FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
42
0112-0127 B
Set Up the Reader and Software Parameters
•
Read microplates in using Flex, Endpoint, Kinetic, Spectrum, or
Well Scan read types
.
Use up to four wavelengths for Flex, Endpoint, Kinetic, and
Well Scan reads.
Perform absorbance and percent transmittance readings in
the 200 nm to 1000 nm range.
Perform fluorescence readings in the 250 nm to 850 nm
range.
Perform luminescence readings in two ways: wavelength
nonspecific (all wavelengths between 360 nm and 630 nm)
or wavelength selectable (250 nm to 850 nm).
Read the whole plate or a subset of microplate wells.
Specify kinetic run times up to 99 hours.
Select your own read intervals for kinetic runs.
Specify the duration for Automix before or between reads.
Automix shakes the microplate at preset intervals, thereby
mixing the contents of each well (highly recommended for
ELISAs and other solid-phase, enzyme-mediated reactions).
Note:
Automix is not intended to be used continuously for
several hours.
•
Use PathCheck technology to normalize the absorbance readings
in each microplate well to a 1 cm pathlength.
•
Design microplate templates to simplify data reduction.
Identify groups of wells with labels of your choice.
Identify individual wells with unique names.
Blank the entire plate, groups, or individual wells.
•
Save reader settings, template formats, and data analysis
parameters as assay protocol files and recall them for later use.
Rapid reader and analysis setup for repeated assays.
Uniform analysis for equivalent microplates.
•
Turn the incubator on or off to control the temperature in the
read plate drawer.
FlexStation_manual.book Page 42 Tuesday, June 25, 2013 10:22 AM
