Pump P 2.1L
Instructions
Document no. V6840
HPLC
Page 1: ...Pump P 2 1L Instructions Document no V6840 HPLC HPLC...
Page 2: ...auer net KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 Internet www knauer net E Mail info knauer net Document number V6840 Version nu...
Page 3: ...s 3 2 1 2 Foreseeable misuse 3 2 2 User qualification 3 2 3 Operator responsibility 4 2 4 Personal safety equipment 4 2 5 Safety features on the device 4 2 6 Working with solvents 5 2 6 1 General requ...
Page 4: ...up to LPG 19 4 6 1 Mounting the valve block 20 4 6 2 Connecting the eluent line to the pump head 21 4 7 Priming the pump 22 4 8 Leak management 23 4 9 Control 24 4 9 1 Setting up LAN 24 4 9 2 Configur...
Page 5: ...ation Qualification IQ 43 6 1 1 Definition of the installation qualification 43 6 1 2 Scope 43 6 1 3 Instructions 43 6 1 4 About this document 43 6 1 5 Device data 44 6 1 6 Customer data 44 6 1 7 Pack...
Page 6: ...2 Packing the device 59 9 3 Transporting the device 59 9 4 Storing the device 59 9 4 1 Storing the pump head 60 10 Disposal 61 10 1 AVV Marking Germany 61 10 2 WEEE registration number 61 10 3 Eluents...
Page 7: ...imes You can download these and other instructions from the KNAUER web site www knauer net library 1 2 Signal words Possible dangers related to the device are distinguished in personal and material da...
Page 8: ...ng company within three workdays 1 4 3 Warranty conditions For information on warranty please refer to our general terms and condi tions on the website www knauer net terms 1 4 4 Warranty seal A blue...
Page 9: ...y of the device Operation in potentially explosive areas without special and additio nal explosion protection Contact the KNAUER Customer Support for more information 2 2 User qualification The users...
Page 10: ...to the employees working with the unit see section below 2 4 Personal safety equipment The protective measures required in the laboratory must be observed and the following protective clothing worn du...
Page 11: ...s could get onto hot surfaces in the interior Degas solvents before use and observe their purity 2 6 2 Contamination by health threatening solvents Contamination with toxic infectious or radioactive s...
Page 12: ...n wet rooms 2 8 Maintenance care and repair Avoiding electric shock Before performing any maintenance and ser vice work disconnect the device from the power supply Tools Use only tools recommended or...
Page 13: ...st form and decontamination report Devices which are shipped without the completed document Service request form and decontamination report will not be repaired If you would like to return a device to...
Page 14: ...ush ing Leak management KNAUER offers the following accessories Stainless steel pump heads Titanium pump heads for biocompatible applications Display software Mobile Control Heating cooling elements f...
Page 15: ...ig 1 1 2 3 Pump P 2 1L front view Legend 1 Left LED 2 Center LED 3 Right LED 4 Standby switch Fig 2 1 2 3 4 LEDs and switch on the front of the device Legend 1 Serial number 2 Pin header 3 LAN port 4...
Page 16: ...aration of fine chemicals Separation of active pharmaceutical ingredients API 3 4 Pump heads The pump automatically recognizes the pump head by means of the RFID chips The pump head is equipped with a...
Page 17: ...d temperature range 2 Not recommended in combination with PEEK small parts and PEEK capillary Less suitable solvents Carbon dioxide liquid 99 999 CO2 Dilute phosphoriac acid Dimethyl sulfoxide DMSO Me...
Page 18: ...Shortly press the switch to deac tivate the error message Red Serious error Restart the device If the operating condition does not change con tact the Technical Support Green Program or sequen ce is r...
Page 19: ...s and signs The following symbols and signs can be found on the device Symbol Meaning High voltage hazard Electrostatic discharge hazard damages to sys tem device or components can occur Obey maximum...
Page 20: ...ched to the device Keep the included packing list for repeat orders Keep the original packaging for safe storage or transportation 4 2 Ambient conditions 4 2 1 Operation site Observe the following req...
Page 21: ...connected devices must not exceed 50 of the highest connected power capacity since higher currents can flow briefly when the device is switched on Main connection The electrical power supply at the o...
Page 22: ...lush pump are located on the front of the device The flush pump is inside of the device and not visible from the outside Fig 5 Inlet symbol Fig 6 Outlet symbol The pump has been set up at the site of...
Page 23: ...nd pressures up to 400 bar for 1 16 and 200 bar for 1 8 4 5 Eluent inlet The eluent lines are connected by the eluent inlet to the pump head Before the eluent lines are connected the eluent inlet must...
Page 24: ...ml make sure that the tapered side of the cutting ring is pointed towards the fastening screw of the Teflon hose The eluent inlet has been connected Make sure that all connections are tight Make sure...
Page 25: ...valve block the setup of the system is changed to low pressure gradient LPG The valve block is mounted to the front of the pump There are two types of valve blocks Ternary valve block for flow rates f...
