NanoEnTek ADAMII-CD34 User Manual Download

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User manual

Bench-top fluorescence cell counter

-CD34

Summary of Contents for ADAMII-CD34

Page 1: ...User manual Bench top fluorescence cell counter CD34...

Page 2: ...out notice as part of ongoing product development ADAMII User Manual Website www nanoentek com E mail ivdst nanoentek com Manufactured by NanoEntek Inc 851 14 Seohae ro Paltan myeon Hwaseong si Gyeong...

Page 3: ...e menu 10 11 Calibration 12 19 Measurement 20 33 Data 34 43 Setting 44 48 Cleaning Maintenance 49 Troubleshooting 50 51 Error code 52 53 Warranty 54 Safety precautions 55 Consignes de securite 56 Safe...

Page 4: ...CD34 is a CE approved instrument that can be used for mobilized peripheral blood MPB and hematopoietic progenitor cells apheresis HPC A samples ADAMII CD34 reports absolute counts of total and viable...

Page 5: ...l sale representative or send an email to ivdst nanoentek com Product components ADAMII CD34 1 EA User manual 1 EA Focus guide 1 EA 4 pcs 1 SET Power cord with 4 adaptor cords for U S Canada Taiwan Ja...

Page 6: ...Slide door State LED Blue LED lights up when the instrument is connected to the laptop and the ADAMII CD34 software is running State LED Where one can access the Slide holder Where ADAMII CD34 Assay...

Page 7: ...description Rear view To connect USB cable to communicate with the laptop To turn power On and Off To connect the power adapter USB 3 0 port Power supply port Power switch Power supply port Power swi...

Page 8: ...able and level surface 1 Plug the power cord into an electrical outlet and plug the DC output jack to the power supply port 2 Connect USB 3 0 cable to the USB ports in the ADAMII CD34 and the laptop 2...

Page 9: ...d ON before starting ADAMII CD34 software in the laptop If the instrument is not on when the software starts the software will give a warning after searching the instrument for more than 10 seconds No...

Page 10: ...g in Default ID and password are both master Click User management when you need to manage accounts e g to add a new user or to change password To create a new ID fill out the blanks user name user ID...

Page 11: ...trail to search and trace detailed information of each user s activities 1 Enter the User ID and set the date that you want to search 2 Click Search button 3 The record of the selected user is displa...

Page 12: ...he ADAM II CD34 first time the instrument needs to be calibrated Subsequently it is recommended that the calibration is done regularly or at least once a week A pop up window for calibration appears a...

Page 13: ...ed in ADAMII CD34 kit Before use the ADAMII Assay Slide should be removed from refrigerator and kept at room temperature for at least 30 minutes The reagents should be kept at 4 C 3 Once again vortex...

Page 14: ...ng samples to the slide please release sample gently and slowly to avoid bubbles because bubbles interfere image analysis 5 Leave for 20 seconds Calibration Beads needs time to settle 6 To insert the...

Page 15: ...number has to be entered after inserting slide Calibration Calibration procedure 7 Enter lot number of Calibration Beads 8 Select Bright channel and adjust the focus using the focus buttons To view b...

Page 16: ...ds or blurry boundaries around beads FL Good Focus Compared to dark background white inside of beads and crisp boundaries around beads FL Bad Focus gray inside of beads and fuzzy boundaries Good Focus...

Page 17: ...r adjusting focus 10 Select FL3 channel and adjust the focus using the focus button To view cells 2x bigger press the ZOOM IN button 12 Press the Run button Note After saving the focuses of Bright and...

Page 18: ...ed 14 You can see the previous record of calibration by clicking Record button Note If any of the results are FAIL please contact the manufacturer or distributor Note If any of the results are Low ple...

Page 19: ...ADAMII NanoEntek Inc 19 Calibration Calibration procedure 15 The calibration results are displayed To save the results click Save button 16 Press Close button to finish calibration...

