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Matrix WellMate, User Manual, Page: 1 / 80

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Summary of Contents for WellMate

Page 1: ......

Page 2: ...Matrix Technologies Corporation 22 Friars Drive Hudson NH 03051 USA Phone 800 345 0206 www matrixtechcorp com Rev 1 6 May 2006 WellMate Stacker User Manual...

Page 3: ...n any form or by any means in whole or in part without the prior written permission of Matrix Technologies Corporation WellMate is a trademark and Matrix Technologies Corp and the Matrix logo are regi...

Page 4: ...Power Cord 23 D SETTING UP AND RUNNING THE INSTRUMENT 24 1 Prime the Tubing Cartridge 24 2 Create Load and Save a Program in Memory 25 3 Run a Program From Memory 27 4 Program Lock Freature Instructi...

Page 5: ...1 Plate Setup 47 2 Chemical Compatibility 51 3 RS 232 ASCII Commands for Remote Device Activation 53 4 WellMate Schematic 64 5 Customer Service 65 WARRANTY 66 PACKING LIST 69 LIST OF REPLACEMENT PART...

Page 6: ...f the WellMate instrument include Height adjustable dispense head that accommodates shallow and deep well plates and blocks High resolution stepper motor technology that allows fast accurate dispensin...

Page 7: ...bing and polypropylene nozzles One each of Standard bore tubing cartridge for use with 96 well shallow or deep well microplates Nozzle orifice ID 0 023 in 0 58 mm Dispenses volumes 20 2000 l Applicati...

Page 8: ...te Stacker unit which contains Power Cord Connection Cable Priming trough with tubing and hardware Chimney support brackets and hardware Stacker base 2 breakaway washers You must provide the following...

Page 9: ...s with the following components Disposable Tubing Cartidges Keypad Removable Plate Carriage Stage w Spacer Guide Waste Fluid Vessel Plate type Selector Nozzle Head Power Cord Removable Plate Carriage...

Page 10: ...otograph however two disposable tubing cartridges are included in the initial system package one standard bore tubing and one small bore tubing The WellMate Stacker package comes with the following co...

Page 11: ...page 24 Key and Display Description clockwise from top left LED Display Displays volume selected channel program number and error message codes SELECT When PATTERN mode is selected allows you to cycl...

Page 12: ...e allows you to increase or decrease dispense volume PATTERN mode allows you to select or deselect individual channels for dispense MEMORY mode allows you to choose a program number for a program VOL...

Page 13: ...ess Optical plate sensors Allows the stacker unit to run until the stacker chimney is empty then stops the run Stacker chimneys Tall chimneys accommodate up to 50 standard height plates short chimneys...

Page 14: ...xtensions of power cord and waste fluid tubing Weight 24 lbs 10 89 kg Description 8 channel pre sterilized standard bore disposable tubing cartridge Inner diameter nozzle orifice 0 023 in 0 58 mm Reco...

Page 15: ...ate filling Enhanced dispense precision Dispensing of high vapor pressure fluids Dispense accuracy precision 2 0 or 0 25 l 20 l Precision limit 1000 96 well microplates processed with 100 l dispense v...

Page 16: ...servoir two chimney sizes available 50 or 25 plates 501 30005 or 501 30006 Power Supply Input Voltage 100V AC or 240V AC Consumption 50 60 Hz 40VA Measurements Width 31 06 in 78 89 cm Depth 12 65 in 3...

Page 17: ...ean the keypad with bleach solutions or other solvents To clean the unit first remove the tubing assembly from the instrument Then use a bleach solution 2 or an aqueous based cleaner to clean surfaces...

Page 18: ...set make sure that the adjustment screws are accessible To do this have the screws on the top half when you lay out the tube set so the adjustment screws will be toward the middle of the pump when pre...

Page 19: ...14 Configuring the Device 3 When installing the tubing place the left hand tube holder onto the arm first Then place the other holder into position Rotate the arm so the tubing is set into position...

