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OM0196 

 
 
 
 

CELLGARD ES Energy Saver Class II,  

Type A2 Laminar Flow 

Biological Safety Cabinet 

 

Models 

NU-475-300/400/500/600 

Bench/Console 

 

Operation & Maintenance Manual 

 
 
 
 
 
 

January, 2011 

Revision 1 

(Series 1 & Higher) 

 
 

 
 
 

 

                  
 
 

 
 
 
 
 
 

Manufactured by: 

NuAire, Inc. 

2100 Fernbrook Lane 

Plymouth, Minnesota USA 55447 

Toll Free: 1-800-328-3352 

In MN: 763-553-1270 

Fax: 763-553-0459 

NU-475-400/500/600 Only 

Summary of Contents for NU-475-300

Page 1: ...abinet Models NU 475 300 400 500 600 Bench Console Operation Maintenance Manual January 2011 Revision 1 Series 1 Higher Manufactured by NuAire Inc 2100 Fernbrook Lane Plymouth Minnesota USA 55447 Toll...

Page 2: ...e contaminants as prescribed in NSF ANSI 49 Please read this manual carefully to familiarize you with proper installation maintenance and operation of the cabinet Other reference and guideline materia...

Page 3: ...are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended to this...

Page 4: ...A Filter Leak Test 7 7 Airflow Smoke Pattern Test 7 8 Site Installation Assessment Tests 7 9 Cleanliness Classification Test for Pharmacy Application Section No 8 Error Indicators Troubleshooting Sect...

Page 5: ...en used with proper technique is an effective laboratory aid in obtaining the optimum control over product quality while reducing the potential for exposure of both product and personnel to airborne b...

Page 6: ...o which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these operating instructions close to the uni...

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Page 8: ...OM0196 8 2 0 Models Features The model NU 475 Class II Type A2 Laminar Flow Biological Safety Cabinet is manufactured in four sizes 3 ft 4 ft 5 ft and 6 ft...

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Page 13: ...any item for any reason contact NuAire for a Return Authorization Number This number must accompany all returns Any product shipped to NuAire without this number will be returned refused shipment or c...

Page 14: ...HE CEILING FOR PROPER VENTILATION OF THE EXHAUST EFFLUX If this cabinet is used in a pharmacy application Per OSHA NIOSH and ASHP it is strongly recommended that the cabinet be exhausted to the outsid...

Page 15: ...e is desired remove the handle from the valve stem to gain clearance for valve body rotation Add Loctite 242 furnished to the threads and rotate valve body until secure with the valve stem for handle...

Page 16: ...cabinet if necessary Service ball valves should never be used for flammable gasses or oxygen service A special needle valve for oxygen service or certified valve is required and available upon request...

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Page 18: ...a soft cloth Turn the cabinet on and let it operate for 60 minutes before using it as a LFBSC 5 3 Certification Testing Methods and Equipment After installation and prior to use NuAire recommends tha...

Page 19: ...t Fully Assembled Height Minimum for Transport Width Depth with Control Center And Armrest Removed 61 7 8 1572 59 1499 41 5 8 1057 33 838 61 7 8 1572 59 1499 53 5 8 1362 33 838 61 7 8 1572 59 1499 65...

Page 20: ...optional With the U V light option the switch provides for on center off on operation so that both the fluorescent light and the U V light cannot be energized at the same time The UV light is also in...

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Page 22: ...PLOYING THE PROPER INSTRUMENTS IN ORDER TO INSURE AIRFLOWS PER NSF ANSI 49 6 1 9 Minihelic Gauge The unit is equipped with a minihelic gauge The minihelic gauge displays the static pressure within the...

Page 23: ...prevent overloading but the work should also be planned to minimize the number of times an operator s hands and arms must enter and leave the air curtain at the open face The ideal situation is to hav...

Page 24: ...to the downflow to minimize capture of chance particulates This precaution is merely an extension of good aseptic technique as practiced on open bench tops The good laboratory practices designed to mi...

