Macherey-Nagel NucleoSpin RNA Clean-up XS User Manual Download Page 15

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MACHEREY-NAGEL – 03/2021, Rev. 05

RNA clean up XS

6.2 Ordering information

Product

REF

Pack of

NucleoSpin

RNA Clean-up XS

740903.10
740903.50
740903.250

10 preps
50 preps
250 preps

NucleoSpin

RNA XS

740902.10
740902.50
740902.250

10 preps
50 preps
250 preps

NucleoSpin

RNA

740955.20
740955.50
740955.250

20 preps
50 preps
250 preps

NucleoSpin

RNA Midi

740962.20

20 preps

NucleoSpin

RNA/Protein

740933.10
740933.50
740933.250

10 preps
50 preps
250 preps

NucleoSpin

TriPrep

740966.10
740966.50
740966.250

10 preps
50 preps
250 preps

NucleoSpin

RNA Clean-up

740948.10
740948.50
740948.250

10 preps
50 preps
250 preps

NucleoSpin

miRNA

740971.10
740971.50
740971.250

10 preps
50 preps
250 preps

NucleoSpin

RNA Blood

740200.10
740200.50

10 preps
50 preps

NucleoSpin

RNA Plant

740949.10
740949.50
740949.250

10 preps
50 preps
250 preps

NucleoSpin

FFPE RNA

740969.10
740969.50
740969.250

10 preps
50 preps
250 preps

NucleoSpin

RNA/DNA Buffer Set

740944

Suitable for 100 preps

rDNase Set

740963

1 set

NucleoSpin

Filters

740606

Collection Tubes (2 mL)

740600

1000

Summary of Contents for NucleoSpin RNA Clean-up XS

Page 1: ...www mn net com www mn net com Bioanalysis MACHEREY NAGEL User manual March 2021 Rev 05 n NucleoSpin RNA Clean up XS RNA clean up...

Page 2: ...tions 6 2 3 Handling preparation and storage of starting materials 7 2 4 Elution procedures 8 2 5 Stability of isolated RNA 8 3 Storage conditions and preparation of working solutions 9 4 Safety instr...

Page 3: ...up Buffer RCU Concentrate 5 mL 5 mL 5 x 5 mL Wash Buffer RA3 Concentrate 6 mL 12 mL 50 mL RNase free H2O 13 mL 13 mL 13 mL NucleoSpin RNA Clean up XS Binding Columns light blue rings plus Collection...

Page 4: ...es goggles 1 3 About this user manual It is strongly recommended reading the detailed protocol sections of this user manual if the NucleoSpin RNA Clean up XS kit is used for the first time Experienced...

Page 5: ...sample material covers nanogramm to microgramm amounts of prepurified RNA e g phenol purified RNA and RNA from reaction mixtures e g DNase treated samples The innovative column design with a funnel s...

Page 6: ...260 A280 1 9 2 1 Elution volume 5 30 L Preparation time Approx 20 min 6 preps Binding capacity 110 g 2 3 Handling preparation and storage of starting materials RNA intended to be used as sample for th...

Page 7: ...en even requires a subsequent concentration to be suitable for the desired application In contrast to standard kits NucleoSpin RNA Clean up XS allows an efficient elution in a very small volume result...

Page 8: ...tarting any NucleoSpin RNA Clean up protocol prepare the following Clean up Buffer RCU Add the indicated volume of 96 100 ethanol to the Clean up Buffer RCU Concentrate See table below or bottle label...

Page 9: ...cts Sch dlich f r Wasserorganismen mit langfristiger Wirkung Precaution phrases P 264W Wash with water thoroughly after handling Nach Gebrauch mit Wasser gr ndlich waschen P 273 Avoid release to the e...

Page 10: ...necessary spin down gently approx 1 s at 1 000 x g to clean the lid 1 vol RCU Mix 2 x 5 s 3 Bind RNA Take one NucleoSpin RNA XS Column light blue ring placed in a Collection Tube for each preparation...

Page 11: ...min at 11 000 x g to dry the membrane Place the column into a nuclease free Collection Tube 1 5 mL supplied If for any reason the liquid level in the Collection Tube has reached the NucleoSpin RNA XS...

Page 12: ...ins high quality recombinant RNase free DNase rDNase and reaction buffer It is optimized for a highly efficient digestion in order to remove even traces of contaminating DNA 1 Digest DNA reaction setu...

Page 13: ...r RCU Concentrate as described in section 3 Store kit components at room temperature 18 25 C Storage at lower temperatures may cause salt precipitation If precipitation occurs incubate the bottle for...

Page 14: ...ral lab ware fingerprints dust will degrade the isolated RNA For short term storage freeze at 20 C for long term storage freeze at 70 C Higher RNA yield than theoretically possible If performing clean...

Page 15: ...preps 50 preps 250 preps NucleoSpin TriPrep 740966 10 740966 50 740966 250 10 preps 50 preps 250 preps NucleoSpin RNA Clean up 740948 10 740948 50 740948 250 10 preps 50 preps 250 preps NucleoSpin miR...

Page 16: ...riginal MACHEREY NAGEL product leaflets MACHEREY NAGEL products are intended for GENERAL LABORATORY USE ONLY MACHEREY NAGEL products are suited for QUALIFIED PERSONNEL ONLY MACHEREY NAGEL products sha...

Page 17: ...IES OF ANY KIND OR NATURE WHATSOEVER DIRECTLY OR INDIRECTLY EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION AS TO THE SUITABILITY REPRODUCTIVITY DURABILITY FITNESS FOR A PARTICULAR PURPOSE OR USE MERC...

Page 18: ...ademark of Thermo Fisher Scientific SYBR is a registered trademark of Molecular Probes Inc All used names and denotations can be brands trademarks or registered labels of their respective owner also i...

Page 19: ...9 0 info mn net com CH Tel 41 62 388 55 00 sales ch mn net com FR Tel 33 388 68 22 68 sales fr mn net com US Tel 1 484 821 0984 sales us mn net com MACHEREY NAGEL GmbH Co KG Neumann Neander Str 6 8 52...

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