Page 20: ...15 Configuring the Device 4 Push the cover on the pump mechanism to the closed position 5 Insert the nozzle tip head as shown in the following Adjustment screws are towards middle of pump...

Page 21: ...that it is pushed in completely If it is still not level slide the stage under the nozzle tips and put a weight on the left your left side of the stage Then use a flathead screwdriver to adjust the le...

Page 22: ...osts extending upward from the flange on the left side of the plate carriage and drop the waste fluid vessel over the posts Place the open end of the tube into a receptacle that sits below the surface...

Page 23: ...se set up a sample plate as follows 1 Set the plate type by moving the plate type slide in toward the machine for 384 well plates and out and away from the machine for 96 well plates Move plate type s...

Page 24: ...ont to back position by turning the knob on the Y axis arm Note Use the 1 5 mm Allen wrench that is provided to tighten the setscrew after Y axis adjustment 4 Raise the nozzle tips Put the nozzle heig...

Page 25: ...e back of the nozzle height reference scale and attach it to the instrument to the left of the plate type lever When you have set the tips for a particular plate to the height you want to dispense fro...

Page 26: ...owing procedure to install the WellMate Stacker Base Unit 1 Remove the plate stage and fluid reservoir from the WellMate Dispenser 2 Place the WellMate instrument onto the stacker base unit in the spa...

Page 27: ...re in place but not completely tightened put the stacker chimney in place While it is in place tighten the hex screws 5 Attach the tubing to the fluid reservoir 6 Insert the fluid reservoir and screw...

Page 28: ...llowing figure while the WellMate uses the COM 2 port 6 Attach the Power Cord Plug the power cord into the power cord receptacle on the back of the WellMate unit The stacker is now installed Go to pag...

Page 29: ...ange the dispensing speed setting This section describes those priming programming operation and adjustment tasks 1 Prime the Tubing Cartridge Whenever you change the tubing cartridge you will need to...

Page 30: ...head 2 Create Load and Save a Program in Memory You can construct and save up to 18 programs 9 for 6 to 96 well plates and 9 for 384 well plates that allow you to easily adjust dispense volume and co...

Page 31: ...to increase or decrease volume the current volume appears in the LED display Note To increase the dispense volume in increments of 200 l press RESET 3 Select the dispense pattern which plate columns...

Page 32: ...1 9 appear one at a time in the display If you are using a 384 well plate numbers 11 19 appear in the display When the program number you want to use appears in the display press SELECT LoAd appears...

Page 33: ...ess START The program begins running 10 Press STOP if you need to stop the program Press START if you want to restart from the point where the program stopped press RESET to restart the program from t...

Page 34: ...ram number you want to use appears in the display press SELECT LoAd appears on the display d Press SELECT again save appears on the display e Press START PASS will appear on the display f Press the PR...

Page 35: ...ECT again PASS appears on the display if the program is locked c Press PRIME button appears on the display d Use the UP or DOWN arrows to input the 4 digit password for that program Pressing PRIME to...

Page 36: ...Start key 96 should show on WellMate display Verify that the 96 plate type light is illuminated e Press the Start key The plate shuttle should move until column 7 of the plate is under the dispense n...

Page 37: ...pense nozzle arm and verify that the 384 indicator light on the WellMate is illuminated b Press the START button The plate shuttle should move until column 13 row set 1 of the plate is under the dispe...

Page 38: ...the plate type Set the plate type by moving the plate type slide in toward the machine for 384 well plates and out away from the machine for 96 well plates Verify that the plate type LED for your pla...

Page 39: ...ch column 6 Press the PRIME key to draw the agent into the tubes and ensure that liquid flows freely through all tubing in a continuous flow 7 When you are finished with the dispensing operations pres...

Page 40: ...the instrument 3 Press START Verify that the plate type LED on the display and the plate selector are both set to plate type 96 4 Press START The stage will move automatically so that column 7 is unde...

