Molecular Devices SpectraMax GEMINI EM, Operator'S Manual

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Chapter 1
1-14 SpectraMax GEMINI EM Operator’s Manual
Speci fi cations
Technical speci fi cations are subject to change without notice.
Fluorescence Photometric Performance
Wavelength range
(Excitation/Emission) 250–850 nm
Wavelength selection Scanning monochromator tunable in 1-nm increments
Excitation wavelength bandwidth 9 nm
Emission wavelength bandwidth 9 nm
Wavelength accuracy < ± 2.0 nm
Calibration Self-calibrating with built-in fluorescence calibrators
Sensitivity
(signal 3X STD DEV of baseline) 8.0 fmol/well FITC (bottom read)
3.0 fmol/well FITC (top read)
Luminescence Photometric Performance
Wavelength range 250–850 nm
Sensitivity
(signal 3X STD DEV of baseline) 10 amol/well Alkaline Phos. (obtained with Emerald II re-
agent from Tropix, a Perkin Elmer company)
General Photometric Performance
Microplate formats 6, 12, 24, 48, 96, 384
Light source Xenon flash lamp (1 joule/flash)
Average lamp lifetime 2 years normal operation (estimate)
Detector Photomultiplier (R-3896)
Read time 96 wells in <15 seconds (measurement type may extend
read time)
Shaker Time 0–999 seconds
Temperature control (chamber) A4°C to 45°C
Ramp up to 37°C <20 minutes
NOTE: