OLS CASY TTC Operator'S Manual Download

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Summary of Contents for CASY TTC

Page 2: ...rical Safety 9 IX Disposal of the Instrument 9 1 BASIC EQUIPMENT 10 2 INTRODUCTION 11 2 1 Functionality 11 2 2 Measuring Principle 11 2 2 1 Digital Pulse Processing 12 2 2 2 Pulse Area Analysis 13 2 2...

Page 3: ...SY 28 6 1 Switch ON OFF and Login 28 6 2 Dashboard and Software Functions 29 6 2 1 System Settings 30 6 2 2 User Management 31 6 2 3 Emergency Password 31 6 3 Service 32 6 3 1 Service Menu Dry Liquid...

Page 4: ...ty Storage Container 44 7 4 6 Measuring Time Too Short 44 7 4 7 Interrupting the Measurement 44 7 5 Measurement results 45 7 5 1 Opening Measurement results 45 7 5 2 Overlay Mean View 45 7 5 3 Table v...

Page 5: ...KG Karl Ferdinand Braun Str 2 28359 Bremen Phone 0421 27 61 69 0 email info ols bio de Online Support CASY online support is available on www cellcounting de direct online contact manuals literature t...

Page 6: ...EU on the alignment of the legal provisions of the Member States on the electromagnetic equipment for use within certain voltage limits To assess the product with regard to these Council Directives t...

Page 7: ...echniques and have studied the instructions for use of this instrument The CASY is not intended for use in diagnostic procedures VII Conventions Used in this Operator s Guide Symbols In this Operator...

Page 8: ...ling CASYton and CASYclean Waste contaminated with tissue or cells for example waste containers CASYcups pipette tips etc must be disposed according to the recommended rules for handling biohazardous...

Page 9: ...ely defeat the safety features of this instrument If the power cord becomes cracked frayed broken or otherwise damaged replace it immediately with the equivalent part from OLS OMNI Life Science IX Dis...

Page 10: ...rode Protection shield including cable Adapter and USB cable for communication between control and measuring unit Main power supply cable to the measuring unit Binder containing documents including Op...

Page 11: ...correction Determination of the mean degree of cell aggregation The aggregation factor is used to calculate the total number of single cells including cells in cell clusters aggregation correction Via...

Page 12: ...ant flow speed Signal Generation The precision measuring pore is a hole which is cast inside the capillary body The electrolyte filled measuring pore represents a defined electrical resistance constan...

Page 13: ...4 display channels is finally calculated 2 2 3 Measuring Range Dynamics The signal amplifier technology developed specifically for the CASY instrument guarantees excellent measuring range dynamics 1 7...

Page 14: ...or specific applications capillaries with different pore sizes are available covering different measuring ranges In addition to the 150 m capillary the 45 m capillary pore size 45 m and the 60 m capil...

Page 15: ...l current exclusion method Dead and Viable Cursors Viability Mode As the volume of the cell nucleus is significantly smaller than the volume of the entire cell the populations debris dead cells and vi...

Page 16: ...of the representative single cell peak volume to calculate the total number of single cells Cell aggregates thereby are classified into their single cells thus making it possible to determine the sin...

Page 17: ...protection for the highly sensitive measuring electronics The additional use of a protection shield positioned around the capillary during the measurements completely avoids external interference Cons...

Page 18: ...pillary under constant low pressure The progress can be tracked in the inspection window The time required to reach the light barriers depends on the diameter of the measuring capillary and is precise...

Page 19: ...ed or pinched during assembly Then carefully plug in the external electrode without bending it and return it to its original position Step by step instructions for changing the capillary can also be f...

Page 20: ...ol Device The CASY can be controlled by computer notebook or tablet fulfilling the following requirements below It can be ordered separately through OLS OMNI Life Science or your local distributor Ope...

Page 21: ...stic environment it may cause radio interference in which case you may need to take measures to mitigate the interference The electromagnetic environment should be evaluated prior to operation of the...

Page 22: ...ing on for the first time ensure that the mains voltage set on the Voltage Selectors on the rear of the device are consistent with your mains power supply If it is necessary to convert the mains power...

Page 23: ...a Installation location default C Users UserName AppData Roaming Casy b IMPORTANT User must have read and write permissions in installation directory c A desktop icon will be created by default When...

Page 24: ...up Policy Editor gpedit msc In the left pane of Local Group Policy Editor navigate to the location below see screenshot below In the right pane of Edge UI in Local Group Policy Editor double click tap...

Page 25: ...gent CASYclean is available from OMNI Life Science GmbH Co KG The system should be cleaned using only the CASYclean agent specified by OMNI Life Science GmbH Co KG Never use aggressive cleaning fluids...