Page 41: ...1 1 Press the PRIME and EMPTY keys simultaneously and turn on the instrument 2 Press SELECT to cycle through the three pump speed choices as they appear in the LED Display The choices are S 1 High sp...

Page 42: ...s feature is useful when dispensing fluids that may form droplets at the end of the tip nozzles or when dispensing viscous fluids To use this program mode 1 While holding down the MODE button press th...

Page 43: ...s Timed Prime PT The prime duration is set in real time seconds from 1 9999 a Select the time for example 20 for 20 seconds using the UP or DOWN arrows b Press the SELECT button to save the time When...

Page 44: ...tend the life of your tubing assembly refer to the Helpful Hints section on page 43 1 Clean the Tubing Assembly Table 6 Care and Maintenance of Tubing Assembly Task How Often Procedure Rinse tubing Af...

Page 45: ...the dispensed volume of each channel during the calibration sequence Zero the scale between readings Note There is a purge before each dispense cycle of the WellMate It is important NOT to collect th...

Page 46: ...for the ten shots dispensed or 0 8 mg dispense 6 Repeat the previous step until three consecutive weights are within the gravimetric range stated above Do this with all eight channels Record results...

Page 47: ...te the results If any CVs for ANY row are greater than the specifications for the tube set the tube set will need to be adjusted for that channel Adjust the calibration by turning the adjustment screw...

Page 48: ...the tubes were not fully purged general wear over time Continuous use of a tube set may also cause droplets to form outside the tips You can reduce this problem by periodically checking the tips and g...

Page 49: ...ector arm was likely bumped into the wrong position Press STOP to cancel the alarm Check the plate type setting and reset it if necessary Press RESET to return to operation status E004 Stage does not...

Page 50: ...perly engaged on the two pins at the back of the unit Use a flathead screwdriver to adjust the nylon screws to level the carriage Also the mechanism on the bottom of the stage might not be activating...

Page 51: ...he stacker unit off move the plate shuttle to access the right plate stage and make sure the breakaway washer and screw are set correctly E104 Sensor is reporting that the left plate stage is not at t...

Page 52: ...47 H Appendices 1 Plate Setup The following graphics and tables illustrate the plate setup for 6 12 24 and 48 well plates 6 Well Plate Setup Troubleshooting...

Page 53: ...48 Appendices 12 Well Plate Setup...

Page 54: ...49 Appendices 24 Well Plate Setup...

Page 55: ...50 Appendices 48 Well Plate Setup...

Page 56: ...patibility Chemical Conc Temp C Result Acetone 20 56 Benzyl alcohol 20 100 Chloroform 20 Diacetone alcohol 20 168 Dimethyl formamide 100 DMSO 100 20 Acetic acid conc 20 100 NOTES No limit to use of tu...

Page 57: ...ic acid 10 20 Detergent solution 1 20 Hydrogen peroxide 10 20 30 NOTES No limit on use Caution not appropriate for some uses Do not use 1 Two grades of ELASTOCIL tubing were tested with each chemical...

Page 58: ...provide a Dsub connector RS 232 cable to order from Matrix use item number 501 30019 and a Windows computer Connect the cable from the COM 1 port on the back of the WellMate instrument to your comput...

Page 59: ...nored as noise 4 Transmission Protocol c Transmission error with repeated no response is to be judged by the transmitting side d PC does not return ACK against R transmission from WellMate G G TXD TXD...

Page 60: ...red ACK Positive response BSR Reads the Back Step Volume Returns a four character string 0000 through 9999 Volume is in 100 nanoliter units BSW xxxx Sets the Back Step Volume Must be a four character...

Page 61: ...y This action is to be done prior to every plate filling sequence It ensures pump head is at Start position P1 Pump action Start dispensing Continue pump action until P3 command is executed P2 Pump ac...

Page 62: ...nternal pump count Returns a seven character string 0000000 through 2684354 PCW xxxxxxx Sets the internal pump count Must be a seven character string 0000000 through 2684354 PPR Returns the pump pause...