Page 20: ...asure a sample click Measure menu Load 25 uL stained sample into an ADAMIITM Assay Slide Load 25 L sample Note Please refer to page 14 to check cautions for loading correct volume Note Before loading...

Page 21: ...say slide Measurement 1 Open the slide door 2 Press the black bar to open the slide holder 3 Insert the Assay Slide into the slide holder Note Assay Slide should be inserted all the way in Please see...

Page 22: ...are presented below 4 Close the slide holder by pressing down the top portion of the holder tightly until it clicks Warning If the slide holder is not properly closed ADAMII CD34 will give a warning...

Page 23: ...r and the number of frames 7 Press the Insert sample button on the ADAMII CD34 software The slide holder containing the Assay Slide will move into the measuring position Warning Do not look inside the...

Page 24: ...ions of each part in the Measure menu are as follows 1 Counting mode 2 Display area 3 Detection channel 4 Insert sample 5 Counting results area 6 Test information Measurement Measure menu Initial mode...

Page 25: ...frames of images per sample Viability Check the circle to include viability measurement Live feed of camera will be shown in this window The window will be activated after inserting slide Refer to pa...

Page 26: ...g image acquisition and analysis the results will be displayed here Refer to page 33 Detailed information of the sample will be displayed here Measurement Measure menu initial mode Counting result are...

Page 27: ...II NanoEntek Inc 27 Once the Assay Slide is inserted focusing mode will appear 1 Display area 2 Channel 3 Focus option 4 Save focus 5 Run sample 6 Eject Measurement Measure menu Focusing mode 6 2 3 1...

Page 28: ...being displayed CD45 CD34 Viability for live dead marker 1 Focus can be adjusted by clicking up or down arrow in Move Focus Measurement Measure menu Focusing mode Display area Channel Focus option No...

Page 29: ...will be manually adjusted and the focuses for the other 2 fluorescence channels will be automatically set based on the CD45 focus position Auto focus If you press the Auto focus button the focusing w...

Page 30: ...d focus 1 Compared to gray background the inside of cells is not dark and the boundaries are not bright and clear Bad focus 2 The inside of cells is white Bad focus 3 While the inside of cells is dark...

Page 31: ...Focus VS Bad Focus Focusing example fluorescence channel Good Focus Clear cell image bright cell body in contrast to the dark background Bad focus Unclear cell image blurry cell image CD45 PerCP Bad...

Page 32: ...and dilution factor are selected accurately 2 Press the Run sample button 3 Enter the Sample ID Patient ID Antibody Lot and Slide Lot 4 When the counting is done Assay Slide will be ejected automatic...

Page 33: ...lide door press the button on the slide holder to open the holder remove the used slide insert a new slide and repeat the steps Click Eject button when you want to manually eject the Assay Slide There...

Page 34: ...countings of each channel at each frame the lists of data and an option to export data 1 Test information 2 Sample information 3 Captured image 4 Sample volume information 5 Result area 6 Histogram 7...

Page 35: ...he sample type HPC A Mobilized PB or Control Measured frame Number of frames that were measured Deleted frame Number of frames that were deleted Resulted frame Final number of frames that were used fo...

Page 36: ...ells counted in that channel Click the circle again to make the markers disappeared In one particular channel one can make markers from a different channel appear for example one can choose to see an...

Page 37: ...aptured image Double click the captured image then you can check preview images of selected channel on the right side of the screen To check the pre view images of other channels select that channel a...

Page 38: ...ge insert included in ADAMII CD34 kit HPC A Total volume 275 L sample volume 20 L Mobilized PB Total volume 60 L sample volume 20 L Control Total volume 60 L sample volume 20 L Results area displays t...

Page 39: ...ted data reflecting the deleted frame s Histogram of cell size in each channel is accessible by double clicking small inset of histogram In the pop up window one can change the size gating conditions...

Page 40: ...n a specific area of the sample that frame should be deleted 2 Dust When there is dust inside the slide that frame should be deleted 3 Bubble After loading the sample if there is bubble in the slide t...

