Knauer Azura ECD 2.1, Instructions Manual

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24 Maintenance & shutdown
AZURA® Detector ECD 2.1 Instructions, V6685
of salt crusts (in case of buffered mobile phases), which could block the
drain in the bottom of the cell cabinet. Remove any dust on the protective
screens that cover the fans in the oven compartment.
Replacement of fuses
Replace blown fuses with fuses of proper type and rating as stipulated
on the rear panel and specified in the installation section of these instruc-
tions. The fuse holder is integrated in the mains connector. Ensure that
the instrument is never put in operation with fuses of a different type. This
could cause fire.
In case the fuses blow out repetitively contact KNAUER or its representati-
ves for instructions and/or service of the instrument.
4.2 Shutting down the system
ƒ
There are a couple of steps to take to switch off an LC system with
electrochemical detector for a longer period of time. Shutting down
is not different from most other HPLC systems. Perform the following
procedure:
ƒ
Switch off the flow cell using the keyboard (standalone) or via the soft-
ware (ClarityChrom® or Dialogue Elite).
ƒ
Check the column(s) documentation for the appropriate storage liquid,
apply this and make sure the column is properly flushed. A reversed
phase C18 column is usually stored with 50% Acetonitrile/water.
ƒ
Take out the column, mount the corresponding end-caps and store the
column in an appropriate place.
Avoid precipitation of high salt concentrations in organic solvent,
first wash out salts with water if necessary.
ƒ
Flush and store the system with 50% water/acetonitrile (or methanol).
Switch the injector valve between load and inject a few times. Make
sure all tubing, filters etc. are flushed so no traces of salt are left that
could precipitate and clog the system.
ƒ
Remove the flow cell from the system by disconnecting the inlet and
outlet capillary.