Page 26: ...the screen Depending on the status of the capillary the software may ask you to remove the capillary from the instrument and sonicate it for 5 min For detailed instructions please see section 3 2 5 3...

Page 27: ...DRY from Service menu for two times 6 Empty the waste container and the two CASYcups 7 Switch off instrument and Control Device now 8 Ensure that the system in adequately protected from dust while it...

Page 28: ...Science installation of the Control Software is required see section 4 3 After start of the CASY Control Software the instrument will perform a self test Hardware pressure system and Memory will be t...

Page 29: ...e Button on Dashboard or Top Menu used to purge the instrument with CASYton from the CASYton storage container Measurement Button on Dashboard or Top Menu initiates a new measurement Undo and Redo Top...

Page 30: ...system It includes setting for languages also see 6 2 2 User Management allows to define Annotation Types or to make settings related to automate Backups Figure 12 Dashboard allowing fast measurement...

Page 31: ...n when finished The first time the new User will try to login he will need to set a password Depending on the security level right some program functions will not be available You can change your Pass...

Page 32: ...t be drained Detailed instructions how to set the system out of operation can be found in section 5 5 The Dry Liquid System command completely drains the liquid system and blows air through it for som...

Page 33: ...ring the final factory check of your device and forms the basis for comparison with all subsequent checks 6 3 4 Enter Service PIN Enter Service PIN allows activating commands of the integrated service...

Page 34: ...an unsuitable location or relating to impurities in the system manifest themselves in a high background To guarantee that the CASY cup is fixed rotate the sample platform so that the recess is no lon...

Page 35: ...aliquot of the cell suspension to be examined normally between 10 l and 200 l Secure the lid and mix the sample by tilting the CASY cup three times carefully avoiding the formation of bubbles or foam...

Page 36: ...irectly from there The CASY System automatically works through all the necessary steps During the measurement a status dialogue is displayed telling you which measuring cycle has just been performed A...

Page 37: ...is finished there are several options to continue working with the result 1 Save the graph 2 Print will generate a report in PDF version Enable printing reports 3 Save Measurement store the measuremen...

Page 38: ...d 45 m capillary 1 cycle with 200 l is recommended Choose between Viability and Free Ranges mode Viability will provide a pair of cursors one to define the dead and one to define the viable cells Free...

Page 39: ...ithout existing Template When the result is displayed it may be necessary to adjust some of the parameters above and repeat the measurement to receive optimal results When the results meets the above...

Page 40: ...or dead and one for the viable cells To identify the size in which the dead cells peak viable cells can be incubated with CASYblue After the sample has been measured and suitable size range and diluti...

Page 41: ...The settings can be stored in Templates and will be automatically used for new measurements with the corresponding template Figure 21 Options for Aggregation correction in Overlay View ON will switch...

Page 42: ...o longer running cleanly and drops of fluid remain suspended on the edge the calculation of your sample concentration may be adversely affected In such cases run a cleaning cycle using CASYclean 7 4 1...

Page 43: ...ling cup To prevent your sample becoming diluted or contaminated replace it with a CASY cup containing freshly filtered CASYton before you run a Purge cycle You will see the Measuring Timeout error me...

Page 44: ...cked In this case contact OMNI Life Science GmbH Co KG 7 4 6 Measuring Time Too Short The error message Measuring Time too short will displayed if time for measurement is to short One reason can be th...

Page 45: ...they can be displayed in an overlay by clicking on Overlay view in the left side bar To be displayed in one overlay the measurements need to be generated using the same measurement parameters If the s...

Page 46: ...e Figure 22 Table View 7 5 4 Importing Measurement results The arrow down button in Measurement results allows to import data into the software Data that can be imported includes measurements in csy F...

Page 47: ...ements were already discussed in previous sections 7 4 Additional information can be found on the website under www cellcounting de If you cannot solve the problem using the answers given in this sect...

Page 48: ...ture and function of the fluid pressure system of the CASY Instrument Depending on the settings of Valve 5 and Valve 3 the pump 1 creates a highor low pressure which is buffered by the pressure vessel...

Page 49: ...2 T 1 6A SI3 T 400mA SI4 T 2 5 A SI5 T 400mA Installation Category II according to IEC 61010 1 Volume Measuring Tube and Pressure System Calibrated opto electronically controlled volume measuring tube...

Page 50: ...the CASYclean agent The surfaces of the CASY System cylinders and sampling chamber should be cleaned regularly using a damp microfiber cloth and 80 ethanol If a service is necessary or requested by t...

Page 51: ...research only Not for use in diagnostic procedures OMNI Life Science GmbH Co KG Karl Ferdinand Braun Stra e 2 28359 Bremen T 49 421 276 16 9 0 F 49 421 276 16 9 19 info ols bio de www ols bio de www...

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