Lorrca MaxSis User Manual Download

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LORRCA MAXSIS

USER MANUAL

Version 5.04 MRN-231-EN

Master Registration Number: MRN-231-EN

Summary of Contents for MaxSis

Page 1: ...LORRCA MAXSIS USER MANUAL Version 5 04 MRN 231 EN Master Registration Number MRN 231 EN...

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Page 3: ...com Internet http www rrmechatronics com Postal address P O Box 225 1620 AE Hoorn The Netherlands Office address De Corantijn 13 1689 AN Zwaag The Netherlands Copyright RR Mechatronics Manufacturing...

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Page 5: ...erational instructions Maintenance Mains specification changed Oxygenscan procedure changed Cleaning instructions changed Document History added as appendix only latest changes in introduction H Schav...

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Page 7: ...duction RBC aggregation 26 3 3 4 5 Introduction Oxygenscan 27 4 SAFETY 28 4 1 General safety rules 28 4 2 Electrical safety 30 4 3 Laser and mechanical safety 31 4 4 Bio Safety bob assembly 32 4 5 Saf...

Page 8: ...options 62 6 4 4 2 Syllectogram options 63 6 4 4 3 Iteration parameters setting 64 6 4 4 4 FSAR options 66 6 4 5 Osmoscan options 66 6 4 6 Oxygenscan options 67 6 4 6 1 pO2 Steps 68 6 4 7 Screen commu...

Page 9: ...7 2 4 Oxygenscan whole blood volume calculation 104 7 3 Performing Osmoscan 104 7 3 1 Preparing a blood sample 105 7 3 2 Place sample tube Osmoscan screen 106 7 3 3 Adjust video camera Osmoscan scree...

Page 10: ...r disposal 145 8 REPORTING 146 8 1 Deformability Report 146 8 2 Cell Membrane Stability report page 1 of 3 147 8 3 Cell Membrane Stability report page 2 of 3 148 8 4 Cell Membrane Stability report pag...

Page 11: ...0 10 6 Preparation for long time inactivity 184 11 APPENDIX FOR LORRCA MAXSIS 185 11 1 Preparation for transport 186 11 2 Instructions for packing 187 11 3 Part 1 187 11 4 Part 2a 188 11 5 Part 2b 189...

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Page 13: ...responsibility to link and disseminate the knowledge gained in the worldwide community of researchers and clinicians Our people are passionate about the invention and development of highly precise an...

Page 14: ...partment RR Mechatronics retains the right to make any changes to the contents without any previous warning Check our website for the latest version of this manual http www rrmechatronics com For info...

Page 15: ...a list Emphasis Use to emphasise the importance of a point or for variable expressions such as parameters In Software descriptions Tabs like SCAN SETTING Text buttons like Stop Save Toggle buttons lik...

Page 16: ...ction of the instrument Warning sign to prevent personal injury due to electrical shocks Warning sign to prevent personal injury due to rotating parts Warning sign to prevent personal injury due to bi...

Page 17: ...sists of the instrument and a control PC with printer You can find detailed drawings of the main components in LORC109000 Desk arrangement Main components 1 Computer and display 2 BOB 3 CUP 4 Projecti...

Page 18: ...hatronics instrument identification plate an illustration of this is shown below with explanation The instrument identification plate is located on the right hand side of the rear panel of the instrum...

Page 19: ...V 1 6 Amp Slow blow 110V 3 15 Amp Power consumption Standby 100 VA Maximum 150 VA Heat output Standby 100 Watt Full operation 150 Watt Dimensions Width 470 mm Height with closed lid 400 mm Height with...

Page 20: ...Inner Radius 16 01 0 005 mm Surface roughness Ra 0 026 m Material PMMA BOB Outer Radius 15 65 0 005 mm Surface roughness Ra 0 026 m Material PMMA Ambient temperature 4 C Drive Minimal cup speed 0 03...

Page 21: ...Instrument description Lorrca Maxsis User Manual Page 19 Version 5 04 MRN 231 EN Duration maximum 10 minutes Dilution with full blood 200 1 20 VC elongation measurement 5 at 3Pa...

Page 22: ...of deformation curve Cell Stability test Cell Membrane Stability Red Blood Cell Deformability under various osmotic conditions Osmoscan Osmotic resistance Red Blood Cell Aggregation Cell aggregation k...

Page 23: ...rmability measurement Easy sample changing 3 mW laser diode in shielded setup for maximum user safety Adjustable shear rates from 0 800 s 1 On line data analyses and storage via digital camera system...

Page 24: ...r stress Pascal 1 Pa 10 Dynes cm2 mPa s Dynamic viscosity 1 mPa s 1 cP centi Poise mm Distance millimeter C Temperature degrees Centigrade Elongation parameters A Vertical axis of diffraction pattern...

Page 25: ...on au result of curve fit Tr RBC shape recovery time constant s result of curve fit If RBC rouleaux formation contribution au result of curve fit Tf RBC rouleaux formation time constant s result of cu...

Page 26: ...ppendix Additional Information 3 3 4 1 Principle of operation Lorrca MaxSis In order to evaluate the flowing capacity of blood in different parts of the body it is important to have a technique availa...

Page 27: ...ne Stability Test Cell aggregation Various aggregation indices are determined in whole blood by means of reflection measurements The software instructs the operator when to insert the test suspension...

Page 28: ...reatment exposure to shear stress and glutaraldehyde of normal RBC demonstrating the expected effects 3 3 4 3 Introduction RBC elongation RBC elongation program quantifies the stress dependent Deforma...

