System descri pti on
24
General introduction
MicroCal PEAQ-ITC provides detailed insight into binding energetics.
The system has a sample cell and provides direct measurement of the heat absorbed or
evolved as a result of mixing precise amounts of reactants. The sample and reference cells
are made from Hastelloy™, a highly inert material.
Data analysis is performed using MicroCal PEAQ-ITC Analysis Software, wherein the user
obtains the stoichiometry (n), dissociation constant (K
D
), and enthalpy (ΔH) of the inter-
action. The MicroCal PEAQ-ITC Analysis Software can also be used to fit more com-
plicated models.