Page 26: ...s place seals in pairs on both fastening screws Process Figure Procedure 1 Unscrew the inlet 1 from the pump head Fig 14 1 Inlet on the pump head 2 Place the seal rings 3 on the screws and valve block...
Page 27: ...flon hose 1 through the fastening screw 2 and the cutting ring 3 2 Insert the hose end as far as possible into the eluent inlet fit ting 4 of the pump head Fig 17 Cutting ring with Teflon hose 3 Tight...
Page 28: ...1 Open the venting screw 1of the pressure sensor 2 With the syringe extract fluid through the priming port 2 3 If the extracted fluid flows con tinuously stop suction and close the venting screw Fig 1...
Page 29: ...1 Carefully push the funnel 1 into the center opening of the capillary guide 2 Fig 20 1 2 Funnel and capillary guide 2 Push the long ending of the first nozzle 4 into the hose 3 Fig 21 3 4 Hose and n...
Page 30: ...addresses are needed see section 4 10 Setting IP addresses via software on page 27 The following chapters describe how to set up a chromatography system in a local area network LAN and how a network...
Page 31: ...utton Properties 4 Select Internet Protocol version 4 TCP IPv4 5 Click on the button Properties 6 Check the settings in the tab General The correct settings for the DHCP client are a Obtain an IP addr...
Page 32: ...on about address user name and password is noted in the router manual www knauer net router Process 1 To open the router configuration start your Internet browser and enter the IP address does not app...
Page 33: ...e the same num bers in the device configuration of the chromatography software as in the device otherwise the connection fails Process 1 Find out port number and change it on the device 2 Enter the po...
Page 34: ...corner 7 Restart the device recommended 1 IP address mode 2 Text box for IP address 3 Text field for subnet mask gateway Fig 26 Network settings for static IP address The device is now accessible via...
Page 35: ...l is installed and running The connection between Mobile Control and the device has been established Process 1 In Mobile Control click Settings 2 In Network Settings click Reset The windows Reset comm...
Page 36: ...d on Firmware Wizard is installed and running Process 1 In Firmware Wizard click Reset LAN Settings 2 The window Device connection settings opens Enter serial num ber of the device into the text field...
Page 37: ...e window Device connection settings opens Enter serial number of the device into the text field Target device serial number 1 3 Select option Obtain an IP address automatically 2 4 Click Reset Conn Se...
Page 38: ...range can be set to values of max 1 2 5 and 10 V Analog IN Analog input signal for controlling the flow rate e g 1 V for 10 ml min in the case of the 100 ml pump head 1 V for 25 ml min in the case of...
Page 39: ...the signal inputs Events remote connector For test purposes or in some other cases it can make sense to manually enter these signals Sending control signals Events to external devices Opening and clos...
Page 40: ...t least 1000 ms 5TTL TTL compatible output Levels passive 0 V active 5 V Pulse 5 V for at least 1000 ms 6OC TTL output Levels passive 0 V active 5 V Pulse 5 V for at least 1000 ms GND Reference point...
Page 41: ...nge 0 10 V 10 V according to maximum flow rate Start GND Reference point of the voltage at the signal inputs Start OUT TTL output Levels passive 5 V active 0 V Start IN TTL input Low active Secure swi...
Page 42: ...on device causes a short circuit Turn off the device before connecting cables Pull the power plug Electronic defect Electrostatic discharge can destroy the electronics Wear a protective bracelet agai...
Page 43: ...12 Analog control Note Contact the customer support to change the default settings and to select the analog control Analog ports serve for exchanging analog control signals Reference point for the sig...
Page 44: ...the correct solvent as described in the specification table found in the supplement Running In procedure for pump heads V6894 If a pump was not in operation for a long time e g after shipment a runnin...
Page 45: ...thin shortly The installation has been completed The capillaries and hoses have been connected The pump has been switched on and is in flow mode Syringe with Luer lock Use the purging solvent to be us...
Page 46: ...a If this value is exceeded during the purging process the pump switches off automatically If you are using very small hoses and capilla ries the pressure can be too high If there are air bubbles in t...
Page 47: ...simultaneously If the device is connected to the software it cannot be controlled via Mobile Control 5 4 1 Controlling with chromatography software To operate the device with software you have to esta...
Page 48: ...been purged see 5 2 Purging the pump on p 39 Use isopropanol if you are going to take the device out of operation for a longer period of time or if you want to prepare it for storage The device is no...
Page 49: ...action undertaken to resolve the problems and remove the deviations shall be recorded as comments in the section 6 1 9 Com ments recommendations on page 45 If certain items in the report are not appl...
Page 50: ...g 38 14163 Berlin Phone 49 30 809727 111 E Mail support knauer net KNAUER agent 6 1 6 Customer data Customer Customer no Company Contact person Address Postal code City Phone E Mail Location Place of...
Page 51: ...ice to the power supply The device is connected 5 Connect all loose parts e g capil laries tubings measuring head The device is fully assembled and ready to use 6 Switch on the device The device start...
