Miltenyi Biotec UltraMicroscope II, User Manual

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After the settings have been saved, the alignment tool must not be moved again. It is highly recommended
to block the third button from the left of the jog wheel.
6.4 Adjusting the lateral light sheets on the right-hand side
To adjust the lateral light sheets on the right-hand side, the horizontal focus must not be changed.
1 Click Laser beam adjustment > Right > 3 in the Advanced tab in the Settings 1 window to activate all
laser beams. All light sheets should pass the pinhole in the center of the mask of the alignment tool.
2 Adjust the height of the lateral light sheets. Start with the rear light sheet using the adjustment screw Z2 :
Refer to Figure 6.1 . Use an Allen key to align the light sheet to the pinhole.
3 Repeat the procedure with the front light sheet using the adjustment screw Z2 : Refer to Figure 6.1 .
4 Draw a region of interest over the light sheets passing the pinhole in the live window, and activate the
intensity profile.
5 Check the adjustment by clicking the Brain button under Scan Range in the Settings 2 window. Keep the
cursor on the button, and scroll with the mouse wheel to move the alignment tool up and down.
If the intensity peaks decrease in the same way, the alignment is sufficient.
6 To move back the alignment tool to the reference position, regardless whether the adjustment was
successful, type 0 under Current Position .
7 Press the Move to set position button.
Alternatively use the mouse wheel.
Proceed with adjusting the central light sheet on the left-hand side.
6.5 Adjusting the central light sheet on the left-hand side
1 Click Lightsheet > Select left lightsheet in the Settings 1 window.
2 Click Laser beam adjustment > Left > 1 in the Advanced tab to activate the central beam.
3 Draw a rectangle over the illuminated part and the lines of the light sheet as the region of interest (ROI),
and adjust the contrast.
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