Page 29: ...ongation index of the RBCs in shear rate in SC RBCs Sickle Cell patients Red Blood Cells Oxygenscan read out parameters are EImax which represents RBC deformability at normoxia EImin represents deform...

Page 30: ...impaired the instrument must be made inoperative and be secured against any unintended operation The matter should then be referred to qualified technicians Safety protection is likely to be impaired...

Page 31: ...local regulations Check your local environment rules about discharging the waste Inside the instrument hazardous voltages reside Never open the Lorrca MaxSis Leave servicing to qualified service pers...

Page 32: ...he original delivered main power cord or exact equivalent The protective covers have the sign WARNING Ensure that covers are closed during normal use Always connect to grounded wall sockets Set the ma...

Page 33: ...od is open avoid direct eye exposure The corresponding position of each label on the product indicated Label on the outside of the instrument This instrument is equipped with a Class 3R laser Avoid di...

Page 34: ...y to the retina Be aware of beam reflections off glass and shiny surfaces Depending on the surface the reflected beam could be about as strong and as focused as a direct beam The Nominal Ocular Hazard...

Page 35: ...tected by the individual Any source of gas contributing to change in atmosphere should be investigated and assessment carried out to determine whether environment is safe for continuation of work 11 1...

Page 36: ...onduct procedures likely to create splashes sprays or aerosols within a biological safety cabinet that is certified annually Decontaminate work surfaces at least daily Decontaminate waste materials be...

Page 37: ...and occupational hazards may result in situations currently considered safe to be reclassified as having risk Universal Precautions describes a set of procedures for dealing with subjects based on th...

Page 38: ...ht into or consumed inside a blood laboratory at any time Accident reporting All accidents and injuries must be reported within 24 hours to the technician in Charge to the Departmental Joint Health an...

Page 39: ...tallation requirements Only when pO2 Scan will be used Laboratory Integrated Dry Nitrogen Gas Supply NOT part of accessory set consisting of Laboratory dry N2 purge system wall socket connection Low p...

Page 40: ...ressure reduction valve flow regulator FOR EXAMPLE Alwell type EN ISO 2503 as depicted set to 9 ltr min Output pressure 1 bar max RU3 compatible Low pressure gas tube connection between pressure reduc...

Page 41: ...gas tube connection For safety purposes Oxygen meter 5 2 Transport When lifting the packaged instrument take the following information into account The total weight of the instrument is 75 kg includi...

Page 42: ...ions For good operation the CUP BOB bottles keyboard and mouse should be easily seen and easy to reach It is necessary that the locking protection covers are fully accessible for adjustment and repair...

Page 43: ...nection an IEC Lock PC 1063 120V US power cord can be delivered The pO2scan comes along with an additional power strip to make sure both Lorrca pO2scan can be powered The power strip is equipped with...

Page 44: ...er and monitor out of the packaging and remove their boxes accordingly Place it on the table 5 Connect the Laptop and Printer cables according the laptop and Printer installation instructions 6 Take t...

Page 45: ...D plate Factory default is 230VAC See section Replace the main fuses on page 170 11 Fill the bottle de ionized water Fill the bottles for the Osmoscan 12 Place the bottles and connect them according t...

Page 46: ...t the left side of the laptop For Oxygenscan only Connect the instrument to a N2 supply with use of the parts from the accessory set and advised additional material see Pre installation requirements o...

Page 47: ...you have to carry out the following checks and procedures Check that there are no strange objects tools for example in the instrument Switch ON the instrument Start up the PC Choose LoRRca software p...

Page 48: ...n screen holder itself can also be adjusted equipped with slotted holes Tighten the screws of the projection screen holder Not OK Driver failure Update the camera driver See Camera driver installation...

Page 49: ...nctionality Both the elongation and aggregation program feature a check window that can be used to test the hardware functions individually LoRRca status LoRRca IO Camera The adjustments made with thi...

Page 50: ...ck scatter intensity is acquired Intensity au Back scatter intensity arbitrary units should be within the range 0 100 au during measurements when blood is inserted BOB temperature Current BOB temperat...

Page 51: ...On Flash Modulated 3 Off Motor mode Control to select one of the following motor modes 1 Run until in home position 2 Run continuous If in the home position the motor can be re activated by changing...

Page 52: ...Camera With this test the laser is turned ON continuous and the motor speed is maintained as set in any of the other tabs The image shows a coloured version of the diffraction pattern see screen The...

Page 53: ...program and controlled by mouse pointer The following type of measurements can be selected from the main screen Default screen is the Main screen on page 52 Deformability curve determination screen o...

Page 54: ...Lorrca MaxSis Program Page 52 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 6 1 Main screen 6 2 Main screen settings...

Page 55: ...Connection 1 Serial output comport ASRL1 INSTR By default 2 Baud rate Selectable 1200 4800 9600 38400 and many more default is set on 115200 3 Data bits Selectable 7 or 8 data bits 4 Party bit Select...

Page 56: ...31 EN 6 2 2 End of the day wash screen When the program is shut down a pop up screen appears with End of day wash message To perform an End of day wash exchange the rinse bottle with the cleaning solu...

Page 57: ...continuous 2 On Flash Modulated 3 Off Motor mode Control to select one of the following motor modes 1 Run until in home position 2 Run continuous If in the home position the motor can be re activated...

Page 58: ...Lorrca MaxSis Program Page 56 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 6 3 2 Hardware check Camera screen...

