VP-ITC MicroCalorimeter User’s Manual
•
Turn on the ThermoVac vacuum pump. The vacuum will pull the detergent solution from the
beaker, through the cell and into the waste flask.
NOTE: DO NOT ALLOW THE
VOLUME OF FLUID IN THE WASTE FLASK TO BECOME SUCH THAT IT
WOULD RISE UP TO THE LEVEL OF THE SIDE ARM AND BE SUCKED INTO
THE THERMOVAC’S VACUUM PUMP. THIS MAY CAUSE THE PUMP TO
BECOME DAMAGED.
•
Once sufficient detergent solution has passed through the cell, the plastic tubing is removed
from the solution, rinsed free of detergent using a plastic wash bottle, and then placed into
another beaker containing 300 ml or more of water for rinsing.
•
After rinsing with water, remove the plastic tubing from the rinse water and allow time for
the vacuum to drain the fluid out of the plastic tubing, then remove the cleaning apparatus
from the cell.
•
Remove the remaining water from the cell by using a long needle syringe.
We do not recommend drying the cell before filling with your sample solution, but it should be
rinsed twice with the buffer you are using for the experiment. Finally, it should be completely
drained and filled with your sample solution in the manner described earlier.
Because a small amount of the buffer used for final rinsing will adhere to the walls of the cell and
act to dilute your sample solution, you may wish to correct for this by lowering your sample
concentration by 2% if you measured concentration before the sample was introduced into the
cell.
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