18
Tilt
User’s Guide
Recommended
Specimen Chambers
The key to successful imaging with the Tilt is an imaging chamber with flat, optically clear
sides to allow an unobstructed path for the lightsheet. Currently there are two commercial
options we routinely use.
Mizar Cubes on #1.5 coverslips.
The Mizar Cube chambers are open-ended modified cuvettes placed on coverslips. They are
custom-made from the same high-quality borosilicate glass as cuvettes and are reusable
and autoclavable. In contrast to manufactured chambers, quality of the side walls is consis-
tent and easy to control for. As long as they are kept clean and free of fingerprints, grease
(used for mounting; see below), etc., the cuvette glass is ideal for lightsheet imaging.
ibidi μ-Slide 2/4/8 Well chambers
The ibidi μ-Slide 2, 4, and 8-well chambered coverslips come pre-sterilized, and are a good
disposable option for general use and cultured cells. Many scientists already comfortable
with culturing cells on chambered slides prefer this option, as only minimal changes in
workflow and sample prep are required for successful lightsheet imaging.
For information on ibidi μ-Slides, visit:
https://ibidi.com/13-chambered-coverslips
Other products in the ibidi μ-Slide line that contain round chambers are NOT suitable for
use with the Tilt.
Note:
Manufacturing of imaging dishes typically does not include quality control for the
side walls. We occasionally find a chamber whose side walls distort the lightsheet. In these
cases, we recommend either scanning for areas less impacted by the imperfections or
mounting the sample in a different chamber altogether.