Luminex 200 System User Manual
10
Accuracy and Precision
•
Sample uptake volume: ± 5%
•
Classification of xMAP microspheres: > 80%
•
Misclassification of xMAP microspheres:
2 % - may vary by
xMAP microsphere product lines. Refer to the specific product
information sheet for further details.
•
Temperature control: 0°C to + 2°C of target
•
Internal sample carry over: < 0.9%
•
Soluble background fluorescence emission at 575 nm
automatically subtracted from fluorescence intensity values
Sensitivity
•
Detect 1000 fluorochromes phycoerythrin (PE) per xMAP
microsphere
•
Reporter channel dynamic range: 3.5 decades of detection
Capacity
The specifications below reflect minimum capacity values:
•
Analyze multiple 96-well plates per batch
•
Analyze multiple assay templates per plate
•
Distinguish a minimum of 1 to a maximum of 100 unique xMAP
microsphere sets in a single sample
•
Detect and distinguish surface reporter fluorescence emissions at
575 nm on the surface of 1-100 unique xMAP microspheres sets
in a single sample
•
Sample core: 15-20 µm core at 1 µL/sec. sample inject rate
•
Maintain samples at a constant temperature from 35°C to 55°C
(95°F to 131°F)
•
Automatic sampling from a 96-well plate
•
Start sampling from any well position
•
Sheath container and waste container hold enough volume to run
up to two 96-well plates between refills
•
Microtiter plates with 96 wells must be compatible with the
Luminex XYP instrument plate holder. The following microtiter
plate types are compatible with the Luminex XYP instrument
plate holder: flatbottom, conical, round, filter bottom, half plates,
overall height no more than 0.75” (19 mm), any color.
•
Microtiter plates with 96 wells must be compatible with
Luminex XYP instrument heater block temperature from 35°C to
55°C (95°F to 131°F) when performing heated assays and using
the heater block.
Summary of Contents for Luminex 200
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