Last Updated on Sep 2021
•
If you want to also acquire a brightfield image activate the
Transmission channel.
o
Ensure that there is at least one active laser emission line
when you are using this channel
4.7 Using the Acquisition tab
Select the
Acquisition
tab, in which you can set the parameters for your imaging:
•
Acquisition Mode:
➢
Choose the type of scan xyz (z-stack) or xyzt
(time-series) are the most commonly used
➢
Seq
: select this for multi-channel/colour samples
•
XY
➢
Format
: the number of pixels in your image
(the more pixels the higher the resolution,
to an extent, but slower the acquisition). The max
is
8000x8000.
➢
Speed
: the slower, the better your image will be
(a higher signal:noise ratio; but it takes longer and
it bleaches your sample more).
➢
Bidirectional X
: Useful for live imaging
unless you require fast imaging (in which case you
will need to adjust the phase correction.)
➢
Zoom:
is where you control the zoom parameters,
the arrows allow you to nudge the zoomed area
within the image. Zooming in can increase your
resolution to a point, but can cause more
photobleaching/damage.
➢
Average
: averages a given number of scans to get
a higher signal to noise ratio. Drawback: slower
scanning time and more bleaching. No averaging
or Line averaging is recommended if you are
doing live-cell imaging. Averaging twice will help
you achieve better quality images.
➢
Accumulation
: Is the sum of signal of several images together, hence boosting your
signal. Drawback: you also sum the noise and the resulting image may not have
improved signal:noise ratio, also has a slower scan time and more bleaching.
➢
Auto Gain: the program will adjust the gain (beware that this happens only for the
active channel if you are in
seq
mode).
