fixed analyzer U ANT Slot for compensator plate Sensitive Tint Plate U TP530 Quarter Wave...

Page 15: ...e center and clamp the specimen with the stage clips When Using the Mechanical Stage U FMP Fig 12 Open the spring loaded finger and place the specimen on the stage Applicable slide glass is for inorga...

Page 16: ...ever located at the right side of the stage is moved toward the observer and the stage is moved from this position to the first click stop position the specimen is moved 45 to its diagonal position To...

Page 17: ...roscope See page 4 2 Using the Allen screwdriver loosen the substage bracket clamping screw and remove the substage assembly Fig 15 3 Turn the coarse adjustment knob and raise the focusing block to wh...

Page 18: ...lyzer Fig 18 The analyzer is engaged when pushed in to the second click stop position To disengaged the analyzer and to place the empty hole in the light path pull the analyzer out to the first click...

Page 19: ...Make sure the 10X objective is inserted into the primary hole on the centerable revolving nosepiece 1 Fully insert the alignment polarizer provided with the U OPA into the test plate adapter U TAD Tig...

Page 20: ...Place the specimen 2 Focus on the specimen and look for an easily recognizable detail in the field Move this detail in the center of the eyepiece cross lines 3 When the stage is rotated the detail mov...

Page 21: ...1 Pull out the Bertrand lens knob to the OUT position Fig 23 2 Center the condenser For details see instructions of each microscope 3 Follow steps 9 and 10 in When Using the Intermediate Attachment f...

Page 22: ...escribed in the following paragraph When Using Intermediate Attachment for conoscopic and orthoscopic observation U CPA Photomicrography 1 Using the tip of a mechanical pencial etc push out in the dir...

Page 23: ...6 7 Insert the two provided centering wrenches into the centering holes for the primary hole with the 20X or 10X objective on the nosepiece 8 Manipulate the centering wrenches to bring the bright cent...

Page 24: ...ntration this will move the specimen detail in the opposite direc tion away from the cross line center 4 Moving the specimen only move a new specimen detail into the cross line center Repeat steps 3 a...

Page 25: ...le analyzer into the light path and set vibration direction scale at the 0 position Clamp with the clamping knob Fig 30 3 Set the polarizer scale at the 0 position 4 Loosen the polarizer clamping knob...

Page 26: ...Orientation plate U PJ Single module name Remove the test plate adapter and the compensator from the light path 1 Swing out the top lens of the condenser 2 Place the orientation plate on the stage Re...

Page 27: ...rving rotate the stage to locate the position where the orientation plate is darkest At this point clamp the stage 5 Remove the analyzer from the light path Set to brightfield and slightly loosen the...

Page 28: ...the Bertrand lens from the light path 2 Insert the analyzer and perform observation When the top lens is swung out the aperture iris diaphragm and field iris diaphragm will not function nor mally If t...

Page 29: ...Slide the focusing knob on the U CPA to focus on the conoscopic image When the intermediate attachment conoscopic and orthoscopic obser vation U CPA is not used conoscopic observation is possible by...

Page 30: ...t for rotatable analyzer U AN360P 2 3 Revolving nosepiece U P4RE 360 dial rotation Minimum scale reading 0 1 Vernier scale Type Centerable quadruple Attachable compensators Quarter wave plate U TP137...

Page 31: ...to 50 relative humidity at 40 C 104 F Main supply voltage fluctuations not to exceed 10 of the nominal voltage Installation Overvoltage Category II In accordance with IEC60664 Pollution Degree 2 In ac...

Page 32: ...er glass thickness mm Resolution m Eyepiece WHN10X FN22 UIS2 objective Total mag Depth of focus m Field of view PLN P Plan Achromat for polarized light FN22 ACHN P Achromat for polarized light FN22 Th...

Page 33: ...b Vignetting or uneven field of view Test plate is stopped at an intermediate position Set at click stop position During orthoscopic observation the con denser top lens is placed in the light path or...

Page 34: ...MEMO...

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Page 36: ...rmany 3500 Corporate Parkway Center Valley Pennsylvania 18034 0610 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 31 Gilby Road Mount Waverley VIC 3149 Melbourne Aust...

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