Page 59: ...Lorrca MaxSis Program Lorrca Maxsis User Manual Page 57 Version 5 04 MRN 231 EN 6 3 2 1 Hardware check Camera Led on screen 6 3 3 Hardware pO2...

Page 60: ...n 5 04 MRN 231 EN 6 4 Settings screen Before a measurement can be started check if the chosen settings are the appropriate ones either the default settings or customized settings You can always revert...

Page 61: ...dvisable to backup and delete this file from the hard disk regularly before its size exceeds the applicable maximum ENUMERATE FILE NUMBER With this selection each measurement is stored in a separate f...

Page 62: ...a Shear stress applied during camera adjustment diaphragm 0 100 Pa Bob temperature C Desired bob and hence suspension temperature in degrees Centigrade 20 45 C Auto start if temp is stable within C If...

Page 63: ...surement shear stress This parameter defines the initial cell stabilisation time following camera adjustment If the standard deviation of the elongation measurement at the first measurement shear stre...

Page 64: ...a newly acquired diffraction image to find the average EI and SD per deformability measurement 1 200 6 4 4 Aggregation This section gives a short description for all Settings parameters that influenc...

Page 65: ...g AUTO In auto mode all models are evaluated and the program will present the best fitting model The result of other models can still be examined and printed Mathematical model used to fit the syllect...

Page 66: ...aken once per revolution at the home position The program records both the mean back scatter and its standard deviation 2 200 Maximum deviation au When a new shear rate is adjusted the aggregation sta...

Page 67: ...ts and if intermediate disaggregation is not enabled it is possible to study memory effects hysteresis of RBC aggregation vs shear rate Iteration shear rates Presets values to which the iteration proc...

Page 68: ...ition parameters of the iteration procedure Samples per shear rate Maximum deviation Iteration timeout also apply to the FSAR measurement Low shear rate 1 s Low shear rate for determining FSARIscglow...

Page 69: ...tion on off Logarithmic pO2 axis Checkbox pO2 scan shear stress PA 0 100 Determine pO2 every s 0 3600 Moving average size 0 40 Calc area between 0 250 and mmHg 0 250 Scan steps see pO2 Steps on page 6...

Page 70: ...Lorrca MaxSis Program Page 68 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 6 4 6 1 pO2 Steps If pO2 control is disabled...

Page 71: ...Lorrca MaxSis Program Lorrca Maxsis User Manual Page 69 Version 5 04 MRN 231 EN If pO2 control is enabled...

Page 72: ...Lorrca MaxSis Program Page 70 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN Select HARDWARE SETTINGS at the top of the SETTINGS screen Screen Instrument settings appears...

Page 73: ...on Range Setting BOB radius mm Outer radius of the static bob in millimeters 5 50 mm CUP radius mm Inner radius of the rotating cup in millimeters 5 50 mm Camera cam1 pO2 Sensor batch number used for...

Page 74: ...1 Serial output comport ASRL1 INSTR By default 2 Baud rate Selectable 1200 4800 9600 38400 and many more default is set on 115200 3 Data bits Selectable 7 or 8 data bits 4 Party bit Selectable none s...

Page 75: ...Lorrca MaxSis Program Lorrca Maxsis User Manual Page 73 Version 5 04 MRN 231 EN pO2 Connection 6 5 Osmoscan screen...

Page 76: ...ell shape 4 Measurement and sample details 5 Measurement results 6 Parameters 6 5 1 Osmoscan result screen At measurement completion measurement results are displayed on screen the software offers an...

Page 77: ...ear stress was performed Time s Time in seconds since the beginning of the measurement Coordinate count Number of coordinates in the iso intensity curve that describes the shape of the diffraction pat...

Page 78: ...r 2 5 ml 4 Press RINSE to remove sample and rinse the system Sample is lost and no longer available Screen EMPTY CUP appears Press CLEAN 5 Remove empty rinse tube 6 Press OK and the Osmoscan screen ap...

Page 79: ...tarts after clicking NEW F1 The figure shows the window that appears before starting a scan See Settings for details on the Settings parameters that influence the measurement A measurement starts afte...

Page 80: ...ting a deformability measurement See Deformability curve on page 60 for details on the Settings parameters that influence the measurement A measurement starts after subsequently clicking NEW F1 On thi...

Page 81: ...n option to print yes no It is possible to print a measurement report by clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results CSV If enable...

Page 82: ...of coordinates in the iso intensity curve that describes the shape of the diffraction pattern A pixels Vertical axis of ellipse B pixels Horizontal axis of ellipse EI Elongation index Eof pixels Erro...

Page 83: ...an now be started or return to the main menu by pressing MAIN MENU to perform other tests Or exit the software and perform an END OF DAY WASH on page 142 6 7 Oxygenscan screen This screen appears when...

Page 84: ...ffers an option to print yes no It is possible to print a measurement report by clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results CSV If...

Page 85: ...time End time Actual time that was valid after the last EI measurement at the given shear stress was performed Time s Time in seconds since the beginning of the measurement Coordinate count Number of...

Page 86: ...no longer available Screen EMPTY CUP appears Press CLEAN 5 Remove empty rinse tube 6 Press OK and the Oxygenscan screen appears 7 Press F1 or click on NEW F1 to perform another Oxygenscan or Press MAI...

Page 87: ...ting a stability test See Stress curve determination on page 61 for details on the Settings parameters that influence the measurement A measurement starts after subsequently clicking NEW F1 On this sc...

Page 88: ...offers an option to print yes no It is possible to print a measurement report by clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results CSV I...

