Pellicon
®
3 Cassettes Installation and User Guide
10
* Typical Peracetic acid-containing cold sanitant contain 20-24% Hydrogen Peroxide,
4.6% Peracetic acid, and 8-10% Acetic acid.
** Use milder conditions if effectiveness of agent is still observed. Better membrane
life is expected with lower concentration of caustic and/or at 20-25 °C for regenerated
cellulose membranes with a nominal molecular weight limit of 3 or 5 kDa.
Depyrogenation
If depyrogenation is required, it should only be performed after the system has been
cleaned, sanitized, and flushed. Depyrogenation pressures, flow rates and volumes
are identical to those used during Cleaning. Water for injection should be used during
depyrogenation.
Depyrogenation Conditions and Agents
Membrane
Depyrogenation
Agent
Concentration
Temp
(° C)
pH
Time
(min)
Biomax
®
Phosphoric Acid
0.1M
30 - 50
1
30
NaOH
0.1N
25 - 50
13
30
Ultracel
®
NaOH*
0.1N
25 - 50
13
30
* Use milder conditions if effectiveness of agent is still observed. Better membrane
life is expected with lower concentration of caustic and/or at 20-25 °C for regenerated
cellulose membranes with a nominal molecular weight limit of 3 or 5 kDa.
Integrity Testing
Pellicon
®
3 filter integrity should be
tested on a cleaned and thoroughly
flushed system. The presence of residual
cleaning agents can significantly alter
integrity test results. Integrity testing
should be performed on a water wet
membrane only.
1. Drain the system of water. Drain
the retentate side of the system as
thoroughly as possible.
2. Attach a regulated and filtered air
supply to the feed or retentate
piping, preferably at the high point
of the system.
3. Isolate either the feed or the
retentate piping by closing a valve
or capping the piping if there is no
valve on it (to enable pressurization
of the filter feed channels).
4. Open the permeate line.
Integrity Test setup
