Leica CM1860, Instructions For Use Manual, Page: 42 / 108

42
Version 2.0, Revision N
Daily Use of the Instrument
7
7. Daily Use of the Instrument
7.1 The sectioning process
Preparation
1. Install all necessary parts, whether standard or optional (e.g. panels, waste and brush tray and
the selected knife or blade holder). Make sure that they are mounted and cooled.
2. Set the chamber temperature according to the tissue type to be sectioned ( → P. 61 – 7.5
Temperature selection chart (in minus °C)) .
3. Freeze specimen onto a specimen disc (
→
P. 44 – 7.3 Specimen discs) and mount it into the
specimen head (
→
P. 44 – 7.3.1 Inserting the specimen discs into the specimen head) .
4. Make sure that the knife or blade is inserted in the knife or blade holder, see (
→
P. 47 – 7.4.4
Blade holder CE) or (
→
P. 54 – 7.4.6 Knife holder CN) .
5. Orient the specimen (
→
P. 44 – 7.3.2 Specimen orientation) .
6. If necessary, adjust the clearance angle ( → P. 46 – 7.4.2 Clearance angle adjustment) .
7. Check whether retraction is as wanted on or off (
→
P. 40 – 6.3 Retraction) .
Sectioning
1. Select trimming thickness.
2. Adjust anti-roll device ( → P. 51 – Adjusting the anti-roll guide system) .
3. Trim using the handwheel ( → P. 60 – Trimming the specimen) .
4. Select section thickness (
→
P. 39 – 6.2.7 Setting the section thickness) .
5. Carry out sectioning using the hand wheel, discard the first 2-3 sections.
6. Pick up the sections either by transferring it onto a cold slide using a cold brush or a warm slide.
Ending the sectioning process
1. Remove knife/blade out of the knife/blade holder.
Warning
The safety gloves included in the ( → P. 26 – 4.3 Standard delivery - packing list) must be worn when
inserting/removing the knife!
2. Remove the specimen from the cryostat e.g. fix it for later paraffin embedding.
3. Remove section waste using a cold brush.
4. Start UVC disinfection (
→
P. 33 – UVC disinfection (CM1860 UV only)) .
7.2 Specimen freezing
• Select the sectioning temperature (cryochamber temperature) according to the tissue type to be
sectioned ( → P. 61 – 7.5 Temperature selection chart (in minus °C)) .