Page 89: ...en shear stress was performed Time s Time in seconds since the beginning of the measurement Coordinate count Number of coordinates in the iso intensity curve that describes the shape of the diffractio...

Page 90: ...bility test on page 62 Settings for details on the Settings parameters that influence the measurement The cell membrane stability test consists of 3 stages A measurement starts after subsequently clic...

Page 91: ...nt yes no It is possible to print a measurement report by clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results CSV If enabled the results w...

Page 92: ...o remove the blood sample Press CLEAN Sample is removed and system is rinsed Sample is lost and no longer available A new measurement can now be started or return to the main menu by pressing MAIN MEN...

Page 93: ...re offers an option to print yes no It is possible to print a measurement report by clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results CS...

Page 94: ...rs an option to print yes no It is possible to print a measurement report by clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results CSV If en...

Page 95: ...es in the iso intensity curve that describes the shape of the diffraction pattern A pixels Vertical axis of ellipse B pixels Horizontal axis of ellipse EI Elongation index Eof pixels Error of fit This...

Page 96: ...at the given shear stress was performed Time s Time in seconds since the beginning of the measurement Coordinate count Number of coordinates in the iso intensity curve that describes the shape of the...

Page 97: ...or Crd cnt Number of coordinates in the iso intensity curve that describes the shape of the diffraction pattern A pixels Vertical axis of ellipse B pixels Horizontal axis of ellipse EI Elongation ind...

Page 98: ...ion on page 62 settings and the following option selections for details on the Settings parameters that influence the measurement 1 Disaggregation level 2 Syllectogram following cup cessation 3 Iterat...

Page 99: ...tware offers an option to print yes no It is possible to print a measurement report by clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results...

Page 100: ...oughout the syllectogram Peak intensity usually the top of the syllectogram Isc Back scatter intensity at t Isc Isc top Amp 2 Isc0 Syllectogram base level average of last 40 samples Note that the syll...

Page 101: ...eaux formation time constant Is RBC 3D aggregate formation contribution s slow Ts RBC 3D aggregate formation time constant I0 Syllectogram base level Iteration Isc max Highest back scatter intensity f...

Page 102: ...RBC Aggregation After a measurement has completed the program sets the fill empty speed SETTINGS FILL EMPTY SPEED and asks the operator to remove the blood sample Press RINSE Sample is removed and sy...

Page 103: ...MRN 231 EN 7 GENERAL OPERATION PROCEDURE 7 1 Powering up Switch ON the Lorrca Switch ON the PC and printer Start Lorrca software Steady state After switching on the Lorrca MaxSis needs 5 minutes to w...

Page 104: ...es 7 2 1 Lorrca reagents The Lorrca reagents are delivered in 5 ml vials Elon ISO and Oxy ISO or in storage bottles of 0 5 l Osmo HIGH Osmo LOW In all cases Store the reagent as indicated at the packa...

Page 105: ...Check for turbidity and discard if turbidity develops Do not return reagents from the instrument bottles back in storage bottles After opening the reagents can be used during one week stored at 4 7 C...

Page 106: ...000 RBC 7 2 4 Oxygenscan whole blood volume calculation Measure RBC level CBC on a Cell Counter 250 RBC xx L blood in 5 ml Oxy ISO RBC in 106 l or RBC in 1012 l this should result in a value between...

Page 107: ...ubing and connections have a free passage and are not folded or clogged 6 Remove priming tube from the needle position 7 Return to main screen In case of starting the day with Osmoscan Prepare a blood...

Page 108: ...General operation procedure Page 106 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 7 3 2 Place sample tube Osmoscan screen...

Page 109: ...is the viscosity parameter of the test sample which is mainly determined by the reagent The indicated value differs per batch and have to be entered at the start of each test run See chapter Lorrca r...

Page 110: ...ern image fills the entire viewing field green light upper right of screen In case the auto start is enabled the measurement starts automatically when the osmolality has reached a value less than 75 m...

Page 111: ...clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results CSV If enabled the results will also be written to other files see Settings Files on...

Page 112: ...RINSE to remove sample and rinse the system Sample is lost and no longer available Screen EMPTY CUP appears Press CLEAN 5 Remove empty rinse tube 6 Press OK and the Osmoscan screen appears 7 Press F1...

Page 113: ...7 4 1 Preparing a blood sample Use the dedicated Lorrca reagents see Appendix Parts list on page 191 A blood sample can be stored at room temperature during a limited time After that the sample canno...

Page 114: ...04 MRN 231 EN 2 Gentle mix the blood by immediately inverting the tube 5 10 times don t shake until it colours homogeneous red 3 Click on DEFORMABILITY CURVE on the Main screen The Stability start scr...

Page 115: ...easurement data if enabled in the Settings menu The REMARKS and DONATION TIME fields may be ignored but serve to include sample details The VISCOSITY field is the viscosity parameter of the test sampl...

Page 116: ...s Aspirate and check if the suspension level is high enough and without air bubbles Capillary forces usually keep the suspension within the gap However if capillary forces are insufficient more of tes...

Page 117: ...ect value Press START NOW The measurement starts and the curve starts to appear 7 4 4 Deformability curve result screen At measurement completion measurement results are displayed on screen the softwa...

Page 118: ...completed and the report is printed yes no Press the YES for printed report Press the NO if no report is required Screen EMPTY CUP appears 1 Press CLEAN 2 Open the hood 3 After a measurement on whole...

Page 119: ...xit the software and perform an END OF DAY WASH on page 142 7 5 Performing Cell stability test Start software 7 5 1 Preparing a blood sample Use the dedicated Lorrca reagents see Appendix Parts list o...

