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Metrohm 2.947.0010, Manual

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Metrohm 2.947.0010 Manual Download Page 51

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭

5 Operation

947 Professional UV/VIS Detector Vario (2.947.00x0)

￭￭￭￭￭￭￭￭

43

5  Operation

The instrument is operated via the MagIC Net software and the driver
software Metrohm IC Driver for OpenLab.

Additional information on operating MagIC Net can be found in the docu-
ment 

MagIC Net Tutorial

 or in the software's online help.

Additional information on operating the Metrohm IC Driver for OpenLab
can be found in the document 

Tutorial – Metrohm IC Driver 1.0 for Open-

Lab

 or in the online help of the driver software.

Summary of Contents for 2.947.0010

Page 1: ...947 Professional UV VIS Detector Vario 2 947 00x0 Manual 8 0947 8001EN 2020 06 30 ...

Page 2: ......

Page 3: ...Metrohm AG CH 9100 Herisau Switzerland Phone 41 71 353 85 85 Fax 41 71 353 89 01 info metrohm com www metrohm com 947 Professional UV VIS Detector Vario 2 947 00x0 Manual 8 0947 8001EN 2020 06 30 ...

Page 4: ...techcom metrohm com This documentation is protected by copyright All rights reserved This documentation has been prepared with great care However errors can never be entirely ruled out Please send comments regarding possible errors to the address above ...

Page 5: ...nstallation 9 3 1 Setting up the instrument 9 3 1 1 Packaging 9 3 1 2 Checks 9 3 1 3 Location 9 3 2 Setup configurations 9 3 3 Base tray and bottle holder 11 3 3 1 Basic information on base tray and bottle holder 11 3 3 2 Mounting base tray and bottle holder optional 11 3 4 Connecting the flow through cell 16 3 5 Installing the lamps 18 3 5 1 Installing the VIS lamp 20 3 5 2 Installing the UV lamp...

Page 6: ... 4 Replacing the lamps 45 6 4 1 Replacing the VIS lamp 46 6 4 2 Replacing the UV lamp 48 6 5 Cleaning the flow through cell 51 7 Troubleshooting 57 7 1 Problems and their solutions 57 8 Technical specifications 58 8 1 Reference conditions 58 8 2 UV VIS detector 58 8 3 Lamps 60 8 4 Ambient conditions 60 8 5 Dimensions 60 8 6 Housing 61 8 7 Controls 61 8 8 Power connection 61 8 9 Interfaces 61 9 Acc...

Page 7: ...19 Inserting the adjustment ring 25 Figure 20 Tightening the adjustment ring 26 Figure 21 Connecting the UV lamp 26 Figure 22 Setting the integration duration 32 Figure 23 Adjusting the UV lamp 33 Figure 24 Optimized intensity spectrum with UV lamp and VIS lamp 34 Figure 25 Setting the integration duration 35 Figure 26 Adjusting the UV lamp 36 Figure 27 Optimized intensity spectrum with UV lamp 37...

Page 8: ......

Page 9: ...tional readiness MagIC Net controls and monitors the instrument evaluates the measured data and manages it in a data base Additional information on operating MagIC Net can be found in the docu ment MagIC Net Tutorial or in the software s online help 1 2 Product versions The 947 Professional UV VIS Detector Vario is available in the following versions Table 1 Product versions Art no Designation Ver...

Page 10: ...and experience in handling toxic and caustic substances Knowledge regarding the application of fire prevention measures prescribed for laboratories is also mandatory 1 4 About the documentation CAUTION Read through this documentation carefully before putting the instru ment into operation The documentation contains information and warnings which the user must follow in order to ensure safe operati...

Page 11: ...aws attention to possible damage to instruments or instrument parts NOTE This symbol highlights additional information and tips 1 5 Safety instructions 1 5 1 General notes on safety WARNING Operate this instrument only according to the information contained in this documentation This instrument left the factory in a flawless state in terms of technical safety To maintain this state and ensure non ...

Page 12: ...e are no parts inside the housing which can be serviced or replaced by the user Supply voltage WARNING An incorrect supply voltage can damage the instrument Only operate this instrument with a supply voltage specified for it see rear panel of the instrument Protection against electrostatic charges WARNING Electronic components are sensitive to electrostatic charges and can be destroyed by discharg...

Page 13: ...NING All relevant safety measures are to be observed when working with flammable solvents and chemicals Set up the instrument in a well ventilated location e g fume cup board Keep all sources of flame far from the workplace Clean up spilled liquids and solids immediately Follow the safety instructions of the chemical manufacturer 1 5 5 Recycling and disposal This product is covered by European Dir...

Page 14: ...2 1 Front 6 947 Professional UV VIS Detector Vario 2 947 00x0 2 Overview of the instrument 2 1 Front Figure 2 Front Overview 1 Standby indicator 2 Flow through cell ...

Page 15: ...f the UV lamp 4 VIS connection socket For connecting the cable of the VIS lamp 5 UV lamp opening When the instrument is delivered the open ing with the socket cap for the UV lamp is closed 6 VIS lamp opening When the instrument is delivered the open ing with the socket cap for the VIS lamp is closed 7 Lamp cooling element 8 PC connection socket For connecting the USB cable to the PC ...

Page 16: ...t For connecting the LAN cable The LAN connection socket is closed with a blind cover The connector is not functional 10 Analog connection socket Output for analog signals 11 Power socket For connecting the power cord 12 Power switch For switching the instrument on and off I On O Off ...

Page 17: ... free of vibrations Set up the instrument in such a way that it is protected against corrosive atmosphere and contamination by chemi cals Protect the instrument against excessive temperature fluctuations and direct sunlight The distance between the rear of the instrument and the wall must be large enough to ensure air circulation on the cooling plate 3 2 Setup configurations CAUTION Changes to the...

Page 18: ...15 100 An IC system with photometric detection and post column derivatization can be set up in various configurations Figure 4 Setup configurations Notes on the setup configurations If you want to set up the instruments in 2 stacks we recommend you order the following accessories to protect the instruments of the second stack Bottle holder ProfIC 6 2061 100 Base tray with sensor for Professional I...

Page 19: ...Extension Module Vario Or another instrument with the same sized footprint 3 3 2 Mounting base tray and bottle holder optional The base tray and bottle holder come fully assembled on a new ion chro matograph To install an Extension Module on the ion chromatograph remove the bottle holder and put it back on top of the topmost instru ment To install an Extension Module under the ion chromatograph re...

Page 20: ...ng the base tray 1 Tilt the instrument sideways and lay it down flat 2 Loosen the 4 cylinder screws with the 3 mm hex key Remove the cyl inder screws and their washers 3 Remove the base tray Always mount the base tray under the lowermost instrument of the stack Mounting the base tray Prerequisites The instrument is switched off The bottle holder is cleared All of the cable connections on the rear ...

Page 21: ... Stack other instruments in the required order Mount the bottle holder 6 2061 100 onto the topmost instrument on the stack see Mounting the bottle holder page 14 3 3 2 2 Removing mounting the bottle holder Remove the bottle holder if you want to install another instrument onto the IC instrument Removing the bottle holder Prerequisites The instrument is switched off The bottle holder is cleared Dra...

Page 22: ... covering stoppers 2 Loosen the 2 cylinder screws with the 3 mm hex key and remove them 3 Remove the bottle holder Stack other instruments in the required order Mount the bottle holder 6 2061 100 onto the topmost instrument on the stack Mounting the bottle holder Prerequisite The instrument is switched off Accessories 3 mm hex key 6 2621 100 ...

Page 23: ... holder restore all connections that were loos ened at the beginning of the process Restoring the loosened connections 1 Plug in all necessary USB cables 2 Plug in all necessary MSB cables 3 Plug in the power cord 4 Mount the drainage tubing again see manual of the IC instrument A longer section of silicone tubing 6 1816 020 may have to be cut to size and mounted see also the manual for the IC ins...

Page 24: ...ressure in the system Replace leaking parts and connection elements without delay Tighten loose connecting elements Regularly check the leak tightness of the tubings If the instrument is used mainly in unattended operation carry out weekly inspections Use environmentally friendly methods to dispose of escaping liquids To connect the capillaries to the flow through cell proceed as follows Connectin...

Page 25: ...apillary 6 Cylinder screw For the correct alignment of the flow through cell 7 Knurled screws 1 Connecting the detector inlet Unscrew the pressure screw from the IN detector inlet Slide the pressure screw over the detector inlet capillary such that a small part of the capillary is visible at the top Tighten the capillary in the detector inlet using the pressure screw 2 Connecting the detector outl...

