VP-ITC MicroCalorimeter User’s Manual
Now that the cells are filled, it is a good time to check the VP-ITC thermostat temperature and
make sure it is set to the desired run temperature of 30 degrees. If the current thermostat
temperature is not set to 30 degrees, set it now.
Load the Pipette
Please refer to the diagrams below.
•
Place the water titrant into the pipette filling tube.
•
Place the pipette filling tube into the bottom of the pipette stand.
•
Carefully, insert the auto-pipette into the pipette stand.
Caution:
Be careful not to hit the long needle of the injection syringe against any object, as
this could cause the needle to bend and expel some solution from the syringe and result in a
poor first injection for the experiment. If the long needle is bent far enough it may cause a
permanent deformation in the needle making the syringe unusable for future experiments.
•
Select the ITC Controls window. In the
lower right corner you will find the
Pipette Controls button.
•
Click on the
Open Fill Port
button.
The auto-pipette will move the plunger
of the injection syringe till the Teflon
tip is just above the filling port of the
syringe. You should hear a beep when the movement is complete.
!
•
Attach the tube of the
plastic filling syringe
to the
filling port of the injection syringe
.
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