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OM0135 

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Air-Jacketed Automatic CO

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 Incubator 

 

Models 

NU-5510 

NU-5510E 

 
 

Operation and Maintenance Manual 

 
 
 

 

 
 
 
 
 

October, 2010 

Revision 12 

(Series 6 & Higher) 

 
 

 

 

 

 

 

 

 
 
 
 

       Manufactured By: 

NuAire, Inc. 

2100 Fernbrook Lane 

Plymouth, MN  55447 

Toll-Free: 1-800-328-3352 

In Minnesota:  (763)-553-1270 

Fax: (763)-553-0459

Summary of Contents for NU-5510

Page 1: ...cubator Models NU 5510 NU 5510E Operation and Maintenance Manual October 2010 Revision 12 Series 6 Higher Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll Free 1 800 328 3352 In M...

Page 2: ...n 9 1 Chamber Temperature Calibration 9 2 Door Temperature Calibration 9 3 CO2 Calibration Section No 10 Maintaining Your DHD Autoflow 10 1 Heated Decon Cycles 10 2 Chemical Decontamination Section No...

Page 3: ...ity insulation that has a high R rating covers the complete outer surfaces of the Incubator inner chamber 1 2 Incubator Blower Fan HEPA Filter A continuous operating fan motor drives a blower wheel wi...

Page 4: ...crevice free construction which provides an easily cleanable inert surface that does not in itself promote biological growth In addition all shelves shelf supports guide rails and the air plenum are...

Page 5: ...chamber plenum shelves and rails for sterilization Most electronics motors pumps and valves are fully accessible from the front of the unit A CO2 sample port is provided on the front panel to check t...

Page 6: ...Liters Recommended Fill 5 5 Liters 3 2 Performance Parameters Temperature Range 18 C to 55 C 5 C above ambient to 30 C ambient max Temperature Uniformity 0 3 C 37 C Temperature Accuracy 0 1 C Minimum...

Page 7: ...nual Operating Instructions 3 4 Accessories Ordered Separately Model NU 1550 Automatic Tank Switch External 115 VAC Model NU 1550E Automatic Tank Switch External 230 VAC Model NU 1552 CO2 Tank Alarm 1...

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Page 9: ...1 0 milliamperes 4 2 2 Dielectric Voltage Withstand 1770 volts VDC ONLY is applied between dead metal parts and the hot neutral power source lead with no electrical breakdown using an Associated Resea...

Page 10: ...ct NuAire for a Return Authorization Number This number must accompany all returns Any product shipped to NuAire without this number will be returned refused shipment or collect freight 6 0 Shipments...

Page 11: ...an internal temperature variation of less than 0 2 degrees C a minimum of 2 inches 50mm must be allowed between the rear and sides of the Incubator and any walls partitions or obstructions to facilita...

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Page 13: ...sidewall plenums If the shelf does not bind or disengage from the horizontal bracket it is installed correctly Additional shelves and shelf guides are available The water pan shall be placed directly...

Page 14: ...ot be connected to this equipment CO2 gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve Check the gas cylinder for the proper identification labels Do not...

Page 15: ...d on the Incubator back panel labeled CO2 tank 1 The filter should be inserted downstream of the low stage regulator before the inlet fitting on the Incubator as shown in the figure Secure each connec...

Page 16: ...lled water no purer than 1 Mega Ohm It is recommended to fill the pan to about 1 2 inch below the top rim See Sections 3 1 and 8 2 for more specifications and operational details 7 9 Stacking Units Re...

Page 17: ...process of water evaporation vapor water pressure from a stainless steel water pan placed on the bottom of the chamber Materials of different thermal resistance i e glass plastic do not offer suffici...

Page 18: ...8 8 1 2 for instructions To alleviate the condensation increase the airflow To increase RH in the chamber reduce the airflow If airflow is decreased some condensation may be unavoidable The port plug...

Page 19: ...and SEt UP Changes in the set point values are performed in this mode by pressing the SEL key to choose the system indicated by lighting the green LED next to the display and using the up down arrows...

Page 20: ...ed by pressing and holding the SETUP key for three seconds to access the setup mode 8 3 6 Provision for add on expansion capability Chart recorder output option Automatic tank switch option RS 232 out...

Page 21: ...w measurement of CO2 percentage manually with a fyrite CO2 indicator or other suitable instrument 8 4 9 Parameter Indicators LED The parameter indicators located next to the TEMPERATURE CO2 WINDOWS in...

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Page 23: ...sure to insure proper flow rates and consistent CO2 percentage readings 8 5 4 Air Inlet The Incubator is provided with clear vinyl tubing and a 0 3 micron HEPA filter This is a free air supply DO NOT...

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Page 25: ...normal If the blinking green LED does come back on use the troubleshooting guide to correct the abnormality see Section 11 0 8 7 Setup Mode Operator Interactions Pressing the RUN SETUP key for three...

Page 26: ...ch have several function parameters as described below The rst menu performs a master reset function which clears the microprocessor s memory and resets all parameters to their default condition To en...

