Molecular Devices FLIPR Tetra, User Manual

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ScreenWorks Software Overview
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Analysis process is selected, otherwise retaining all the protocol setup
information contained in the protocol file that was used to generate the
data.
Besides showing the settings for the particular processes incorporated
into a file, a one-page protocol summary can be viewed in the
Experiment window with View > Experiment Summary . Revert to
the experiment setup view with View > Experiment Setup .
ScreenWorks Software always opens with a default protocol file,
Untitled 1 , containing four processes. This can be used to start
construction of a new protocol, or closed or ignored if you wish to work
with existing protocol or data files.
Constructing Protocols Using FLIPR Tetra Processes
Protocols are comprised of combinations of the following processes:
• Settings
• Analysis
• Transfer Fluid
• Mix Fluid
• Wash Reservoir
• Wash Tips or Pins
• Blot Pins (Pin Tool only)
• Pause Pipettor
• Finish with Source
• Read
The Settings and Analysis processes are required for every protocol.
They are automatically included as the first two processes in every new
protocol file you create, and cannot be removed.
Transfer Fluid , Mix Fluid , Wash Tips or Pins , Blot Pins , Pause
Pipettor , Finish with Source and Read are all available to drag into a
protocol from the Process Explorer based on the pipettor head type
installed. Wash Reservoir is only available when the Cell Suspension
option is installed.
There are two processes additional to the basic set:
• Mix with TF
• Read with TF
These perform the same functions as Mix Fluid and Read , but are
created as options within a Transfer Fluid step, and are more closely
integrated with the step, for example , a Read with TF is timed
precisely to coincide with compound addition to the read plate.
New protocols always include a
Transfer Fluid step with an associated
Read with TF , but these can be removed or reordered, and of course
additional steps can be dragged across from the Process Explorer .