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| LABNET 311DS SHAKING INCUBATOR
3.0 Operation
After installing the unit, you will need to power it up using the power switch in the back, then choose your sealing settings.
NOTE:
The Labnet AccuSeal™ plate sealer should be used only with microplates and seals that are designed for heat sealing. It is the
user’s responsibility to confirm that the consumables being used are appropriate for the application.
Proper heat sealing of microplates requires the proper temperature and time settings. Contact your consumable manufacturer for
recommended time and temperature parameters.
It is commonly required to try different temperature and sealing time settings to optimize the sealing of different types of plates.
Many polypropylene plates and sealing films seal well using a sealing temperature of 165°C and sealing time of 4.5 seconds.
3.1 Set the Temperature
Press the Mode button until the Seal Temp text is flashing. Use the control knob to adjust the display to the desired temperature.
Press the control knob or wait 10 seconds to set the selected temperature.
When a temperature is set above the current temperature of the heating platen, the display will show Heating and the Status
indicator LED above the door will flash red.
When the temperature of the heating platen reaches the set temperature, the display will show Ready, and the indicator LED will be a
steady green.
NOTE:
It is recommended to leave the drawer closed, and without a sample plate on the drawer while the platen is heating up. This
will allow the unit to heat to the set temperature more quickly.
3.2 Set the Time
Pressing the Mode button until the Seal Time text is flashing. Use the control knob to adjust the display to the desired sealing time.
Press the control knob or wait 10 seconds to set the selected time.
3.3 Using Plate Adapters
Labnet ships adapters for optimal sealing of certain multiwell plate configurations.
Labnet AccuSeal adapter for sealing assay and PCR
microplates
One side (96 holes) accommodates 96-well microplates
and the other side accommodates 384-well PCR
microplates.
Labnet AccuSeal support block for assay and
storage plates
A thin block that is designed to support the center
portion of assay and storage plates.
96-well microplate side
384-well microplate side
Support block
3.4 Using the Seal Frame
A sealing frame is provided with the unit to assist in securing the thermal seal in proper position prior to sealing the plate. This
ensures proper positioning of the seal and prevents any curling or undesired movement of the thermal seal prior to the sealing
process.
After loading the plate and placing the seal on top of the plate, the frame is then lowered over the thermal seal and onto the plate.
Tabs at either end of the frame secure the thermal seal in place. There are 4 metal posts around the plate which serve as indexing
points for the frame.
1. Rubber handles:
Provides insulated handling points for the frame.
2. Tabs:
Secures the thermal seal in place.
3. Indexing points:
Fits around metal posts on the plate drawer.
