3.5 Configuring Instrument Settings in Flex Mode
3.5.2 Sensitivity
This setting determines the number of readings that will be performed on each well of
the microplate (readings are averaged and the average reading is displayed).
The default setting is six readings per well for fluorescence and can be adjusted between
Fast and Precise. Increase reading precision by moving the bar toward the right and
increasing the number of readings per well. Decrease the reading precision by moving
the bar to the left and reducing the number of readings per well.
Readings per well range: 1 to 30 readings per well.
Typically the default setting is adequate. Only in wells with very low signal will you get
improved precision by increasing the number of reads per well.
Note:
Increasing the number of reads also increases the total read time.
Sensitivity Settings
The photomultiplier tube (PMT) is a photon detector that detects light, through the use
of photoemission and successive instances of secondary emission, to produce enough
electrons to generate a strong signal.
PMT Settings
Click in the PMT Sensitivity box to display the menu choices. Settings are manual and
limited to High, Medium, or Low.
Set the voltage of the PMT to Low for samples having a higher concentration. Choose
High PMT sensitivity for samples with lower concentration.
Note:
An additional setting, Automatic, is available only in for Endpoint reads and Spectral
scans. Automatic is not available for Kinetic or Flex modes.
FlexStation II Operator’s Manual – Rev. D
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Summary of Contents for FlexStation II
Page 6: ...FlexStation II Operator s Manual Rev D vi ...
Page 92: ...4 Applications 86 FlexStation II Operator s Manual Rev D ...
Page 102: ...5 Service and Maintenance Procedures 96 FlexStation II Operator s Manual Rev D ...
Page 124: ...6 Troubleshooting Procedures 118 FlexStation II Operator s Manual Rev D ...
Page 134: ...7 Appendices 128 FlexStation II Operator s Manual Rev D ...
