Min NucleoSpin RNA Clean-up User Manual Download Page 1

Page: 1 / 20

MACHEREY-NAGEL

EN ISO 9001

EN ISO 13485

CERTIFIED

MACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · Germany

France:

MACHEREY-NAGEL EURL

Tel.: +33 388 68 22 68

E-mail: [email protected]

Switzerland:

MACHEREY-NAGEL AG

Tel.: +41 62 388 55 00

E-mail: [email protected]

Germany

and international:

Tel.: +49 24 21 969-0

E-mail: [email protected]

USA:

MACHEREY-NAGEL Inc.

Tel.: +1 484 821 0984

E-mail: [email protected]

RNA clean-up

User manual

NucleoSpin

RNA Clean-up

May 2014 / Rev. 05

A030383

/ 1040.3

Summary of Contents for NucleoSpin RNA Clean-up

Page 1: ...RNA clean up User manual NucleoSpin RNA Clean up May 2014 Rev 05...

Page 2: ...on 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Handling preparation and storage of starting materials 7 2 4 Elution procedures 8 3 Storage conditions and preparation of working solutions...

Page 3: ...A1 10 mL 25 mL 125 mL Wash Buffer RA2 15 mL 15 mL 80 mL Wash Buffer RA3 Concentrate 6 mL 12 mL 3 x 25 mL RNase free H2O 13 mL 13 mL 60 mL NucleoSpin RNA Clean up Columns light blue rings plus Collecti...

Page 4: ...About this user manual It is strongly recommended reading the detailed protocol sections of this user manual if the NucleoSpin RNA Clean up kit is used for the first time Experienced users however ma...

Page 5: ...leoSpin RNA Clean up kits are ideal for the clean up of total RNA from RNA preparations which contain inacceptable amounts of RT PCR inhibitors e g RNA prepared with phenol chloroform based methods Th...

Page 6: ...be handled with the same care as any RNA sample The stability of prepurified RNA samples e g RNA isolated with phenol based protocols depends very much on the performed procedure RNA in biological sa...

Page 7: ...ossible High yield Perform two elution steps with the volume indicated in the individual protocol About 90 100 of bound nucleic acid will be eluted High yield and high concentration Elute with the sta...

Page 8: ...stable up to one year Storage at lower temperatures may cause precipitation of salts Check that 96 100 ethanol is available as additional solution in the lab Before starting any NucleoSpin RNA Clean...

Page 9: ...312 330 403 235 Guanidiniumthiocyanat 30 60 Ethanol 20 35 Achtung Hazard phrases H 226 Flammable liquid and vapour Fl ssigkeit und Dampf entz ndbar H 302 Harmful if swallowed Gesundheitssch dlich bei...

Page 10: ...TINFORMATIONSZENTRUM oder Arzt anrufen P 403 235 Store in a well ventilated place Keep cool K hl an einem gut bel fteten Ort aufbewahren For further information please see Material Safety Data Sheets...

Page 11: ...300 L Buffer RA1 and 300 L of ethanol 96 100 If multiple samples are processed the preparation of a master premix is recommended e g 2 mL Buffer RA1 2 mL 98 ethanol for approximately 6 preparations Pr...

Page 12: ...wash Add 350 L Buffer RA3 to the NucleoSpin RNA Clean up Column Centrifuge for 2 min at 8 000 x g Transfer the NucleoSpin RNA Clean up Column to a nuclease free Collection Tube 1 5 mL supplied Open t...

Page 13: ...g or pipetting up and down After addition of ethanol a stringy precipitate may become visible which will not affect the RNA isolation Be sure to mix thoroughly and apply sample as homogeneous solution...

Page 14: ...the NucleoSpin RNA Clean up Column to a nuclease free Collection Tube 1 5 mL supplied Open the lid of the column and let the membrane dry for 3 min If for any reason the liquid level in the Collection...

Page 15: ...o the silica membrane is only effective in the presence of ethanol Kit storage Store kit components at room temperature Storage at low temperatures may cause salt precipitation Keep bottles tightly cl...

Page 16: ...be reduced 5 20 Store isolated RNA properly Eluted RNA should always be kept on ice for optimal stability since trace contaminations of omnipresent RNases general lab ware fingerprints dust will degr...

Page 17: ...962 20 20 preps NucleoSpin RNA Blood 740200 740200 10 50 10 50 preps preps NucleoSpin miRNA 740971 740971 740971 10 50 250 10 50 250 preps preps preps NucleoSpin RNA Plant 740949 740949 740949 10 50 2...

Page 18: ...o the respective Material Safety Data Sheet of the product MACHEREY NAGEL products shall exclusively be used in an ADEQUATE TEST ENVIRONMENT MACHEREY NAGEL does not assume any responsibility for damag...

Page 19: ...EL components or products MACHEREY NAGEL makes no other warranty of any kind whatsoever and SPECIFICALLY DISCLAIMS AND EXCLUDES ALL OTHER WARRANTIES OF ANY KIND OR NATURE WHATSOEVER DIRECTLY OR INDIRE...

Page 20: ...only MACHEREY NAGEL does not warrant that all applications have been tested in MACHEREY NAGEL laboratories using MACHEREY NAGEL products MACHEREY NAGEL does not warrant the correctness of any of thos...

Search results

Summary of Contents for NucleoSpin RNA Clean-up

Reviews:

Brands by name

Popular brands

Min NucleoSpin RNA Clean-up, User Manual

Search results

Summary of Contents for NucleoSpin RNA Clean-up

Reviews:

Brands by name

Popular brands