Section 3: Running an ITC Experiment
3.8
Cleaning Syringes.
Cleaning Injection Syringes
•
Disconnect the syringe from the pipette and insert the accessory
plunger into the top of the syringe.
•
Attach the plastic loading syringe tube to the filling port at the top
of the syringe
•
Insert the tip of the injection syringe into the cleaning solution
and draw
≥
2ml of solution through the syringe
•
Empty the plastic loading syringe and repeat the above step at
least two more times.
•
Insert the tip of the injection syringe into distilled water and draw
≥
2ml of solution through the syringe
•
Empty the plastic loading syringe and repeat the above step at
least two more times.
•
Insert the tip of the injection syringe into HPLC Grade methanol
and draw
≥
2ml of solution through the syringe.
•
Empty the plastic loading syringe and reattach it to the port. Remove the accessory plunger.
Reattach the plastic loading syringe.
•
Apply vacuum to the glass end of the injection syringe using the vacuum tube attachment for
approximately 10 minutes to dry the methanol.
If it is necessary, the cleaning brush (included with accessories) may be used to scrub the inside
diameter of the syringe glass. Additionally, there is a cleaning wire included with the ITC
accessories that can be used to clean out the small delivery hole that is located on the very end of
the twisted paddle of the titration syringe. Care should be used to avoid bending the syringe
during this procedure.
Cleaning The Filling Syringes
Cleaning the syringes may be accomplished by various methods. The filling syringes are
generally soaked in a cleaning solution and briefly scrubbed internally with a soft brush. The
filling syringes are then rinsed thoroughly at a sink with distilled water. The syringes are often
dried with HPLC grade methanol and a dry air or vacuum source. They may also be left out to air
dry but this method is not as reliable as forced drying.
Please Note: Do not leave any methanol
residue in the syringe as it would ruin the next experiment.
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