Page 27: ...the chamber control temperature sensor on the CO2 display This function also allows the chamber heater to be turned on at different percentages 0 25 50 75 100 flashing the temperature display Use the...

Page 28: ...ting with the PEr indicator 10 Air Inject Valve This function shows the current state of the air inject valve The CO2 percent display will show on or off corresponding to the relay condition 11 Air Pu...

Page 29: ...30 16 Temperature Recovery Delay Time seconds 30 17 Temp Sensor Differential C 4 0 18 Temp Max Above Set point C 1 0 19 CO2 Max Above Set point 1 0 Note Default values are in bold Once you have made y...

Page 30: ...e section 9 4 2 5 Alarm Audible Enable This function will enable or disable the audible alarm ring back function The value display will show ON or OFF corresponding to the current condition If the fun...

Page 31: ...menu Enter your password When front panel message displays Ent Pas Re enter your password when front panel message displays Pas rEq Press mode key to SETUP then back to run 8 Auto Zero Enable This fun...

Page 32: ...t get to within 0 2 of set point within this time period an alarm condition is declared 16 Temperature Recovery Delay Time This value determines the time in seconds to turn off the main heater in half...

Page 33: ...eviation measured in CO2 percent that the chamber is permitted above or below once the Incubator reaches the specified set point before an alarm condition is declared 8 8 1 3 Reset Master The master r...

Page 34: ...r resting on a shelf in the middle of the DHD Autoflow chamber Do not place the glass beaker on the bottom of the chamber because it will result in a slightly higher temperature reading due to the hea...

Page 35: ...ould be made at a time Press SEL to set current value and exit calibration 9 2 1 Door and Perimeter heater duty cycle automatic control The door and perimeter duty cycles are automatically reduced whe...

Page 36: ...RE THAN ONCE PER WEEK Sensor calibration should be performed if an independent measurement doesn t match the display within 0 3 percent within one week after a sensor calibration Before doing the foll...

Page 37: ...splay Open the inner and outer doors then push the until dor appears in the temperature display When dor starts flashing after 90 seconds close the doors Display shows old value if other than zero the...

Page 38: ...running the remaining steps listed below Then run the zero span routine again checking to make sure that the display reaches zero The CO2 display will show the following in order Span Calibration DLY...

Page 39: ...the CO2 sensor HEPA filtered room air is pumped through the sensor detector cell for 2 minutes The CO2 reading is checked at this time If it 0 5 or less different than the current zero the sensor wil...

Page 40: ...should be performed periodically and during CO2 sensor calibration to assure filter integrity A visual check would include removing the plenum top and observing the HEPA filter interior and exterior f...

Page 41: ...he CO2 IR sensor since it is remote from the chamber See the instructions above for choosing this cycle in the options menu OUTER DOOR MUST REMAIN CLOSED DURING THE DOCONTAMINATION CYCLE OR IF CHAMBER...

Page 42: ...n and clean the chamber When trying to advance the Incubator will beep rapidly and dor will show in the Temperature display HEAT UP 3 hours Press the 95 145 button again to start the heat up portion o...

Page 43: ...phase of either the 95 or 145 decontamination cycles press the 95 145 button to step through the different phases of the cycle that are described above The cool down phase cannot be bypassed until te...

Page 44: ...E OUTER DOOR DURING THIS CYCLE CAN RESULT IN BURNS PREPERATION Remove culture cells samples dishes instruments or other user introduced equipment from the chamber Wipe out all spills and materials fro...

Page 45: ...r and the outer doors Note CO2 system will be idle during the cycle Protection of the sensor during the heated cycle is automatic The Decon Cycle will not advance to the heat up phase unless the door...

Page 46: ...ver the 95 145 button is lit continuously COOL DOWN 3 hours When the humidity reduction period is complete the heaters are shut off to cool the chamber to the user chosen temperature set point Tempera...

Page 47: ...may use the traditional formaldehyde Vapor Phased Hydrogen Peroxide or Chlorine Dioxide All three of the chemicals are compatible to all parts within NuAire Incubators NOTE As stated previously the ch...

Page 48: ...ture sensor calibration 2 Replace fuse 3 Faulty TRIAC replace control board 4 Faulty chamber heater contact NuAire Technical Service 5 Door Perimeter heater needs to be increased with a high temperatu...

Page 49: ...d does not affect the operation of the CO2 system 1 Zero span calibrate the CO2 sensor 2 Check ambient CO2 level Ventilate area if level exceeds normal limits 3 Check air inject system function 4 Chec...

Page 50: ...LAY Interruption of power 1 Press any key to stop blinking displays Glass door gasket or front wall of chamber wet 1 Increase door and front perimeter heater duty cycles See section 9 for detailed ins...

Page 51: ...elephone jack and rated for 30V at 1 Amp The contacts provided are normally open NO normally closed NC and common COM as shown below The alarm contacts do not distinguish between a CO2 temperature or...

Page 52: ...in the operating environment Pollution degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation 13 6 Chemi...

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