SEV-00184-01
nCounter Pro Analysis System User Manual
FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
41
Cartridge Definition Files
A Cartridge Definition File (CDF) defines sample-specific data to associate with the data output and
defines the parameters the Digital Analyzer will use during image collection and processing. Data
contained in the CDF include:
Data Field
Description
LaneID
Defines which lanes in the cartridge will be scanned. If all twelve lanes will be
scanned, then this should not be changed. If only a subset of the lanes will be
scanned, then the information for the empty lanes can be deleted.
SampleID
Required. Sample identifiers can be specific names or assigned default names
01–12 corresponding to one or more of the 12 lanes on the sample cartridge.
The sample ID is displayed as part of the Reporter Code Count (RCC) file name
and as a column heading in the data output file.
Owner
Optional. Example uses include the name of individual running the experiment
or the study’s Principal Investigator. Details are output within the RCC file as
general information.
Comments
Optional. Example uses include details regarding the project or a specific
sample. Details are output within the RCC file as general information.
Date
Optional. This field can contain any date in the YYYYMMDD format. The date
of a scan is automatically added to the beginning of the RCC file name, so it is
not required here.
FOVCount
Required. This field specifies the number of images (fields of view) to analyze
per assay, which corresponds to the amount of data to collect. Options are
555, 280, 100, or 25.
GeneRLF
Required. The RLF contains the code key information used during image
processing to assign target identities to the barcodes. If this file is not
designated, the system will not allow data collection. Each RLF is unique to a
CodeSet, so the correct RLF is critical. The “.rlf” file type extension should NOT
be used here.
IMPORTANT:
The version of the RLF must match the version of the CodeSet used in the
hybridization.
Users can create a new CDF on a personal computer or directly on the Digital Analyzer using the
touchscreen. NanoString recommends users create CDFs on their computer if detailed information
for each sample is required. In this case, a USB flash drive can be used to transfer the CDF to the
imaging instrument. However, if the Enterprise Package is enabled, some configurations may require
users to transfer the CDF via FTPS (see the
section).
Summary of Contents for nCounter Pro
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