Page 52: ...ality test accor ding to KNAUER standard OQ documents The Operation Qualification is a standardized document and free of charge It is not part of the delivery Please contact the Technical Support in c...
Page 53: ...he router set to DCHP server Is the IP address range sufficient for all the connected devices 3 Check all connections Are the patch cables connected to the LAN ports and not the WAN port Are all cable...
Page 54: ...the manufacturer Exchange the pump head Pressure and flow rate variations Purge the pump head to remove the air bub bles Let the pump run for 1 h Always tighten the inlet screw fittings and outlet scr...
Page 55: ...n Auto pump head type RFID hard ware not pre sent or failed Pump head without RFID detection If necessary replace pump head Auto pump head type read failed Switch the device off and on Repeat the auto...
Page 56: ...se the sys tem message repeats itself Component set tings not compati ble with gradient setup Change the setup settings or change the gradi ent in the program or in setup E Error input acti vated Devi...
Page 57: ...etup No link available Create a link and edit it No link available Pls edit link first Create a link and edit it No time table to start Edit the data by means of the chromato graphy software Non exist...
Page 58: ...of program lines A maximum of 100 program lines are possible U Unable to attain min flow set point Confirm pump continues running Unknown pump head type Check the pump head Check whether a pump head w...
Page 59: ...emselves Replacing the pump head Replacing the check valves of the pumps Proper maintenance of your HPLC device will ensure successful analyses and reproducible results 8 1 Maintenance contract The fo...
Page 60: ...t Fig 35 2 3 1 4 5 Screw fittings on the pump head 8 3 2 Dismounting the pump head The pump head has been purged The tubes and hoses have been removed Allen wrench Open end wrench sizes 10 17 Chemical...
Page 61: ...s of the piston backflushing 2 from the flush pump and the pump head 3 Remove the eluent lines 4 from the eluent inlets 4 Unscrew the Allen screws 3 5 Hold the pump head by hand and consecutively pull...
Page 62: ...r 1 8 Process Figure Procedure 1 Slide the fitting 1 onto the capillary 2 2 Slide the clamping ring 3 onto the capillary so the capillary comes out Fig 37 1 2 3 Screw fitting 3 Using an open end wrenc...
Page 63: ...1 Removing the check valves The pump head has been purged The capillaries have been removed The pump head has been dismounted Open end wrench sizes 10 17 Process Figure 1 Unscrew the outlet fittings...
Page 64: ...ll and position of the valves have been harmonized to each other Insert the valves in the direction of flow The pump head has been purged The capillaries have been removed The pump head has been dismo...
Page 65: ...d onto front cover or leak tray as these parts are loosely attached to the device 9 3 Transporting the device Documents If you want to return your device to KNAUER for repairs enclose the Service requ...
Page 66: ...pillary at the pump head inlet Leave it for 5 minutes 2 Flush with suitable flushing solution 3 Fill the pump head with isopropanol 4 Seal the inlets and outles with cap fittings The pump head can be...
Page 67: ...lassifies to category 8 and 9 which among others comprises laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End user...
Page 68: ...continous working 1000 ml pump head 1 1000 ml min 10 800 ml min recommended 10 400 ml min continous working Maximum pressure 100 ml pump head 400 bar to 100 ml min 250 ml pump head 225 bar to 100 ml m...
Page 69: ...Pump maximum 320 W Binary or ternary valve block 5 W Protection type IP 20 11 3 General Power Supply Pump 100 240 V 50 60 Hz Binary valve block 24 V Ternary valve block 12 V Dimensions 361 mm x 208 m...
Page 70: ...wise Please note that higher temperatures can signifi cantly affect the stability of different materials 12 2 Plastics Polyetheretherketone PEEK PEEK is a durable and resistant plastic and next to sta...
Page 71: ...e POM POM HTF POM is a semi crystalline high molecular thermoplastic material which stands out due to its high stiffness low friction value and thermic stability It can even substitute metal in many c...
Page 72: ...ized by a high hardness a low coefficient of fric tion and thus low wear In addition it is highly biocompatible DLC is inert against all acids alkalis and solvents commonly used in HPLC Ceramic Cerami...
Page 73: ...sed or with a mixture of higher compatibility They are inert against almost all solvents Exceptions are biological appli cations which are metal ion sensible and applications with extreme cor rosive c...
Page 74: ...um APE20KB Pump P 2 1L with 250 ml pump head stainless steel APE20LA Pump P 2 1L with 250 ml pump head titanium APE20LC Pump P 2 1L with 500 ml pump head stainless steel APE20MA Pump P 2 1L with 500 m...
Page 75: ...ium A4038V2 Pump head 1000 ml stainless steel A4022 1 Pump head 1000 ml titanium A4022V2 Check valves Check valve unit for 100 ml and 250 ml pump heads A1122 Check valve unit for 500 ml and 1000 ml pu...
Page 76: ...UER instructions online www knauer net library KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Phone Fax E Mail Internet 49 30 809727 0 49 30 8015010 info knauer net www knauer net KN...