Page 26: ...screw NOTICE The detector outlet capillary 6 1831 100 is 1 m long and may not be shortened 3 5 Installing the lamps CAUTION Damage to the lamp Reduced light transmission due to residues on the lamp surface Dam age to the lamp due to burnt on residues on the lamp surface Do not touch the lamp surface with your fingers If the lamp surface is contaminated clean the lamp with alcohol and a lint free c...

Page 27: ...ofessional UV VIS Detector Vario 2 947 00x0 19 Figure 10 Lamp module Overview 1 Knurled screw 2 Adjustment ring 3 UV lamp Deuterium lamp 6 2804 110 4 VIS lamp Halogen lamp 6 2804 100 5 Lamp cooling element 6 VIS lamp holder ...

Page 28: ... removing the lamp Figure 11 VIS lamp 6 2804 100 Overview 1 Plug 2 Lamp socket 3 Groove for the alignment of the lamp 4 Lamp surface Inserting the VIS lamp Prerequisite The instrument is switched off Accessories VIS lamp 6 2804 100 The VIS lamp is not part of the products 2 947 0010 and 2 947 0020 The VIS lamp must be purchased separately 1 Removing the socket cap for the VIS lamp Unscrew the knur...

Page 29: ...sert the lamp into the opening for the VIS lamp 3 6 on the lamp cooling element Align the groove on the lamp socket 11 3 with the positioning pin in the lamp cooling element Push the VIS lamp into the opening for the VIS lamp until it stops Figure 13 Inserting the VIS lamp 3 Fastening the VIS lamp Screw the knurled screw 10 1 tight by hand until the lamp is firmly fixed in the lamp module ...

Page 30: ...S Detector Vario 2 947 00x0 Figure 14 Fastening the VIS lamp 4 Connecting the VIS lamp Plug the plug of the VIS lamp 11 1 into the connection socket VIS 3 4 of the UV VIS detector Tighten the retaining ring of the plug Figure 15 Connecting the VIS lamp ...

Page 31: ...ixed in the lamp module NOTICE Remove the VIS lamp see chapter 6 4 1 page 46 and insert the socket cap if the VIS lamp is not used for a longer period 3 5 2 Installing the UV lamp WARNING Harmful UV radiation Damage to the eyes and skin by UV radiation if the lamp is removed while it is switched on Switch off the instrument before removing the lamp Figure 16 UV lamp 6 2804 110 Overview 1 Plug 2 Te...

Page 32: ...t cap for the UV lamp Loosen the adjustment ring 10 2 Remove the adjustment ring and the socket cap Figure 17 Removing the socket cap for the UV lamp 2 Inserting the UV lamp Hold the UV lamp on the lamp socket 16 3 Insert the lamp into the opening for the UV lamp 3 5 on the lamp cooling element Align the groove on the lamp socket 16 4 with the positioning pin in the lamp cooling element The positi...

Page 33: ...Vario 2 947 00x0 25 Figure 18 Inserting the UV lamp 3 Inserting the adjustment ring Slide the adjustment ring 10 2 over the cable of the UV lamp Figure 19 Inserting the adjustment ring 4 Tightening the adjustment ring Tighten the adjustment ring 10 2 ...

Page 34: ...Detector Vario 2 947 00x0 Figure 20 Tightening the adjustment ring 5 Connecting the UV lamp Plug the plug of the UV lamp 16 1 into the connection socket UV 3 3 of the UV VIS detector Tighten the retaining ring of the plug Figure 21 Connecting the UV lamp ...

Page 35: ...g of the socket cap with the positioning pins in the lamp cooling element Tighten the adjustment ring 10 2 by hand until the socket cap is firmly fixed in the lamp module NOTICE If the 947 Professional UV VIS Detector Vario is operated with the UV lamp and the VIS lamp adjust the UV lamp after start up see chapter 4 1 page 30 NOTICE Remove the UV lamp see chapter 6 4 2 page 48 and insert the socke...

Page 36: ...ctrical potential Risk of injury by touching live components or through moisture on live parts Never open the housing of the instrument while the power cord is still connected Protect live parts e g power supply unit power cord connection sockets against moisture Unplug the power plug immediately if you suspect that moisture has gotten inside the instrument Only personnel who have been issued Metr...

