8
(4). Diopter regulation
Use the diopter ring 1 on the left lens to adjust diopter.
(a) Put the 40X objective in the light path, observe with right eye and
adjust till the specimen image is clear.
(b) Do the same step on left. The diopter range is N=
±
5. See figure
12.
● Phase contrast observation
1.Put specimen
Put the specimen on the stage board or directly on the stage, focus till the image clear.
2.centering
1)Take out one of the eyepiece, insert the eyepiece 2 into the lens as figure 13-a.
2)A dark ring 5 and a bright ring 6 would appear in the field as figure 13-c.
3)Adjust ring 1 if the edge of the dark or bright ring is not clear.
4)Adjust the plate as figure 13-c shows if the two rings not coincide with Allen wrench 3. Adjust screw 4 if it is a
pull-plate one. See figure 13-d.
5)After centering, take out centering eyepiece. And insert observation eyepiece again.
(a)
1
3
(b)
(c)
(d)
Figure 13
2
4
1
Figure 12
The objective should coincide with the plate in the condenser; otherwise the
effect of imaging would be worse.
Caution
Centering again when change magnification to ensure the imaging effect.
Caution
3
4
4
Summary of Contents for MF52-N
Page 2: ...Inverted Fluorescence Microscope MF52 N ...
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