Figure 5
Adipocyte differentiation properties of KM-transduced 3T3-L1 preadipocytes and MSCs derived from bone marrow (KUSA-A1, KUM5 and KUM9 cells). (
a
) Phase
contrast microscope images. 3T3-L1 preadipocytes were transduced with KM Sendai virus. One day after infection, cells were cultured in reprogramming medium for 8 days.
At day 8, cells were fixed and stained with Oil Red O. The white bar indicates 50
μ
m. Cntrl indicates untreated 3T3-L1 preadipocytes. (
b
) qRT-PCR analysis of the expression of
each gene in 3T3-L1 preadipocytes on each day. Individual RNA expression levels were normalized to
Gapdh
expression. Error bars indicate S.E. (
n
=
3). * and ** indicate
significant changes compared with KM-treated 3T3-L1 cells at day 8 (
P
o
0.05 and 0.01, respectively). (
c
) Phase contrast microscope images. MSCs derived from mouse bone
marrow were transduced with KM Sendai virus. One day after infection, cells were cultured in reprogramming medium for 8 days. At day 8, cells were fixed and stained with Oil
Red O. The white bar indicates 50
μ
m. Cntrl indicates untreated MSCs. (
d
) qRT-PCR analysis of the expression of each gene in MSCs KUSA-A1 (black bars), KUM5 (gray bars)
and KUM9 (white bars) at day 8. Individual RNA expression levels were normalized to
Gapdh
expression. Error bars indicate S.E. (
n
=
3). * and ** indicate significant changes
from untreated control cells (KM
–
, day
–
2) (
P
o
0.05 and 0.01, respectively)
Cardiac adiposity is regulated by
Klf4
and
c-Myc
D Kami
et al
7
Cell Death and Disease
