Fluidics Option Operations
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Items to Be Provided by the Customer
The customer must provide the following items (in addition to the
Environment Control option items):
•
ANSI standard compound plates.
•
FLIPR Tetra 96 tips (Part 9000-0761, 50 racks/case) or 384 tips
(Part 9000-0763, 50 racks/case). Tips can be ordered from
Molecular Devices.
•
Absorbent pad for liquid waste disposal.
Tips
The ImageXpress Fluidics option is compatible with FLIPR Tetra 96 or 384
tips.
After compounds are dispensed, tips are disposed into the waste
disposal tray located at the back of the environmental enclosure.
Plates
The tip and compound plate region inside the environmental enclosure
can accommodate up to two compound plates. Molecular Devices
recommends using standard 96-well or 384-well plates. U-bottom and V-
bottom plates are beneficial when withdrawing and dispensing very
small volumes of compound.
Setting Up Fluidics Hardware
The FSE (Field Service Engineer) installs the ImageXpress Fluidics option.
After installation, fluidics can be set up for experiments.
To set up the fluidics hardware
1.
Load the tip racks. For proper fit and alignment, insert the right
side first, and then press the left side down into place.
2.
Change the tip adapter as appropriate. The tip adapter should be
screwed on firmly but not over-tightened. If the tip adapter is not
properly attached, it might not match the fluidics robot
calibration.
3.
Load the compound plates. For proper fit and alignment, insert
the right side first, and then press the left side down into place.
4.
Set up environmental control as needed, and allow the
compound-plate temperature to reach equilibrium. See
Up Environmental Control on page 14
.
ImageXpressOptionsUserGuide.book Page 33 Wednesday, September 9, 2015 3:54 PM