Page 37: ...rio 2 947 00x0 29 Power plug according to customer requirement 6 2122 XX0 min 10 A NOTICE Do not use a not permitted power cord 1 Plugging in the power cord Plug the power cord into the instrument s power socket Connect the power cord to the power grid ...

Page 38: ...ial start up or after replacing a lamp Check the intensity spectrum of the lamps after initial start up or after replacing a lamp and adjust the lamp settings if necessary 4 1 Adjusting the lamp settings NOTICE Adjust the UV lamp after first initial start up or after replacing a lamp see chapter 6 4 2 page 48 and see chapter 6 4 1 page 46 Only adjust the UV lamp if the UV lamp is or the UV lamp an...

Page 39: ...the VIS lamp are installed The instrument is switched on The instrument was configured in MagIC Net see 8 102 8004EN Man ual MagIC Net 3 3 The UV lamp and the VIS lamp have been burning for 30 minutes The flow through cell is clean The flow through cell is rinsed with ultrapure water The flow through cell is free of air bubbles 1 Open the Configuration program part in MagIC Net Double click on 947...

Page 40: ...ration duration 4 Slowly turn the adjustment ring to move the UV lamp in its vertical axis This allows for adjusting the intensity in the range of 400 900 nm Turn the adjustment ring until the maximum intensity is reached Place the cursor on a peak to see better in which direction the inten sity spectrum changes ...

Page 41: ...947 Professional UV VIS Detector Vario 2 947 00x0 33 Figure 23 Adjusting the UV lamp 5 Click on Adjust automatically Optimized values for the integration duration and the VIS intensity level are calculated and set ...

Page 42: ...low the following steps if the instrument is in operation exclu sively with the UV lamp The lamp settings must be adjusted if the lamp was replaced or if checking the intensity spectrum after first start up displays a cut off see chapter 4 2 page 39 Remove the VIS lamp completely see chapter 6 4 1 page 46 Close the opening of the lamp with the socket cap Otherwise the lamp is automati cally switch...

Page 43: ...h cell is free of air bubbles 1 Open the Configuration program part in MagIC Net Double click on 947 UV VIS Detector in the device table The Properties window opens 2 On the Detector tab click on Properties to open the detector settings 3 Set the integration duration manually to 20 0 ms Confirm with Apply Figure 25 Setting the integration duration 4 Slowly turn the adjustment ring to move the UV l...

Page 44: ...Vario 2 947 00x0 Place the cursor on a peak to see better in which direction the inten sity spectrum changes Figure 26 Adjusting the UV lamp 5 Click on Adjust automatically Optimized values for the integration duration and the VIS intensity level are calculated and set ...

Page 45: ...ent is in operation exclu sively with the VIS lamp The lamp settings must be adjusted if the VIS lamp was replaced or if checking the intensity spectrum after first start up displays a cut off see chapter 4 2 page 39 Remove the UV lamp completely see chapter 6 4 2 page 48 Close the opening of the lamp with the socket cap Otherwise the lamp is automati cally switched on as soon as the detector sett...

Page 46: ...ws a cut off see chapter 4 2 page 39 1 Open the Configuration program part in MagIC Net Double click on 947 UV VIS Detector in the device table The Properties window opens 2 On the Detector tab click on Properties to open the detector settings 3 In the detector settings click on Adjust automatically to start the automatic lamp adjustment The lamp settings are made based on a built in algorithm Thi...

Page 47: ...ck on OK The adjusted lamp values are transferred to the instrument The win dow is closed The lamp optimization is complete 4 2 Checking the intensity spectrum Check the intensity spectrum in the following cases After initial start up After replacing a lamp If increased noise or measurement inaccuracies occur after prolonged use Checking the intensity spectrum Prerequisite ...

Page 48: ...n the Work place program part Go to the Manual program part Click on the icon for the 947 UV VIS Detector On the UV lamp tab switch on the UV lamp On the VIS lamp tab switch on the VIS lamp On the Detector tab select the Intensity spectrum First click on Reset baseline and then click on View The lamp s intensity range is monitored and the spectrum is recorded If the intensity spectrum looks simila...

