Last Updated on Sep 2021
4.1 Switching ON Protocol
(a)
SIGN IN the Logbook and record your START-TIMING.
(b)
Switch on Laser Key
(1)
, Laser Power
(2)
, Scanner power
(3)
and PC Microscope
(4)
.
(c)
Switch on the Metal Halide lamp
(5)
, if you need it.
(d)
Switch on STED laser module
(6)
, if you need it.
(e)
Wait for PC to start up and log in with your TLL user name.
(f)
Load LASx software and switch on the lasers that you are planning to use:
•
Diode Laser
: “ON” 405nm laser
•
White Light laser
: “ON” up to 70% power
•
Argon laser
: “ON” up to 70% power.
(Make sure the argon lasers are ON for a
minimum of 2hrs before switching OFF as it requires sufficient time for warming up)
•
STED laser
: You may switch
“ON” up to 100%.
(Make sure the STED lasers are ON for
at least 1hr before switching OFF.)
(g)
Do not overload the HyD detectors. Keep gain at max 100% as overloading will damage
the performance of HyD detectors over time. For best performance of the HyD detectors,
set gain at 100% and only use the laser power slider to moderate irradiation intensity.
(h)
Finally, fill in the logbook.
4.2 Objective Lens
Objective lens
Immersion media
10x/0.4 HC Plan Apochromat
Air
20x/0.75 HC Plan Apochromat
Air
40x/1.3 HCX Plan Apochromat
Air
63x/1.2 HC Plan Apochromat
Water
63x/1.4 HC Plan Apochromat
Oil
100x/1.4 HCX Plan Apochromat
Oil
