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H301-MINI

User Manual

Rev 02

www.oko-lab.com

Pa

ge

1

H301-MINI

Compatible with the following XY stages

Compatible with the following Okolab Controllers

•

Physik Instrumente: P-737.1SL/2SL/5SL

•

Ludl 99A600

•

It fits in any MW plate holder

•

H301-T-BL-PLUS

•

UNO-T

•

UNO-T-H-PREMIXED

•

UNO-T-H-CO2

•

H401-T-DUAL

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Summary of Contents for H301-MINI

Page 1: ...age 1 H301 MINI Compatible with the following XY stages Compatible with the following Okolab Controllers Physik Instrumente P 737 1SL 2SL 5SL Ludl 99A600 It fits in any MW plate holder H301 T BL PLUS...

Page 2: ...rtion of the Sample Feedback Temperature Sensor 4 4 Insertion of Sample Holder into Chamber 5 5 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks 5 6 Working with 1x3 and 1x2 chambe...

Page 3: ...nts Chamber main body with embedded temperature sensor Heated glass lid with Indium Tin Oxide ITO conductive coating and embedded temperature sensor Distance between focal plane and upper side of the...

Page 4: ...M single 1 60mm Petri dish 1x35 M single For 1 35mm Petri dish holder 1x60 M single For 1 60mm Petri dish holder 1xGS M single For 1 1 x3 chamber slide holders 1xLABTEK M single For 1 1 x2 Labtek cha...

Page 5: ...or 60 mm Petri Dish Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 5 illustrates the available magnetic locks for 35 and 60 mm...

Page 6: ...ck NOTE Spacing rings are included with sample holder Grainer Petri35x10 Ibidi Dish35mm low Corning 35x10mm MatTek P35G X 14 X Willco 35x10 BD Falcon 35x10 Figure 7 Ring selection scheme for 35 mm dis...

Page 7: ...etic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 1 2 Figure 9 Magnetic lock for 1 x 3 chamber slide 1 3 2 F...

Page 8: ...ilicon tub ing onto brass opening Gas Input Figure 11 Connection with gas supply 8 Working with Perfusion The Chamber H301 MINI features 12 perfusion holes on the sides for the insertion of perfusion...

Page 9: ...ber on the stage follow the steps shown in Figure 13 1 Place the chamber on the stage See Image 1 of Figure 13 2 Incline the chamber and slide it in direction of the springs until it snaps into the ho...

Page 10: ...e chamber cools down To clean the body and the glass lid of the chamber wipe with a soft micro fiber cloth For stubborn smudges you can damp the soft micro fiber cloth with ethyl alcohol product code...

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