Page 49: ... 2 947 00x0 41 Figure 30 Lamp intensity too high If the intensity spectrum shows an intensity that is too low like the one in figure Lamp intensity too low then the lamp settings must be adjusted see chapter 4 1 page 30 Figure 31 Lamp intensity too low ...

Page 50: ...e Lamp intensity OK the scale for the intensity has a range up to 60 000 To see whether the lamp intensity is too low check if the scale for the intensity has a range up to 60 000 In figure Lamp inten sity too low for example the scale of the intensity only has a range up to 28 000 A lamp intensity that is too low is easier to see in the detector settings as the scale for the intensity here is fix...

Page 51: ...tware Metrohm IC Driver for OpenLab Additional information on operating MagIC Net can be found in the docu ment MagIC Net Tutorial or in the software s online help Additional information on operating the Metrohm IC Driver for OpenLab can be found in the document Tutorial Metrohm IC Driver 1 0 for Open Lab or in the online help of the driver software ...

Page 52: ...has been designed so that liquid is largely prevented from being able to get inside the instrument However unplug the power plug immediately if you suspect that corrosive media have gotten inside the instrument This is the only way to prevent extreme damage to the instrument electronics Contact Metrohm Service immediately WARNING Only trained personnel may open the instrument s housing 6 2 Mainten...

Page 53: ...ent by the door when lifting or moving it 6 4 Replacing the lamps CAUTION Damage to the lamp Reduced light transmission due to residues on the lamp surface Dam age to the lamp due to burnt on residues on the lamp surface Do not touch the lamp surface with your fingers If the lamp surface is contaminated clean the lamp with alcohol and a lint free cloth Make sure the lamp surface is dry before usin...

Page 54: ...on Switch off the instrument before removing the lamp Removing the VIS lamp Prerequisites The instrument is switched off The lamp has cooled down 1 Disconnecting the VIS lamp Loosen the retaining ring on the plug of the VIS lamp Unplug the plug 11 1 from the power socket VIS 3 4 of the UV VIS detector Figure 32 Disconnecting the VIS lamp 2 Loosening the knurled screw Unscrew the knurled screw 10 1...

Page 55: ...ntenance 947 Professional UV VIS Detector Vario 2 947 00x0 47 Figure 33 Loosening the knurled screw 3 Removing the VIS lamp Remove the lamp by pulling the lamp socket 11 2 from the hous ing Figure 34 Removing the VIS lamp ...

Page 56: ...fore removing the lamp After a prolonged burning time the radiation of the UV lamp starts to decrease This can be noticed by an increased noise on the baseline The service life of the lamp is approximately 2 000 operating hours This means that after 2 000 operating hours at a wave length of 250 nm the intensity decreases by 50 at the most Replace the UV lamp if the noise on the baseline becomes to...

Page 57: ...ector Vario 2 947 00x0 49 Figure 35 Disconnecting the UV lamp 2 Loosening the adjustment ring Loosen the adjustment ring 10 2 of the UV lamp Figure 36 Loosening the adjustment ring 3 Removing the adjustment ring Remove the adjustment ring 10 2 of the UV lamp ...

Page 58: ... lamps 50 947 Professional UV VIS Detector Vario 2 947 00x0 Figure 37 Removing the adjustment ring 4 Removing the UV lamp Remove the lamp by pulling the lamp socket 16 3 from the hous ing Figure 38 Removing the UV lamp ...

Page 59: ...sed mainly in unattended operation carry out weekly inspections Use environmentally friendly methods to dispose of escaping liquids Depending on the application hardly visible deposits may form on the len ses with time This may lead to higher absorption and can increase noise on the baseline If the baseline is very noisy and the problem is not caused by other parts of the system the flow through c...

Page 60: ...hrough cell once again with another solvent see Rinsing the flow through cell with another solvent page 52 Rinsing the flow through cell with another solvent A solvent made up of a 1 2 mixture of acetate and isopropanol has shown good results Depending on the application other solvents also prove effi cient Prerequisites Rinsing with methanol was ineffective The inlet capillary is connected to a h...

Page 61: ...ning screws are tightened with a torque wrench This guarantees a pressure stability up to 5 MPa 50 bar Once the flow through cell has been opened and closed again man ually this maximum pressure stability is no longer guaranteed Prerequisites Rinsing the flow through cell with solvent was ineffective Accessories Slotted screwdriver size 5 Seals 6 2764 000 1 Removing the flow through cell Disconnec...

