Montgomery County Community College
Document Number: UP 1
340 DeKalb Pike
Revision Number: 0
Blue Bell, PA 19446
Effective Date: 18JAN14
Page 1 of 4
SOP: Labconco Purifier Class 2 Biological Safety Cabinet (BSC) Operation
Approvals:
Preparer: Jason McMillan
Date: 17JAN14
Reviewer: Dr. Margaret Bryans
Date: 18JAN14
1.
Purpose:
1.1.
Operation of the Biological Safety Cabinet (BSC).
2.
Scope:
2.1.
Applies to the use of the BSC for maintaining a sterile environment for media
preparation, culture inoculation and culture sampling.
3.
Responsibilities:
3.1.
It is the responsibility of the course instructor/lab assistant to ensure that this SOP is
performed as described and to update the procedure when necessary.
3.2.
It is the responsibility of the students/technicians to follow the SOP as described and to
inform the instructor about any deviations or problems that may occur while performing
the procedure.
4.
References:
4.1.
Labconco Purifier Class 2 Biological Safety Cabinet Operator’s Manual
5.
Definitions:
N/A
6.
Precautions:
6.1.
UV Light is damaging to eyes and skin. Avoid exposure.
7.
Materials:
7.1.
70% Isopropanol (IPA) in spray bottle
7.2.
lab towels
7.3.
lab coat
7.4.
gloves
8.
Procedure:
8.1.
UV Decontamination – Performed for initial use of the day
8.1.1.
Put on gloves and lab coat.
8.1.2.
Open sash
slightly
and
immediately
turn on blower.
8.1.3.
Spray and wipe down the stainless steel work surfaces of the BSC with 70% IPA.
8.1.4.
With gloved hands, spray all necessary materials not affected by UV with 70%
IPA and place in BSC.
8.1.5.
Close the sash, turn off the blower, and turn on the UV light. This switch is
located on the control panel. Refer to Figure 1.
8.1.6.
Leave the UV light on for at least 15 minutes.
8.1.7.
Place a biohazard waste receptacle adjacent to the cabinet.
8.1.8.
Once the appropriate time has elapsed, turn off the UV light.
8.1.9.
Turn on the visible light. This is located on the control panel.
8.1.10.
Turn on the receptacle power.
8.1.11.
Open the sash
slightly,
and
immediately
turn on the blower.
8.1.12.
After blower is on, raise the sash to the safe operating level indicated by a red dot
on the left side of the cabinet.
