Molecular Devices ImageXpress Confocal HT.ai, User Manual

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Chapter 1: ImageXpress Confocal HT.ai System
The ImageXpress® Confocal HT.ai High-Content Imaging System is an integrated cellular
imaging and analysis system designed for rapid, automated screening of fluorescently labeled
biological samples in microplates. With the addition of modular options, the system provides
environmental control for live cell imaging and uses transmitted light capability for label-free
imaging.
The core hardware component of the imaging system is a custom-designed, fully automated,
epi-illumination fluorescence, confocal microscope. The rapid autofocus and precision sample
movement features of the microscope allow large numbers of high-resolution images to be
acquired in the shortest possible time. All key optical and mechanical elements are motorized,
with asynchronous command execution, allowing complete real-time control of the instrument
configuration through the MetaXpress® High-Content Image Acquisition and Analysis Software.
When used in combination with the powerful image analysis capabilities of the MetaXpress
software, the instrument becomes an extremely flexible and programmable device, ideally
suited for user-defined, high-speed automated assays.
Key components of the instrument include the following:
External solid-state laser light source with seven channels connected by a fiber-optic light
guide and selectable dichroic and emission filters inside the instrument
Scientific CMOS camera
Laser autofocus system with precision motorized Z (focus) stage
Image-based autofocus
Precision motorized X-Y (sample) stage
User-changeable, high-quality, Nikon objectives in a four-position linear selector
User-changeable and selectable emission and dichroic filters in two separate controllable
wheels inside the confocal module
Spinning confocal single-disk box with available dual-disk boxes designed for confocal
screening and confocal research
Selectable binning modes to decrease exposure time and increase throughput
Motorized selection of stage position, filters, and objectives with asynchronous operation
High-transmission fluorescence imaging optics with world-class chromatic aberration
correction, resolution, and image flatness
Operation and configuration control by the integrated MetaXpress software
Optional expansion solutions available for environmental control, phase contrast
transmitted light, and water immersion objectives
Optional plate-handling robot with an automated barcode reader
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