Page 25: ...n glassware should be avoided and transfer of viable materials should be performed as deeply into the cabinet away from open face as possible 6 3 4 Air Purge Additional purging of the workspace withou...

Page 26: ...hen working in a cabinet Evaluation criteria should be at a minimum a Proper user posture b Effective workzone layout for work practice c Vision or sightlines For each of the above evaluation criterio...

Page 27: ...et remains in operational mode with internal blower on b Open the hinged or sliding view screen and secure in the full open position With the view screen in the full open position personnel protection...

Page 28: ...he supply HEPA filter d Wipe down all exposed surfaces of the work area with 70 isopropyl alcohol e Prepare for aseptic operation 1 Available from Lab Safety Supply Janesville WI 53547 1368 or other l...

Page 29: ...evaporates immediately after reaching its boiling point the minimum reaction time is 6 hours Therefore the formaldehyde should be neutralized after the specified reaction time by vaporizing ammonia F...

Page 30: ...The motor is lubricated for life and is thermally protected with automatic reset 7 2 Fluorescent Lamp Bulb Replacement The two T8 fluorescent bulbs are cool white rapid start and placed external to t...

Page 31: ...ws are removed the panel is held into position by smooth weldstuds located on the top corner of the front filter panel Use the window stop brackets as handles to remove the panel NOTE Screws are used...

Page 32: ...kets of the filter and carefully insert into the exhaust choke tray Position the filter frame within the outside walls of the exhaust opening on the top of the hood Tighten the spring loaded bolts 4 p...

Page 33: ...le by a set screw and tension screw method see Drawing BCD 11818 When adjusting the sliding window be sure to verify proper microswitch operation If the sliding window is too loose the sliding window...

Page 34: ...calibration mode The second version see photo below provides more feedback to the Service Technician in that there is a calibration mode push button next to the potentiometer that when pushed activate...

Page 35: ...the reduced speed took away the choke plate restores Note The choke plate adjustment requires a standard blade screwdriver To adjust loosen the liquid tight fitting around the choke adjustment shaft...

Page 36: ...ize Supply Area ft2 m2 Exhaust Area ft2 m2 300 6 33 588 1 61 150 400 8 53 792 2 38 221 500 10 74 998 3 15 293 600 12 95 1 203 3 92 364 Measured 4 inches above the bottom edge of the window Laskin Nozz...

Page 37: ...larm manufacturer procedures Step 5 Return the speed control to its certified operating voltage as measured by the voltmeter 7 8 3 Exhaust System Performance This test is broken up into two groups bas...

Page 38: ...n hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Take 5 test poi...

Page 39: ...ceptance Criteria 1 Average downflow velocity 55 to 65 fpm 28 to 33 m s 2 Individual readings must be within 20 or 16fpm 0 08m s which ever is greater factory test or 25 or 16fpm 0 08m s which ever is...

Page 40: ...15 421 528 635 741 848 955 1061 1168 1274 1381 4 8 158 12 316 16 474 20 632 24 790 28 948 33 106 37 264 41 422 45 580 49 738 53 896 58 054 62 212 66 375 600 102 207 313 418 524 630 735 841 946 1052 11...

Page 41: ...correct the situation Error Indicator Troubleshooting Guide Error Indicator Indicator Correction Window alarm Sliding window is above it s standard working height or microswitch is not operating prope...

Page 42: ...t of circuit breaker Replace circuit breaker Isolate output of circuit breaker by disconnecting control center connectors light circuit motor voltage regulator etc to isolate the short Minihelic gauge...

Page 43: ...nnel leave the cabinet face area 10 1 2 Operation The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch location on the front panel The slidin...

Page 44: ...cus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Proteus vulgaris 7...

Page 45: ...es and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degree 2 0 Pollution degree describes the amount of con...

Page 46: ...ss Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative Panel Painted...

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