Page 120: ...4 MRN 231 EN 2 Gentle mix the blood by immediately inverting the tube 5 10 times don t shake until it colours homogeneous red 3 Click on CELL STABILITY TEST on the Main screen The Stability curve star...

Page 121: ...measurement data if enabled in the Settings menu The REMARKS and DONATION TIME fields may be ignored but serve to include sample details The VISCOSITY field is the viscosity parameter of the test sam...

Page 122: ...ess Aspirate and check if the suspension level is high enough and without air bubbles Capillary forces usually keep the suspension within the gap However if capillary forces are insufficient more of t...

Page 123: ...value Press START NOW The measurement starts and the curve starts to appear 7 5 4 Stability curve determination results screen At measurement completion measurement results are displayed on screen the...

Page 124: ...completed and the report is printed yes no Press the YES for printed report Press the NO if no report is required Screen EMPTY CUP appears 1 Press CLEAN 2 Open the hood 3 After a measurement on whole...

Page 125: ...the software and perform an END OF DAY WASH on page 142 7 6 Performing Cell membrane stability test Start software 7 6 1 Preparing a blood sample Use the dedicated Lorrca reagents see Appendix Parts l...

Page 126: ...1 EN 2 Gentle mix the blood by immediately inverting the tube 5 10 times don t shake until it colours homogeneous red 3 Click on CELL MEMBRANE STABILITY TEST on the Main screen The Cell membrane stabi...

Page 127: ...by the reagent The indicated value differs per batch and have to be entered at the start of each test run See chapter Lorrca reagents on page 102 for obtaining batch information Always check whether...

Page 128: ...raction pattern It is optional to adjust the camera gain bar on the right side until the diffraction pattern image fills the entire viewing field green light upper right of screen In case the auto sta...

Page 129: ...tware offers an option to print yes no It is possible to print a measurement report by clicking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results...

Page 130: ...cking the PRINT REPORT button The latest results automatically overwrite the results file Default name Results CSV If enabled the results will also be written to other files see Settings Files on page...

Page 131: ...hole blood use the automated rinse cycle and simultaneous rinse the funnel with a spray bottle with de ionized water to clean and empty the CUP 4 Press F1 or click on NEW F1 to perform another measure...

Page 132: ...General operation procedure Page 130 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 7 7 Performing RBC Aggregation test Start software...

Page 133: ...are dependent on the amount of oxygenated Hb present it is important to use fully oxygenated whole blood When the degree of oxygenation is different in two samples of the same blood all absolute Isc...

Page 134: ...or RBC test on page 131 first Lower the BOB if necessary Start a new measurement by clicking the NEW F1 button in the screen Enter measurement ID The measurement ID is not allowed to contain character...

Page 135: ...of the blood suspension in the gap between BOB and CUP using a pipette see above Figure Press Aspirate and check if the suspension level is high enough and without air bubbles Capillary forces usually...

Page 136: ...tion result screen At measurement completion measurement results are displayed on screen the software offers an option to print yes no It is possible to print a measurement report by clicking the PRIN...

Page 137: ...completed and the report is printed yes no Press the YES for printed report Press the NO if no report is required Screen EMPTY CUP appears 1 Press CLEAN 2 Open the hood 3 After a measurement on whole...

Page 138: ...Manual Version 5 04 MRN 231 EN 4 Press F1 or click on NEW F1 to perform another measurement or Press MAIN MENU to perform other tests perform in that case also an END OF DAY WASH on page 142 or Exit...

Page 139: ...at least 24 hours to accommodate to the level of dissolved oxygen corresponding to ambient temperature 7 8 1 Pre test preparations Start up 1 Switch pc and Lorrca on 2 Open the nitrogen cylinder 3 Lo...

Page 140: ...4 Gently pipet calculated standardized sample volume into the Oxy ISO 5 Gently mix the Oxy ISO vial 40x use the blood Oxy ISO solution within 30 minutes Oxy ISO is not a storage medium 6 Slowly draw 2...

Page 141: ...re Lorrca Maxsis User Manual Page 139 Version 5 04 MRN 231 EN 3 Initiate test by starting new measurement Click on NEW F1 4 Add sample data do not click any button and viscosity data only for the firs...

Page 142: ...d 7 Click Aspirate to accurately levelling the solution in the cup This will take 60 seconds If the edges are not sharp enough click Aspirate again 8 Verify that the edges of the aspiration patch in t...

Page 143: ...ing a large syringe If everything is dry lower the Bob in the cup Ready to run the next sample The Oxygen sensor spot is tested during the self check before every measurement In case the self check is...

Page 144: ...yringe filled with de ionized water 4 Press CLEAN and slowly flush the connector during rinsing 5 Dry the Bob Cup and the connector with cotton paper towel and air to assure the tube is free of water...

Page 145: ...aning solution bottle 3 Place a tube with cleaning solution at sample position 4 Follow the software instructions 5 Remove after finishing of the End of day wash the cleaning tube from the reagent tub...

Page 146: ...he instrument is running Always wait until the instrument has completed its cycle Go to HARDWARE CHECK LORRCA IO Press RINSE CUP Press RINSE GRADIENT PUMP If available Dry CUP and BOB Do not scratch t...

Page 147: ...d then carefully wipe up use protection gloves Pick up any glass using forceps Once all the material has been removed disinfect the area thoroughly Inform the Technician in Charge of the spill 7 12 Wa...

Page 148: ...Reporting Page 146 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 8 REPORTING Reporting examples 8 1 Deformability Report See Deformability curve determination parameters explanation on page 80...