Page 63: ...uery What is column well position Queries for ASCII packet that identifies the column well location Only effective for XW command that last moved X axis R RESET Initializing each axis Used to reset al...

Page 64: ...racter is a sign last three are decimal digits W xxx Possible values 002 999 Wait time xxx 10 mSEC Min delay time 002 20mSEC Wait time time that the system waits between executing column dispenses P1...

Page 65: ...osition Command X0 cannot execute until stage is at home Also error occurs if home sensor or stage motor is not working E02 Stage is not at start left most position Command X1 cannot execute until sta...

Page 66: ...smitted command from PC to WellMate instrument Rx Response from WellMate instrument to PC Each ACK is an acknowledgement that a command has been received Query Is stage at home position Tx QA Rx ACK R...

Page 67: ...as sent is error E99 Tx C Rx ACK Rx OK Tx ACK Tx QA Rx ACK Rx E99 Tx ACK COM open and COM close commands sent Tx O Rx ACK Rx 2 1 A1 B0 C1 D0 XW00 000 Tx ACK Rx OK Tx ACK Tx C Rx ACK Rx OK Tx ACK COM o...

Page 68: ...P 04000XW11W002P 04000XW12W002 P 04000XW13W002P 04000XW14W002 P 04000XW15W002P 04000XW16W002 P 04000XW17W002P 04000XW18W002 P 04000XW19W002P 04000XW20W002 P 04000XW21W002P 04000XW22W002 P 04000XW23W00...

Page 69: ...S DRAWING IS THE SOLE PROPERTY OF MATRIX TECHNOLOGIES CORP ANY DISTRIBUTION OF THIS DRAWING DISCLOSURE OR USE OF CONFIDENTIAL INFORMATION CONTAINED HEREIN OR REPRODUCTION IN PART OR WHOLE WITHOUT THE...

Page 70: ...ided by a distributor not listed here contact that distributor If you need to return the instrument for any reason see the instructions provided in the Warranty on page 66 of this manual Corporate Hea...

Page 71: ...ially when the repair may be technically sophisticated or difficult If you need to return the instrument or parts of the instrument to your equipment provider for any reason follow this procedure 1 Co...

Page 72: ...e list on the Matrix Technologies website at www matrixtechcorp com or contact your equipment provider 4 Ship the returned material by 2 day air service The contents of this manual may change as new f...

Page 73: ...uids and beads To order replacement 5 pack use item no 201 30001 Small bore tubing cartridge for use with 96 and 384 well shallow or deep well microplates Nozzle orifice ID 0 015in 0 38 mm Dispenses v...

Page 74: ...ate Stacker Base Unit Each 501 30005 Removable Stacker Chimney Tall 2 required Each 501 30006 Removable Stacker Chimney Short 2 required Each 201 30001 8 channel standard bore disposable tubing cartri...

Page 75: ...chip 3 21 error 44 C001 45 command wrong format 60 dispensing between wells 45 E001 44 E002 44 E003 44 E005 45 E01 60 E02 60 E03 60 E04 60 E99 60 plate type change 44 plate type change RS 232 comm 53...

Page 76: ...e 8 safety 11 schematics 64 serial port communication specifications 54 60 small bore tubing 2 small bore tubing specifications 10 specifications 9 instrument 4 small bore tubing cartridge 10 stacker...

Page 77: ...ications for 9 wait time between RS 232 commands 59 warnings 12 warranty 66 waste reservoir 8 17 21 waste fluid vessel attaching 17 wells plate selecting for fill 26 X axis stage position 25 31 34 Y a...

Page 78: ...73 Notes...

Page 79: ...74 Notes...

Page 80: ...8 channel PTFE coated stainless steel tip cartridge reusable pre sterilized Each 4954 Automation friendly universal polystyrene lid clear sterile for 96 384 well plates 80 case 4955 Automation friendl...

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