MNL-1012 Rev A
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Calibration
An EEPROM flash memory chip in each Flame contains wavelength calibration coefficients, linearity coefficients, and a serial number
unique to each spectrometer. The spectroscopy application reads these values directly from the spectrometer, enabling the ability to
“hot
-
swap” spectrometers between computers without entering the spectrometer coefficients manually on each computer.
Wavelength Calibration
Each spectrometer is calibrated before it leaves Ocean Insight, however the wavelength for all spectrometers will drift slightly as a
function of time and environmental conditions.
For instructions on how to calibrate the Flame, visit
Irradiance Calibrations
Irradiance calibrations and relative irradiance calibrations are about quantifying the spectra, by translating the signal (incident
number of photons) to a calibration. This can be either absolute (an atomic emission light source of known output power) or relative
(corrected for instrument response function but not absolute units). It can be considered a measurement technique and is used
widely in remote sensing, light metrology and anywhere where you wish to characterize the incident light source. Irradiance
calibrations are not required for many techniques because these measure the relative signal changes with respect to the sample and
not the light source.
You can find out more about irradiance calibration techniques at
OceanView has wizards that will step you through absolute irradiance and relative calibrations and more information on these is
located in the OceanView.
Summary of Contents for FLAME
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