Page 149: ...Reporting Lorrca Maxsis User Manual Page 147 Version 5 04 MRN 231 EN 8 2 Cell Membrane Stability report page 1 of 3 See Deformability results 1 parameters explanation on page 93...

Page 150: ...Reporting Page 148 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 8 3 Cell Membrane Stability report page 2 of 3 See Stability results parameters explanation on page 94...

Page 151: ...Reporting Lorrca Maxsis User Manual Page 149 Version 5 04 MRN 231 EN 8 4 Cell Membrane Stability report page 3 of 3 See Deformability results 2 parameters explanation on page 95...

Page 152: ...Reporting Page 150 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 8 5 Osmoscan Report See Osmoscan parameters explanation on page 75...

Page 153: ...ined personal They should be familiar with at least the operation and various basic settings Do not hesitate to contact RR Mechatronics if you are confronted by a fault which is not described in this...

Page 154: ...oftware Error 8 Home sensor is not sensed during initiation of the servomotor Exit software and switch off the LoRRca Check communication cables to computer Switch on the LoRRca and start the software...

Page 155: ...on Exit software and switch off the LoRRca Check communication cables to computer Switch on the LoRRca and start the software Check in Settings screen on page 58 that the Osmoscan option Checkbox is s...

Page 156: ...ice has to be sent to RRM for service Photo diode saturated Ambient light too high or signal strength too high Send measurement sensor data Phase Amplitude Temperature and Calibration data ideally the...

Page 157: ...ration file Wrong calibration file installed or file is corrupted Check calibration file Unknown calibration mode Unknown calibration mode in calibration file Wrong calibration file installed or file...

Page 158: ...tion screen Adjust projection screen if necessary see Adjusting projection screen on page 165 Fluid flow There is no fluid flow or not enough fluid flow in one or more tubes Check the liquid levels in...

Page 159: ...he schedule shows which maintenance procedures are allowed to be executed by an operator The maintenance recommendations are based on an 8 hour working day The frequency must be increased with shift w...

Page 160: ...Description Daily Weekly Monthly 6 monthly yearlly Clean X Reagent handling X Visual check X Pump tube replacement X Periodical maintenance X Report irregularities in the logbook The yearly maintenan...

Page 161: ...ble fluids is not allowed Do not use medical alcohol bleach or any solvent for cleaning use only DI water or Starrsed X clean Clean the casing with a soft dust free cloth moistened with a few drops of...

Page 162: ...time the pO2 measured by the spot will decline due to the influence of light and mechanical wear resulting in an inaccurate reading of the real ambient pO2 Correcting the decline in the pO2 measureme...

Page 163: ...ce the spot by a new one provided in a black plastic pouch by pressing it into the outside of the cup 3 A new sensor spot is accompanied by a data file containing the calibration data of the new spot...

Page 164: ...restarted it will notice that the original sensor spot datafile is missing The new file will need to be linked through the settings screen and then selecting the tab instrument settings and from ther...

Page 165: ...pure water Drain the bottles as much as possible and let it dry sufficiently Do not forget to change the default viscosity value in case of a new reagent batch at SETTINGS SCREEN GENERAL OPTIONS Batch...

Page 166: ...ed here or rename any available memory stick to LoRRca backup Place the memory stick in the USB slot of the computer or the USB extension cable Drag the file C LoRRca Maxsis Results onto the memory st...

Page 167: ...in Appendix Parts list on page 191 10 10 1 Adjusting projection screen Switch ON the instrument Start up the PC Choose LoRRca software program Select HARDWARE CHECK Move the Bob down Choose CAMERA Se...

Page 168: ...laser spot Note The projection screen holder itself can also be adjusted equipped with slotted holes Tighten the screws of the projection screen holder Not OK Driver failure Update the camera driver...

Page 169: ...Maintenance Lorrca Maxsis User Manual Page 167 Version 5 04 MRN 231 EN 10 10 2 Adjusting camera for slides...

Page 170: ...04 MRN 231 EN Place the EI 0 slide Adjust the camera gain according the value on the slide Close the hood Turn on the led Place the EI 0 333 slide Adjust the camera gain according the value on the sli...

Page 171: ...a Maxsis User Manual Page 169 Version 5 04 MRN 231 EN Adjust the camera gain according the value on the slide Close the hood Turn on the led The pattern must be uniform red The measured value should m...

Page 172: ...he power voltage is 115VAC another fuse has to be placed see ID plate Pull the fuse holder in the main power entrance module open with a screwdriver or other flat tool The main power entrance is locat...

Page 173: ...es QWLV090011 3 2mm tube and the rinse pump uses QWLV090012 1 6mm tube Always replace the correct tube The pumps are in the foot of the Lorrca MaxSis pull on the lid to open the pump drawer The pump t...

Page 174: ...h label 6 which is shown GREEN in the picture Next picture The installed tube is now BLUE continues with the next GREEN tube 1 Connect tube with label 6 to the output port of the Rinse pump 2 Connect...

Page 175: ...a Maxsis User Manual Page 173 Version 5 04 MRN 231 EN Tube to needle assembly LORC070901 Connect tube with label 5 onto connector 5 Connect tube with label 3 onto connector 3 Connect tube with label 2...

Page 176: ...Maintenance Page 174 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 10 10 5 Tubing replacements Tubing as used for Osmoscan...

Page 177: ...5 1 Gradient pump tube replacement After 500 measurements half year Disconnect water and reagent tubing and pull up the sample needle Lower the BOB and perform two times a PRIME ALL function HARDWARE...