Page 41: ...ata 4 Cell debris When there is cell debris that frame should be deleted 5 Overlapped circle When there are several circles in one cell that frame should be deleted Data menu Raw data table Br image F...

Page 42: ...u Data list Edit data list User can edit the Data list Rename To rename a file select the data file and click Rename button to change name of the selected data Select all Click Select all button to se...

Page 43: ...n to export the data in 3 types of formats at once csv file Data table containing comprehensive information in a spreadsheet jpg file Raw images of all 4 channels pdf file A report with summaries of m...

Page 44: ...ral user preferences and other Settings It allows users to change settings including cell counting settings and update software and firmware 1 Date Time 2 Counting settings 3 Calibration 4 Sample volu...

Page 45: ...It is not recommended to adjust the sensitivity and circularity by the user Sensitivity Refers to the contrast of the objects from the background Circularity Used to determine which objects to include...

Page 46: ...ck Record button User can see the records of previous calibrations While we don t recommend users to change this user can change the default sample volume and total volume Only apply when necessary Se...

Page 47: ...ds to be updated 2 If an update version of SW or FW has been provided copy the file in a USB drive and plug it into the laptop 3 After the USB drive appears as an external drive click SW update 4 Find...

Page 48: ...tatus of connection between camera in the instrument and the laptop Disk info indicates available space in the hard drive installed in the laptop Please back up or delete your data from the hard drive...

Page 49: ...TANT Never disassemble or service ADAMII by yourself Unauthorized repairs may damage ADAMII or alter its functionality which will void your warranty Contact ivdst nanoentek com or your local distribut...

Page 50: ...and read again Set the focus with 2ch manual focus function Request technical support First check the sample type Second if you used the bottom of ADAMII CD34 Reagent that almost used please use the...

Page 51: ...otinsertandcounttheslide when the slide holder cap opened But if sensor of slide holder cap is faulty and you start counting without closing the cap then the result will not be accurate When you eject...

Page 52: ...n fail E0010 Serial disconnected E0020 Camera disconnected E0030 Slide holder is opened E0031 Slide door is opened Error code Reason Message Position Driver Stage working Camera Slide inserting Slide...

Page 53: ...ADAMII NanoEntek Inc 53 Error code E0040 Data storage is full E0050 Sample density is too high E0060 Update fail Error code Reason Message Position Data save Measuring SW update...

Page 54: ...fittings or spare parts supplied by anyone other than NanoEntek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample For your protection...

Page 55: ...mage of the instrument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the w...

Page 56: ...instrument N inserez jamais aucun objet dans les orifices d aeration de l instrument car cela pourrait entrainer un choc electrique des blessures chez les utilisateurs et des dommages d equipement Bra...

Page 57: ...or Electrical Instrument for Measurement Control and Laboratory Use Part l General Requirements This instrument bearing the T V symbol are certified by T V Product Services to be in conformance with t...

Page 58: ...t Operating power Operating environment Weight Dimensions ADAMII CD34 Light source Bright Green PE Green PerCP and Blue FITC High sensitivity monochrome CCD Automated X Y Z stage CSV PDF JPEG 12VDC 5...

Page 59: ...on ADAM2 ADAMIITM Bench top Fluorescence Cell Counter Main instrument Power cord USB connection cable ADAMII CD34 Reagent 10X RBC Lysis Buffer ADAMII Calibration Beads ADAMII Assay Slide CD34K 025 ADA...

Page 60: ...ation or technical assistance please call or email Email ivdst nanoentek com Website www nanoentek com Manufactured by NanoEntek Inc 851 14 Seohae ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea T...

Page 61: ...ADAMII NanoEntek Inc 61...

Page 62: ...0 7942 Fax 82 2 6220 7999 MT Promedt Consulting GmbH Altenhofstrasse 80 66386 St Ingbert Germany NanoEntek America Inc 220 Bear Hill Road Suite 102 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 79...

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