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Leica Emspira 3, User Manual

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Emspira 3 User Manual 

General Notes 7

Symbols Used

Warning! Safety hazard!

This symbol indicates especially 
important information that is 
mandatory to read and observe. 
Failure to comply can cause the 
following: 

 

O

Personal injury!

 

O

Emspira 3 malfunctions and damage.

Warning of hazardous electrical voltage

This symbol indicates especially 
important information. Failure to 
comply can cause the following: 

 

O

Personal injury!

 

O

Emspira 3 malfunctions and damage.

Microscope malfunctions and damage

This symbol warns against touching 
hot surfaces, such as light bulbs.

Important information

This symbol indicates additional 
information or explanations that are 
intended to provide clarity.

Read and understand this user 
manual before using this device.

Summary of Contents for Emspira 3

Page 1: ...Emspira 3 User Manual...

Page 2: ...umination LED3000 RL 28 Illumination LED3000 NVI 30 Illumination LED5000 SLI LED3000 SLI LED3000 DI 31 Installation Stand Alone Mode Cables and Terminals 34 Stand Alone Mode Installing Optional Extern...

Page 3: ...hnical Data 59 Optical Data Field of View 64 Optical Data Magnification and Depth of Field 66 Dimensional Drawings 68 Emspira 3 68 Emspira 3 With Incident Light Base 69 Emspira 3 With Incident Light B...

Page 4: ...Emspira 3 User Manual General Notes 4 General Notes...

Page 5: ...echniques for cleaning O Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abra ded particles Ser...

Page 6: ...booklet contains additi onal safety information regarding the service work requirements and the handling of Emspira 3 accessories and electrical accesso ries as well as general safety instructions You...

Page 7: ...of hazardous electrical voltage This symbol indicates especially important information Failure to comply can cause the following O Personal injury O Emspira 3 malfunctions and damage Microscope malfu...

Page 8: ...thorized alterations to Emspira 3 or noncompliant use shall void all rights to any warranty claims Place of use O Refer to Safety Concept booklet O Electrical components must be placed at least 10 cm...

Page 9: ...osal O Refer to Safety Concept booklet Legal regulations O Refer to Safety Concept booklet EC Declaration of Conformity O Refer to Safety Concept booklet EMC precautions Prior to the installation of E...

Page 10: ...e safety and EMC Our microscope has been designed produced and tested in compliance with O EN 61010 1 Safety requirements for elec trical equipment for measurement control and laboratory use Part 1 Ge...

Page 11: ...an encryp tion which follows the Advanced Encryption Standard AES with a block and key size of 128 bits During any firmware update this password file will be deleted and the User will need to reenter...

Page 12: ...Emspira 3 User Manual Introduction 12 Introduction...

Page 13: ...0 2 160 4K UHD To be used on 4K monitors O 1 920 1 080 Full HD This resolution provides the characteristic imaging perfor mance for a Full HD capable monitor Standard delivery 1 2 The Emspira 3 system...

Page 14: ...n on additional accessories such as objectives and adapters illumination or the dust cover for Emspira 3 please contact your Leica Microsystems authorized dealer You can find detailed descriptions of...

Page 15: ...SB Type A 3 ON OFF switch 4 PC power supply connection USB Type C 5 Connection for handswitch footswitch 2 5 mm phone jack 6 Monitor port stand alone mode 7 USB SPEED port for stand alone operation in...

Page 16: ...6 System Overview 1 2 4 3 5 6 This overview shows Emspira 3 in an example application with additional components 1 Emspira 3 2 Microscope carrier 3 Illumination 4 Focusing column 5 Baseplate 6 HD moni...

Page 17: ...Emspira 3 User Manual Assembly 17 Assembly...

Page 18: ...er shows an example of assembly of Emspira 3 on an incident light base Images and descriptions may vary when using another incident light base Assembling the column 2 2 1 Take the three provided screw...

Page 19: ...with a short working distance 2 2 Alternatively install the microscope carrier upside down recommended variant for objectives with a large working distance Mounting Emspira 3 2 1 3 2 1 Unscrew the cl...

Page 20: ...ry when using another transmitted light base Installing the column adapter 2 2 1 Take the three provided screws out of the packaging 2 Install the column adapter on the column using the three screws A...

Page 21: ...ided screw recommended variant for objectives with a small working distance 2 2 Alternatively install the microscope carrier upside down recommended variant for objectives with a large working distanc...

Page 22: ...this part of the setup General notes This chapter shows an example of assembly of Emspira 3 on a swing arm stand Images and descriptions may vary when using another swing arm stand Assembling the colu...

Page 23: ...n the safety ring You can now rotate the horizontal arm in any direction desired The swing arm stand is designed so that Emspira 3 can be moved as desired However it may also be logical in some instan...