Page 178: ...with new tube assembly following Install LORC070901 tubing on page 177 see also Appendix Parts list on page 191 spare and replacement parts 10 10 5 2 Install LORC070901 tubing Put the waste tubing la...

Page 179: ...5 04 MRN 231 EN Continue the with the four other tube brackets Hook the first tube bracket onto the pump frame Waste bracket Continue the with the rest of the tube brackets This how it looks when all...

Page 180: ...ca Maxsis User Manual Version 5 04 MRN 231 EN Connect tube with label 5 onto connector 5 Connect tube with label 3 onto connector 3 Connect small sample tube onto the first cassette Connect tube with...

Page 181: ...both small tubes into the pinch valve V4 short end at the front 2 Connect tube with label 2 on the connector 2 3 Put both small tubes into the pinch valve V3 The tube with label Osmo HIGH is at the ba...

Page 182: ...age 180 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 7 Connect the tube 1 onto connector 1 Tube to needle assembly 1 Connect tube with label 3 on the connector 3 2 Connect the other end to the ne...

Page 183: ...th the next GREEN tube Start with tube end with label 5 1 Put the short tube into the pinch valve V2 in the back port 2 Connect tube with 5 on the connector 5 3 Put the other short tube into the pinch...

Page 184: ...RN 231 EN 8 Take the long end of the tube and run this through the front hole 9 Connect tube 4 on the bob connect tube 6 on the EC sensor Tubing to waste connector Connect tube with label 7 on the con...

Page 185: ...th the next GREEN tube 1 Connect tube with label 6 to the output port of the Rinse pump 2 Connect tube with label 1 to the Input port of the Rinse pump 3 Connect tube with label 7 to the output port o...

Page 186: ...End of day wash to clean all tubing 2 Disconnect tubing from bottles except the waste line 3 Perform a prime all and a cup rinse step to empty all tubing 4 Clean the outside of the instrument carefull...

Page 187: ...Appendix for Lorrca MaxSis Lorrca Maxsis User Manual Page 185 Version 5 04 MRN 231 EN 11 APPENDIX FOR LORRCA MAXSIS Appendix section...

Page 188: ...ry storage In case of long time inactivity or temporary storage 1 Perform an End of day wash to clean all tubing User Manual General operation procedure End of Day Wash 2 Disconnect tubing from bottle...

Page 189: ...Lorrca Maxsis User Manual Page 187 Version 5 04 MRN 231 EN 11 2 Instructions for packing 11 3 Part 1 applicable for all types Use the packing foam to secure the cup as shown in the picture...

Page 190: ...231 EN This picture is from a Lorrca without a cup Please do not remove the cup 11 4 Part 2a applicable for Lorrca 109001 Use another piece of foam and cut two blocks in the following size with a cut...

Page 191: ...1 on the left and block 2 on the right over the side of the pump plate to secure it 11 5 Part 2b applicable for Lorrca109003 Use safety plate yellow green in the picture to secure the pinch valves Ti...

Page 192: ...ed by the quantity required For example PART NUMBER QUANTITY QWLV090011 1x Make a clear distinction between the capital letters O and D and the number 0 When reading certain numbers on the drawings mi...

Page 193: ...ube bore 3 2 mm 1 QWLV090012 Peristaltic Pump tube bore 1 6 mm 1 QEDV130017 Fuse 1 6A Slow 230VAC 2 QEDV130020 Fuse 3 15A Slow 115VAC 2 Replacement parts Part No Description LORC110900 Rinse bottle LO...

Page 194: ...030901 Elon ISO 100 tubes of 5 ml in tray QRR 030905 Oxy ISO 100 tubes of 5 ml in tray QRR 030910 Osmo LOW 0 5 Ltr QRR 030930 Osmo HIGH 0 5 Ltr QRR 010946 Starrsed X Clean 5 Ltr Accessories Part No D...

Page 195: ...ient Peristaltic pump LORC070902 Tubing set 1 LORC070903 Tubing set 2 LORC070904 Tubing set 3 LORC070905 Cleaning solution tube LORC070906 Tubing set 4 QEDV130018 Fuse 2 A Slow QEDV130010 Fuse 315 mA...

Page 196: ...nstallation Operational instructions Maintenance Mains specification changed Oxygenscan procedure changed Cleaning instructions changed Document History added as appendix only latest changes in introd...

Page 197: ...e is changed from 3500 to 800 PVP handling items Calibration is not applicable for the LoRRca and all issues are removed out of the manual H Schavemaker 4 01 January 2012 Yearly update and publishing...

Page 198: ...ix for Lorrca MaxSis Page 196 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN Appendix Additional Information This is additional information about the basic principle of the performed test with the...

Page 199: ...t 15 16 The instrument was made commercially available by R R Mechatronics38 up to 2008 under the name LoRRca In 2009 the original hardware was improved and extended with the facility to measure red c...

Page 200: ...A is therefore an indispensable instrument for hemorheological laboratories in research institutes clinical departments of hematology intensive care units etc It uses a Couette geometry with a static...

Page 201: ...hear rate 1 s as given above equation Equation 2 11 6 2 1 RBC deformability measurements The red blood cells in EDTA anticoagulated blood diluted 1 200 in a viscous reagent detailed in section 2 1 2 a...

Page 202: ...rom circular at rest to elliptical at a high shear stress The major a and minor b axis of the ellipse serve to calculate the elongation index EI a b a b The photo diodes are used for aggregation measu...

