KNAUER
P 6.1L User Manual V6890 Version 1.2
64
HPLC Glossary
HPLC Glossary
In the following chapter you find abbreviations and terminology
that is used in HPLC.
Term
Definition
Binary
pump
Pump configured to generate a gradient from
two solvents.
Capillary
Thin metal or PEEK pipe that connects the
components and devices inside of an HPLC sys-
tem.
Check valve
Valve unit built into the pump head which
guides the flow of liquid from the inlet to the
outlet.
Chromato-
gram
The record of a detector signal, depending on
the flow volume of the mobile phase or time.
Column
The column is filled with a matrix which sepa-
rates the single components of a substance
spatially by interacting with an analyte trans-
ported with the solvent to different degrees.
Control
Unit
Hand-held device with a touchscreen to drive
single AZURA devices locally.
Dead vol-
ume
Volume of capillary and system components
between mixer, injector and column as well as
between column and detector.
Degasser
Degasser module for fluids
Detector
Device measuring the composition or the
quantity of a substance.
Eluent
Flowing agent that transports the substances
to be separated or isolated through the column
(solvent, mobile phase)
Flangeless
fitting
Fitting without a protruding edge, which can
be fastened manually.
GLP
Quality assurance system for laboratories
(Good Laboratory Practice)
Gradient
Time-dependent composition of solvent
(mobile phase) on low-pressure or high-pres-
sure side of system
Ground
Protective measure for electro-conductive
housing parts
HPG pump
Pump configured to generate a gradient on the
high-pressure side. Usually a binary pump with
two separate pump units which each convey
one solvent. The gradient is generated behind
the pump.
