Molecular Devices QPix 420, User Manual

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Chapter 6: Picking Processes
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8. When the Please Load Source window displays, load the source receptacles in the correct
locations on the instrument deck.
Tip: If needed, the filter must be installed between the lightbed and the source tray
before continuing.
9. Close the instrument door.
10. Click Next to take a white light test image of the source receptacles.
Adjusting the White Light Test Image
In the Test Image window, all detected features are viewed as potential colonies to pick. To
make sure that only colonies are detected, adjust the test image in this window using the
Exposure and Gain settings, the Detection settings, the Display settings, the Debris settings,
and the Depth settings. Each detected feature displays within a yellow ring.
To view the pixel intensity values in the image map to manually set a detection threshold or
detect areas of saturation within the image:
1. Right-click over the image map and select Intensity.
2. Move the cursor over the area of interest in the image map to display the pixel intensity
value.
To adjust the test image:
1. From the Receptacle list, select the number of the receptacle to view and then click the
frame that you want view in the receptacle image below the list. The frame is in red.