Chapter 13: Plating Processes
5031498 G
181
Setting Source and Destination Options
To select source and destination options:
1. In the Source and Destination Options window, in the Source Microplate Options section, in
the Select Microplate field, select the plate type for the source.
2. In the Number of Samples field, enter the number of samples to aspirate from each plate. If
you specify 48 or fewer samples, only one 48-region QTray is required.
3. In the Replicates field, select the number (1, 2, 4, 8, or 16) of source sample replications
needed.
4. In the Start Well field, select which well is the first from which to aspirate. Alternatively, click
the ... button to select the well in a plate image.
5. In the Destination Petri Dish Options section, in the Select Petri Dish field, select the kind of
Petri dish to use.
6. In the Number Of 48-region QTrays field, enter the number of destination QTrays to use.
7. In the Pipette Options section, in the Number of Replicates Per Tip field, specify how many
times a pipette tip is used in a replication.
8. In the Start Pipette field, select a starting pipette position. Alternatively, click the ... button to
select the well in a plate image.
9. From the Patterns list, select the plating pattern to use for each region.
Spiral does a rotating and enlarging pattern from a central point.
Radial does a multi-directional radiating pattern from a central point.
Left to Right does a pattern where the pins stir the liquid in a left-to-right and
top-to-bottom direction.
10. Click Next to define aspiration volume and depth options.
Setting Volume and Depth Options
To set volume and depth options:
1. In the Volume and Depths Options window, from the Plating Head list, select the head to
use for the plating routine.
2. In the Liquid Volume (ul) field, enter a value from 10 to 130 for the aspiration volume in
microliters (µl).
3. To triturate the liquid, in the Mix Volume (ul) field, enter a value from 10 to 130 for the
dispense and aspirate volume in microliters (µl) and then in the Mix Steps field, enter the
number of times to dispense and aspirate the sample.
4. In the Blowout Volume (ul) field, enter a value from 10 to 130 for the volume in microliters
(µl) to dispense to ensure that all the liquid is dispensed from the pipettors.
5. In the Microplate (mm above bottom) field, enter the distance in millimeters (mm) above the
bottom of the plate where the pipettors start to aspirate the sample.
6. In the Receptacle (mm above agar) field, enter the distance in millimeters (mm) above the
top of the agar in the QTray where the pipettors start to dispense the sample.
7. Under Plating Depth in the Receptacle (mm above agar) field, enter the distance in
millimeters (mm) above the top of the agar in the QTray where the plating head does the
defined plating pattern.
8. Click Next to select a Sanitise profile.
Summary of Contents for QPix 450
Page 1: ...5031498 G August 2022 QPix 450 or QPix 460 Colony Picking System User Guide ...
Page 6: ...QPix 450 or QPix 460 Colony Picking System User Guide 6 5031498 G ...
Page 22: ...QPix 450 or QPix 460 Colony Picking System User Guide 22 5031498 G ...
Page 56: ...QPix 450 or QPix 460 Colony Picking System User Guide 56 5031498 G ...
Page 102: ...QPix 450 or QPix 460 Colony Picking System User Guide 102 5031498 G ...
Page 158: ...QPix 450 or QPix 460 Colony Picking System User Guide 158 5031498 G ...
Page 176: ...QPix 450 or QPix 460 Colony Picking System User Guide 176 5031498 G ...
Page 218: ...QPix 450 or QPix 460 Colony Picking System User Guide 218 5031498 G ...
Page 226: ...QPix 450 or QPix 460 Colony Picking System User Guide 226 5031498 G ...
Page 250: ...QPix 450 or QPix 460 Colony Picking System User Guide 250 5031498 G ...
Page 256: ...QPix 450 or QPix 460 Colony Picking System User Guide 256 5031498 G ...