Page 24: ...washer and safety screw Mounting Emspira 3 1 3 2 2 1 Unscrew the clamping screw 2 Place Emspira 3 in the microscope carrier so that the notch in the microscope housing and the lower clamping screw ov...

Page 25: ...Emspira 3 User Manual Objective and Illumination 25 Objective and Illumination...

Page 26: ...sassembly so that it does not fall onto the stage plate Remove all specimens from the stage plate first The objective is not part of the standard delivery of Emspira 3 2 2 Screw the compatible adapter...

Page 27: ...ctive firmly during assembly and disassembly so that it does not fall onto the stage plate Remove all specimens from the stage plate first The objective is not part of the standard delivery of Emspira...

Page 28: ...nect the external power supply unit 10 450 266 to the LED3000 RL 2 Attach the LED3000 RL to the objective from below 3 Tighten the locking screw The supported working distance is between 60 mm and 150...

Page 29: ...minator We recommend assembling the accessories on the LED3000 RL on a table in reverse and then mounting them on the objective together 1 1 Attach the optional polarization set polari zer and analyze...

Page 30: ...nect the external power supply unit 10450266 to the LED3000 NVI 2 Attach the LED3000 NVI to the objective from below 3 Tighten the locking screw The supported working distance is between 60 mm and 150...

Page 31: ...should be avoided for installation on a large incident light base 10450049 or a transmitted light base Leica Microsystems recommends the LED3000 SLI for installation on a small or medium incident ligh...

Page 32: ...power supply unit 10450266 to the LED5000 SLI LED3000 SLI or LED3000 DI The right and left side of the spot illumina tor both have a socket Second CTL2 socket An additional LED illuminator from Leica...

Page 33: ...Emspira 3 User Manual Installation 33 Installation...

Page 34: ...kit Using another adapter with incorrect voltage can substantially damage Emspira 3 The color of the LED indicates that the power supply is not sufficient it s red when the supply doesn t provide enou...

Page 35: ...ut formatting and handling the USB thumb drive provided in order to prevent malfunctions during image acquisition File system and formatting The exFAT or FAT32 file system is required for the thumb dr...

Page 36: ...ns Installation of the 10 monitor arm to the standard column is analogous to installation of the LED3000 SLI see Illumination LED5000 SLI LED3000 SLI LED3000 DI page 31 Proceed as follows to connect t...

Page 37: ...lation Guide 1 After downloading the software follow the instructions for installing the software The computer must have sufficient free hard drive space ON OFF SS HDMI SPEED 2 Connect Emspira 3 to a...

Page 38: ...Emspira 3 User Manual Installation 38 For information on adapting network and WLAN settings refer to the On Screen Display User Manual Network WLAN Settings...

Page 39: ...Emspira 3 User Manual Startup Procedure 39 Startup Procedure...

Page 40: ...ower supply detected O Only the preferred USB 2 0 port is powered O Further power reduction might be activated Stand alone mode Blue blinking O Emspira 3 is in fall back mode e g after a failed firmwa...

Page 41: ...K monitor and a power supply 1 Set the ON OFF switch to ON to switch on Emspira 3 During the boot sequence the status LED is blinking green and switches to a conti nuous white the moment it is ready t...

Page 42: ...d the live image is displayed on the computer Emspira 3 is ready to use 2 Start Leica Application Software X LAS X 3 Follow the instructions in the software for adjusting and acquiring an image For ad...

Page 43: ...essing the buttons hold the keypad between your thumb and index finger Avoid tapping the keypad with just one finger if possible Use 1 1 Switch on the illuminator by briefly pres sing the ON OFF butto...

Page 44: ...ght handed Adjusting the resistance Is the focusing drive too loose or too tight Does the equipment tend to slide downwards The resistance can be adjusted individually depen ding on the equipment weig...

Page 45: ...optionally be operated either with or without click stops enabled Continuous zoom is possible when the click stops are disabled On the other hand when the click stops are enabled photographs measure...

Page 46: ...Emspira 3 User Manual Operation Stand Alone Mode 46 Operation Stand Alone Mode...

Page 47: ...mation on setting up and switching on Emspira 3 in stand alone mode refer to chapters O Stand Alone Mode Cables and Terminals page 34 O Switching on Emspira 3 page 41 subchapter Stand alone mode page...

Page 48: ...Emspira 3 User Manual Operation PC Mode 48 Operation PC Mode...

Page 49: ...tor General notes When connected to a PC the microscope image is always shown in the preview window of Leica Application Software X In addition a HD 4K monitor can be connected via the HDMI interface...

Page 50: ...Emspira 3 User Manual Troubleshooting 50 Troubleshooting...

Page 51: ...tomatically to the necessary resolu tion However if no image appears and the screen reports an error message like out of range or something similar you can reset the resolution to 1 080 p How to reset...

Page 52: ...Emspira 3 User Manual Care and Maintenance 52 Care and Maintenance...