Page 62: ...ving the lens Loosen the retaining screw 39 1 with a slotted screwdriver size 5 and remove it The outer seal is attached to the inside of the regaining screw 39 2 Remove the outer seal Carefully remove the lens 39 3 and the inner seal 39 4 from the measuring cell Put the lens onto a white paper This allows you to see possible deposits There are deposits that only absorb in the UV range These depos...

Page 63: ...ure that the lens lies flat and centered in the recess of the measuring cell Insert a new outer seal 39 2 into the retaining screw 39 1 Reinsert the retaining screw 39 1 and tighten it with a slotted screwdriver size 5 5 Cleaning the second lens Repeat steps 2 to 4 on the opposite side of the cell holder 6 Inserting the flow through cell Insert the flow through cell such that the opening in the to...

Page 64: ...CE In order to fix the flow through cell in the correct position tighten the two knurled screws symmetrically and with constant force Any tilting twisting or canting of the flow through cell influences the light incidence and thus the measuring results Figure 41 Screwing in the knurled screws ...

Page 65: ...se the eluent degasser Flow through cell The lenses are dirty Clean the flow through cell see chapter 6 5 page 51 UV lamp The radiation is too weak Check the intensity spectrum see chapter 4 page 30 If the intensity is too low replace the UV lamp see chapter 6 4 2 page 48 The eluent s absorption is too strong Change the eluent The wavelength is unsuit able Set a different wavelength in MagIC Net T...

Page 66: ... Bandwidth 10 nm Flow through cell Flow through cell PEEK 6 2839 130 installed Measurement duration 1 000 ms Eluent Ultrapure water Flow rate 1 mL min 8 2 UV VIS detector Detector type Diode array detector 1 024 diodes Measuring range Absorption 2 0 2 0 AU Number of channels 2 947 0010 1 measuring channel readable in analog mode Number of channels 2 947 0020 8 measuring channels 1 of them readable...

Page 67: ... nm Measuring interval Data rate for each channel 0 5 10 samples sec Integration duration 15 247 ms Measurement duration 100 2 000 ms Analog output Voltage range 1 0 1 0 V Resolution 0 5 mV Noise 0 5 mV Output impe dance 50 Ω sustained short circuit proof Offset error 1 5 mV Plug 9 pin DSUB plug female Control input Input voltage range 0 5 V 5 V logic or switching contact control possible Impedanc...

Page 68: ...pprox 2 000 h VIS lamp Type Halogen Power con sumption 5 15 W Service life approx 5 000 h 8 4 Ambient conditions Operation Ambient tem perature 5 45 C Humidity 20 80 relative humidity Storage Ambient tem perature 20 70 C Transport Ambient tem perature 40 70 C 8 5 Dimensions Measurements Width 370 mm Height 131 mm Depth 495 mm Weight 10 7 kg ...

Page 69: ...n for fire class UL94V0 CFC free coated IP degree of pro tection IP 20 8 7 Controls Status display LED On off switch On the rear of the instrument 8 8 Power connection Required supply voltage 100 240 V 10 Required fre quency 50 60 Hz 3 Power consump tion 60 VA 8 9 Interfaces USB Input 1 plug type B for connection to PC Further connec tions Analog output control input 9 pin DSUB female ...

Page 70: ...hm com into your Internet browser 2 Enter the article number e g 2 947 00x0 into the search field The search result is displayed 3 Click on the product Detailed information regarding the product is shown on various tabs 4 On the Included parts tab click on Download the PDF The PDF file with the accessories data is created NOTICE Once you have received your new product we recommend download ing the...

Page 71: ...hrough cell Clean 51 Connecting 16 Frequency 61 H Housing 61 Humidity 60 I Install UV lamp 23 VIS lamp 20 Instrument Connect 28 Intensity spectrum Check 39 Interface USB 61 Interfaces Further connections 61 L Lamp settings Adjust 30 M Material 61 P Power connection 28 29 61 Power consumption 61 R Reference conditions 58 Replace UV lamp 48 VIS lamp 46 S Safety instructions 3 Seal 55 Service 4 44 St...

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