70
Version 6.9, Revision H
Operating the Leica CM3600 XP
7
7.7 Setting the trimming parameters
Warning
• For reasons of safety and accuracy, the maximum trimming thickness should not exceed 100 μm
per section.
•
When trimming biological material (particularly bone), the section thickness should not exceed
50 μm.
Proceed as follows:
•
Open the
Main
window in the software.
•
Afterwards, adjust the desired section thickness, sledge speed, cutting window and section program,
see
.
•
Adjust the
Automatic
operating mode and then press the
Start
button, see
.
•
The trimming procedure will be carried out automatically.
7.8 Setting the sectioning parameters
Proceed as follows:
•
Open the
Main
window in the software and then set the
Manual
operating mode.
•
Afterwards, adjust the section thickness and the sectioning window, see
.
7.8.1 Sectioning the specimen
For the sectioning procedure, you need brush, pressure plate and special section collecting tape and –
depending on the application – a dehydration frame.
Note
The required accessories should be stored in the cryochamber for some time prior to sectioning to
make sure they have the same temperature as the specimen.
Proceed as follows:
•
Activate the knee lever, see
.
7.8.2 Collecting the sections
Prior to removing a section, an appropriately sized piece of tape must be attached to the specimen block
surface. When doing so, please comply with the following safety instructions: