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H301-OLYMPUS-IX3-SVR

User Manual

REV 03

www.oko-lab.com

Pa

ge

2

Index

Components and dimensions................................................................................................... 3

Available Sample Holders ....................................................................................................... 3

Available Lids ..................................................................................................................... 5

Sliding lid .......................................................................................................................... 5

Koehler Lid ........................................................................................................................ 6

Injection Lid ...................................................................................................................... 6

Laser Interlock Lid ............................................................................................................... 7

Insertion of the Sample Feedback Temperature Sensor .................................................................. 7

Insertion of Sample Holder into Chamber ................................................................................... 8

Working with 35 or 60 mm Petri Dish

Spacing Rings and Magnetic Locks ........................................... 9

Working with

1x3’’ and 1x2’’ chamber slides

- magnetic locks ........................................................11

Working with MW Plates - Magnetic locks and Chamber riser ..........................................................12

Connection of the Gas Supply ................................................................................................12

Working with Perfusion ........................................................................................................13

MICROFLUIDIC-C-RISER (Optional) ............................................................................................15

Connecting the Chamber to the adapter ...................................................................................16

Connection of the Chamber with XY stage .................................................................................17

Cleaning ..........................................................................................................................18

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Summary of Contents for H301-OLYMPUS-IX3-SVR

Page 1: ...VR User Manual REV 03 www oko lab com Page 1 H301 OLYMPUS IX3 SVR Compatible with the following XY stage Compatible with the following Okolab Controllers Olympus IX3 SVR H301 T BL PLUS UNO T UNO T H PREMIXED UNO T H CO2 ...

Page 2: ... Temperature Sensor 7 5 Insertion of Sample Holder into Chamber 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks 9 7 Working with 1x3 and 1x2 chamber slides magnetic locks 11 8 Working with MW Plates Magnetic locks and Chamber riser 12 9 Connection of the Gas Supply 12 10 Working with Perfusion 13 10 1 MICROFLUIDIC C RISER Optional 15 11 Connecting the Chamber to the adapte...

Page 3: ...ith the chamber riser Chamber top view Sliding Lid Chamber Riser Sample Holder Order Separately Chamber Base Adapter Figure 1 H301 OLYMPUS IX3 SVR Components and Dimensions 2 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1x35 M 1 35mm ...

Page 4: ... 1in x2in chambered cover glass and 1 60mm Petri dish LABTEK II 60 M 1 Lab Tek II 1in x2in chambered cover glass and 1 60mm Petri dish GS60 M 1 1x3in chamber slide and 1 60mm Petri dish 6035 M 1 60mm Petri dish and 1 35mm Petri dish 6MW 6 well plates 12MW 12 well plates 24MW 24 well plates 24MW NUNC 24 well NUNC GREINER plates 48MW 48 well plates 96MW 96 well plates Includes MW OIL MW OIL Open fra...

Page 5: ... not included with H301 OLYMPUS IX3 SVR 3 1 Sliding lid The Sliding Lid is a heated glass lid allowing for easy sample loading and pipetting The Sliding Lid is screwed onto the chamber Figure 3 shows chamber dimensions with the Sliding Lid with and without chamber riser Figure 4 illustrates how to remove Sliding Lid when a different Lid is necessary NOTE Sliding Lid MUST BE REMOVED when using any ...

Page 6: ...ser Distance between focal plane and upper side of the glass lid without the chamber riser Distance between focal plane and upper side of the glass lid with the chamber riser Chamber top view H301 KOEHLER LID Figure 5 H301 OLYMPUS IX3 SVR H301 KOEHLER LID 3 3 Injection Lid The Injection Lid is a heated glass lid with two small openings sealed with flexible plastic allowing injection or perma nent ...

Page 7: ...s lifted NOTE Compatible with sample holder 1x35 ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 7 shows chamber dimensions with the Laser Interlock Lid with and without chamber riser Distance between focal plane and upper side of the glass lid without the chamber riser Distance between focal plane and upper side of the glass lid with the chamber ris...

Page 8: ...r 5 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 9 Insert the adapter by superimposing the red points Figure 9 Introduction of the Specimen Holde...

Page 9: ... Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included Figure 11 shows how to position the ring Ring selection guide diagrams rings for 35 and 60 mm dish Figure 12 and Figure 13 respectively 1 3 2 Figure 11 1 Insertion of the ring 2 insertion of the 35 mm ...

Page 10: ...co 35x10 Grainer Petri35x10 Ibidi μ Dish 35 mm low MatTek P35G X14 X Corning 35x10 mm Figure 12 Ring selection scheme for 35 mm dish Corning 60x15 mm BD Falcon 60x10 Ibidi μ Dish 50 mm low Grainer Petri 60x15 MatTek P50G X30 X Willco 60x15 Figure 13 Ring selection scheme for 60 mm dish ...

Page 11: ...s Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 1 2 Figure 14 Magnetic lock for 1 x 3 chamber slide 1 2 3 Figure 15 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 ...

Page 12: ...QUIRED with 6 12 24 48 MW plates regardless of the Lid em ployed Figure 17 shows how to install and fasten the chamber riser Screws location is indicated by letter A A Chamber Riser A Chamber Base Figure 17 Riser Assembly 9 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H301 OLYMPUS IX3 SVR Silicon tubing connects to a gas input brass ...

Page 13: ...insertion of perfusion tubing up to 2 5 mm in outer diameter To lock the Chamber Riser into the base use a 1 5mm Allen key to secure the 4 GUTB2 2 5 6 screws as shown in Figure 19 Figure 19 How to lock the Chamber Riser Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws with a 1 5 mm Allen key as necessary before introducing perfusion tubing Figure 20 shows ...

Page 14: ...H301 OLYMPUS IX3 SVR User Manual REV 03 www oko lab com Page 14 1 Frontal view 2 3D view Perfusion dedicated opening Figure 20 Perfusion Figure 21 How to access the perfusion holes ...

Page 15: ... with 19x3 mm windows on the two short sides to allow insertion of microfluidic tubings Windows are magnetically closed Compatible with most of H201 and H301 Okolab chambers Thickness of this chamber riser is 8mm 2mm more than default chamber riser Requires Koehler lid and single ac commodation specimen holders Figure 22 MICROFLUIDIC C RISER ...

Page 16: ...captive screws in the proper housings 2 Place the chamber on the adapter as shown in Figure 23 Image 3 3 Use a 1 5mm metric Allen Wrench to tighten two of the four captive screws in place A in Image 4 while keeping the sliding lid closed See Figure 23 Image 4 4 Tighten the remaining two captive screws A in Image 5 while keeping the chamber sliding lid open 5 Image 6 of Figure 23 shows the chamber ...

Page 17: ...Figure 24 1 Place the adapter on the stage See Image 1 of Figure 24 2 Follow the instructions shown in Image 2 of Figure 24 for placing socket head cap screws use the provided Allen wrench for tightening the socket head cap screws and fastening the adapter on the stage Screws housings are in dicated with letter A in Image 2 of Figure 24 1 2 Screw M3x6 A A Figure 24 How to fix the H301 OLYMPUS IX3 ...

Page 18: ...he chamber cools down To clean the body and the glass lid of the chamber wipe with a soft micro fiber cloth For stubborn smudges you can damp the soft micro fiber cloth with ethyl alcohol product code UN1170 Do not put any liquid di rectly on the chamber While cleaning the glass lid do not apply strong force to the surface of the glass lid because it can be damaged H301 OLYMPUS IX3 SVR_User Manual...

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