BoneSTATION
MM093-002
– Operator Manual
21
Both the front and back modules have particular accommodations to perfectly host the metal holders. See
next figure for the accommodations.
The two METAL HOLDERS have different labels: one for FIXATIVE and one for DECALCIFIER.
For good laboratory practice and to avoid any mistake, keep the beaker with fixative reagent in the FIXATIVE
metallic holder and the beaker with decalcifier in the DECALCIFIER metallic holder.
3.2. Run Fixation and Decalcification programs
3.2.1. Fixation
This phase is composed by:
1. PRESOAKING: carried out in the HOLDING POSITION and with fixative NOT heated but just
agitated.
2. HEATED FIXATION: carried out in the HOT PLATE, with fixative heated and agitated.
It is possible to skip the pre-soaking step if samples have already been pre-soaked in fixative out of the
BoneSTATION. Otherwise, if samples have not been pre-soaked in fixative yet, include the pre-soaking step
putting the FIXATIVE metal holder with glass containing fixative in the HOLDING POSITION (as shown in
the picture).