Page 53: ...rt flush the surface using a syringe or by brushing it off with a camel hair brush before attempting to wipe it clean O Optical surfaces should be cleaned using a lint free cloth lens cloth or cotton...

Page 54: ...ial alcohol When doing so follow the corresponding safety regulations O Remove dust with a bellows and a soft paintbrush O Clean the objectives with special optics cleaning cloths and pure alcohol Ser...

Page 55: ...26529 HDMI cable 2 m 2 19002015 C Mount Cap 3 10 726 837 USB C USB A cable 4 10726490 Power supply with USB C connector 5 10726491 Wireless USB mouse 6 10747551 USB stick 64 GB Optional Sales Item Ite...

Page 56: ...Emspira 3 User Manual Disposal 56 Disposal...

Page 57: ...Service or Leica Sales on how to dispose of it Like all electronic devices Emspira 3 its compo nents and accessories may not be disposed of as general household waste Please observe the national laws...

Page 58: ...Emspira 3 User Manual Specifications 58 Specifications...

Page 59: ...Apo 1 0x objective adap ter 10447176 and 10450651 and a stage micrometer 10310345 across the entire FOV using the On Screen Display and a 28 4k monitor Accu racy data were determined either using fact...

Page 60: ...measurement task 10450650 187 mm Achromatic 0 63 148 mm PlanApo 0 8x Adapter 10450652 Adapter for measurement task 10450651 112 mm PlanApo 1 0x Adapter 10450652 Adapter for measurement task 10450651...

Page 61: ...2 1011 mm2 Discharge time 2 seconds from 1 000 V to 100 V Click stop feature Eight switchable positions for repetitive tasks Standard objective Z Series planapochromat 1 0x Additional objectives Achr...

Page 62: ...on 15 W Supported software LAS X File formats JPG TIF BMP Color filter IR cut coating 650 nm not replace able Housing Characteristics Value Material PC ABS Antimicrobial surface AgTreatTM according to...

Page 63: ...m computer PC mode LAS X mode or by external power supply stand alone mode Min computer configuration Please consult the software used Tested standards EN 55011 EN 61326 1 EN 61010 1 Power supply opti...

Page 64: ...19 3 10 9 15 1 8 5 1 0 57 1 32 1 36 2 20 3 28 7 16 1 22 5 12 7 18 1 10 2 14 5 8 2 11 3 6 4 1 25 45 7 25 7 28 9 16 3 23 0 12 9 18 0 10 1 14 5 8 1 11 6 6 5 9 1 5 1 1 6 35 7 20 1 22 6 12 7 17 9 10 1 14 1...

Page 65: ...Objective PlanApo 2 0x PlanApo 5 0x Working distance 39 mm 19 mm Field of view zoom setting FOVx FOVy FOVx FOVy 0 75 12 0 6 8 4 8 2 7 1 0 9 0 5 1 3 6 2 0 1 25 7 2 4 1 2 9 1 6 1 6 5 7 3 2 2 3 1 3 2 0 4...

Page 66: ...14 61 1 0 09 4 0 15 1 1 4 24 1 0 6 30 1 0 36 39 1 0 22 48 1 0 14 60 1 0 09 77 1 0 06 5 0 19 1 0 9 30 1 0 4 38 1 0 23 48 1 0 14 60 1 0 09 75 1 0 06 961 1 0 04 6 0 23 1 0 6 36 1 0 3 45 1 0 16 58 1 0 10...

Page 67: ...2 96 1 0 036 2 0 48 1 0 14 120 1 0 023 2 5 60 1 0 09 150 1 0 015 3 2 77 1 0 06 192 1 0 006 4 0 96 1 0 036 240 1 0 009 5 0 120 1 0 0223 300 1 0 004 6 0 144 1 0 016 360 1 0 003 28 0 75 52 1 1 0 128 1 0...

Page 68: ...Emspira 3 User Manual Specifications 68 Dimensional Drawings Emspira 3 156 132 132 54 251 111 M60x1 29 3 29 3...

Page 69: ...Emspira 3 User Manual Specifications 69 Emspira 3 With Incident Light Base 278 301 278 119 116 301 244 324 24 max 457 66 255 156 181 468...

Page 70: ...Emspira 3 User Manual Specifications 70 Emspira 3 With Incident Light Base 420 325 126 154 24 max 658 534 510 262 325 420 156 181...

Page 71: ...Emspira 3 User Manual Specifications 71 Emspira 3 With Transmitted Light Base 154 154 45 69 346 69 346 412 156 181 max 523 378 223 3 115 384 284...

Page 72: ...Emspira 3 User Manual Specifications 72 Emspira 3 With Swing Arm Stand max 668 330 173 230 598 max 786 470 220 156...

Page 73: ...to modifications LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH CONNECT WITH US www leica microsystems com Leica Microsystems Schweiz AG Max Schmidheiny Strasse 201 9...

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