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H301-ALPHA3-SMALL 

User Manual 

   

REV 02

 

 

 www.oko-lab.com 

  

 

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6

 

5.

 

Installing the H301-ALPHA3-SMALL around the sample 
holder 

To install the H301-ALPHA3-SMALL on the stage, please follow the steps below: 

3.

 

Insert the chamber base by sliding into place as shown in image 1 and 2 in Figure 5. 

Note:

 Please pay attention to the sample holder glass.  

4.

 

Tighten the captive screw M2 to fix the chamber base around the sample holder by using the provided hexagonal 

key (see image 2 in Figure 5). 

5.

 

Place the magnetic lid on the chamber base (see image 3 in Figure 5). 

6.

 

Insert the sample arm cover (see image 4 in Figure 5), and close it (see image 5 in Figure 5). It allows to reduce 

the brightness into the sample.  

7.

 

Place the magnetic objective heater in place as shown in image 6 in Figure 5. 

Summary of Contents for H301-ALPHA3-SMALL

Page 1: ...ser Manual REV 02 www oko lab com Page 1 H301 ALPHA3 SMALL Compatible with the following microscope Compatible with the following Okolab Controllers PhaseView Alpha3 Light Sheet microscope Bold Line U...

Page 2: ...www oko lab com Page 2 Index 1 Components and dimensions 3 2 Insertion of the Sample Feedback Temperature Sensor 4 3 Connection of the Gas Supply 4 4 Working with perfusion 5 5 Installing the H301 ALP...

Page 3: ...ons H301 ALPHA3 SMALL includes the following components Objective Collar compatible with water immersion objective 21 mm diameter Sample Arm Cover Chamber Lid Chamber Base Chamber Base Objective Colla...

Page 4: ...ape on the sample holder inside the H301 ALPHA3 SMALL For more information about the self calibration please refer to the User Manual of the Okolab Controller in use Figure 2 Insertion of the temperat...

Page 5: ...with perfusion 1 Use the supplied Stainless Steel Perfusion Tubes Attach a Silicon tubes O D 1 6mm I D 0 8mm not supplied to the Stainless Steel Perfusion Tubes see image 1 in Figure 4 2 Insert the S...

Page 6: ...Note Please pay attention to the sample holder glass 4 Tighten the captive screw M2 to fix the chamber base around the sample holder by using the provided hexagonal key see image 2 in Figure 5 5 Place...

Page 7: ...H301 ALPHA3 SMALL User Manual REV 02 www oko lab com Page 7 5 4 2 1 3 7 6 Figure 5 Connection of the Chamber with Stage H301 ALPHA3 SMALL_User Manual Rev 02_Published in September 2020...

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