63
Objectives with engraving “0” (i.e. without
coverglass) and “–” are suitable for uncovered
specimens. If the microscope is used for both
covered and uncovered specimens, objectives
with the engraving “–” can be combined with
“0” as well as “0.17” objectives, without the
focal plane having to be reprogrammed, with the
exception of immersion objectives.
To store the focus data, always use a high-
contrast specimen where the same area is
suitable for all objective magnifications. For
transmitted light the specimen used for storing
the focus data should be as thin as possible in
order to have a defined focal plane even at
highest magnifications, e.g. a Leica stage
micrometer.
1.10 Storing the objective focus offsets
Focus the specimen with the objective with the
highest resolution (i.e. max. aperture/magnifica-
tion).
Attention:
When using immersion objectives (OIL, W, IMM):
release the locking mechanism of the front part
of the objective (page 51) to give the objective
the standard parfocalizing distance of 45 mm!
Accuracy can be enhanced by setting
variotubes and switchable tube lenses to a
higher magnification factor or by putting the
auxiliary telescope (Fig. 51) on the eyepiece.
Then set the upper threshold at this position
with the key (44.5) (display 0
µ
m!) and switch off
the microscope (42.14).
Pressing key (44.5) at the same time, switch the
microscope on again. “OK!” appears in the
display as long as the key (44.5) is pressed. After
the key has been released “Cal!” appears in the
display to indicate the storage of the focal plane
of the first objective. “0” is now stored as offset
for the focused objective. Now all the objectives
on the nosepiece can be focused.
!
Fig. 44
Motorfocus controls
Controls 1 – 4 are situated on both sides of the stand in the
same layout.
1
“Stepwidth”,
2
“Up”,
3
“Down”,
4
Focusing wheel,
5
“Upper threshold”,
6
“Lower threshold”,
7
Display
1
2
3
4
5
6
7