Page 203: ...y deformed by a user defined high shear stress for a certain time period The effect on the cells can be followed either during the stress application period by the intermittent measurements of their d...

Page 204: ...ar effect was observed using the LORCA in mixtures of rigid and normal deforming RBC both in the experimental REF and clinical situation e g in the blood from patients with sickle cell disease REF How...

Page 205: ...rejects patterns if the remaining number of pixels drops below a specified number 50 Distance to ellipse 8 1 Ahn S J Rauh W Geometric least squares fitting of circle and ellipse Int J Pattern Recognit...

Page 206: ...five ellipse parameters indicated location x0 y0 orientation and axes a b B the closest distance of a data point to the best fit ellipse is estimated using the confocal hyperbola that goes through the...

Page 207: ...increased by fitting more than one intensity level at the cost of computational speed 11 6 2 2 RBC aggregation and relaxation measurements The state of RBC dis aggregation is shear rate dependent at...

Page 208: ...ceeds A mathematical representation of the syllectogram yields aggregation parameters that are used to assess RBC aggregability References on page 215 6 23 31 Figure 5 LORCA aggregometer setup Figure...

Page 209: ...ting larger 3D aggregates In normal human blood the formation of 3D aggregates is a slower process Reference 622 with a time constant of about 10 25 s 21 Groner W Mohandas N Bessis M New optical techn...

Page 210: ...y parameters from conventional parameters not resulting from the curve fit the latter gives the symbol Isc 23 Bauersachs R M Wenby R B Meiselman H J Determination of specific red blood cell aggregatio...

Page 211: ...er to display the results of a fixed syllectogram model or to display the best performing one auto mode Mode Equation Relevant program parameters Tri exp rise fall fall Ir Tr RBC relaxation If Tf RBC...

Page 212: ...er solution Uni exp fall If Tf RBC rouleaux formation Describes curve as if only one exponent would describe RBC aggregation 36 Bauersachs R M Wenby R B Meiselman H J Determination of specific red blo...

Page 213: ...eshold shear rate When intermediate disaggregation is enabled which is preferable the difference between the back scatter during disaggregation and that at each subsequent user defined shear rate dIsc...

Page 214: ...m the peak value in the Syllectogram Figure 8 Iteration procedure without intermediate disaggregation Figure 8 Iteration procedure without intermediate disaggregation After disaggregating the sample o...

Page 215: ...r peaks at both sides of the zero order peak represent the higher orders When opening the diaphragm light intensity increases hereby increasing also the higher order patterns As soon as the intensity...

Page 216: ...User Manual Version 5 04 MRN 231 EN 0 Black 1 Fit gray level Dark Light blue Fit gray level Fit gray level higher levels Black Rest 254 Dark Light red 255 White Table Colour look up table definition f...

Page 217: ...normal shape altered and pathological red cells Blood cells vol 1 pp 315 321 1971 9 Bessis M Mohandas N Feo C Automated ektacytometry A new method of measuring red cell deformability and red cell ind...

Page 218: ...r and extracellular markers with film in Red Cell Rheology Springer Verlag Berlin pp 347 361 1978 ISBN 3 540 09001 0 25 Groner W Mohandas N Bessis M New optical technique for measuring erythrocyte def...

Page 219: ...is M Red blood cell deformability and hemolytic anemias Semin Hematol vol 16 2 pp 95 114 1979 43 Mokken F Ch Kedaria M Henny Ch P Hardeman M R Gelb A W The clinical importance of erythrocyte deformabi...

Page 220: ...treekstra G J Hoekstra A G Nijhof E J Heethaar R M Light scattering by red blood cells in ektacytometry Fraunhofer versus anomalous diffraction Appl Opt vol 32 pp 2266 2272 1995 59 Sutera S P Mueller...

Page 221: ...wings and parts Lorrca MaxSis Lorrca Maxsis User Manual Page 219 Version 5 04 MRN 231 EN 12 DRAWINGS AND PARTS LORRCA MAXSIS Drawing section 12 1 LORC070020 Tubing diagram Parts LORC070020 Tubing diag...

Page 222: ...Drawings and parts Lorrca MaxSis Page 220 Lorrca Maxsis User Manual Version 5 04 MRN 231 EN 12 2 LORC080020 Connection diagram...

Page 223: ...5 04 MRN 231 EN 13 LOG BOOK EXAMPLE Name Date Description visual inspection OK NOT OK REMARKS Scratches on glass surface of cup Check tube attachments Check tube damage Description functional test OK...

Page 224: ......

Page 225: ...Center in Amsterdam Netherlands E 14 1 1 1 2 EI EI expressed as elongation index H 14 1 1 1 3 HE HE stands for Hereditary Elliptocytosis L 14 1 1 1 4 Lorca Lorca stands for Laser assisted Optical Rota...

Page 226: ......

Page 227: ...ne stability results screen 91 127 Cell Membrane stability screen 51 88 90 93 94 Checking Lorrca MaxSis functionality 47 Cleaning 159 Conditions 40 D Decontamination procedures Routine 145 Deformabili...

Page 228: ...BC aggregation 26 Introduction RBC elongation 26 Iteration parameters setting 64 Iteration procedure 211 L Laser and mechanical safety 31 LOG BOOK EXAMPLE 221 LORC070020 Tubing diagram 29 43 219 LORC0...

Page 229: ...O P for working with bio hazardous materials 35 Safety 12 SAFETY 28 Safety for working with compressed Nitrogen 33 Screen communication settings 53 71 Settings screen 58 153 Specific laboratory pract...

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