Operating Instructions
HERAcell
®
240 CO
2
Incubator
with Decontamination Routine
Version: 03.2001 / 50065640
Page 1: ...Operating Instructions HERAcell 240 CO2 Incubator with Decontamination Routine Version 03 2001 50065640...
Page 2: ...HERAcell 240 CO2 Incubator Operating Instructions 2 Version 03 2001 50065640...
Page 3: ...er Atmosphere 21 5 2 ContraCon Decontamination Routine 21 5 3 Sensors 22 5 4 Door Interlock Switch 22 5 5 Supply Connections 23 5 6 Sample Chamber Layout 24 6 Initial Setup 26 6 1 Sample Chamber Prepa...
Page 4: ...54 11 1 Inspections and Checks 54 11 2 Maintenance Intervals 54 11 3 Checking the Temperature 55 11 4 Equalizing the Temperature 56 11 5 Checking the CO2 Concentration 57 11 6 Equalizing the CO2 Conc...
Page 5: ...Support profile installation removal 26 Fig 14 Support bracket insert tray installation 27 Fig 15 Center support installation 28 Fig 16 Gas supply line connection 29 Fig 17 Power connection 30 Fig 18...
Page 6: ...be provided in the lan guage of the employees Using these instructions instruct the operating and cleaning personnel in the functions the ope ration and the required cleaning tasks for this unit The c...
Page 7: ...ducts GmbH Any other trade marks cited in these operating instructions are the sole property of the associated manufacturers Copyright These operating instructions are copyrighted Reprin ting photomec...
Page 8: ...serious injury or property damage NOTE Indicates important information and application tips RECYCLING Valuable raw materials can be recovered Wear protective gloves Wear safety glasses Hazardous liqui...
Page 9: ...the operating instructions VDE tested safety nameplate 1 Information regarding Safe Operation Symbols in the Short Instructions Check humidifying system water level and top up as required Max fill vol...
Page 10: ...humi dity It is equipped with an automatic decontamination rou tine The HERAcell 240 CO2 Incubator is intended to pro vide an environment with controlled temperature CO2 elevated humidity and automati...
Page 11: ...ding this fee based service please contact the Kendro Service 1 Information regarding Safe Operation Safety Regulations The following safety regulations apply to the operation of the unit in the Feder...
Page 12: ...t damage and deficiencies in the CO2 supply lines Measures to be instituted in case of accidents and faults Instruction is to be repeated at suitable intervals All special instructions provided by the...
Page 13: ...complete Should damage be discovered or if components are missing immediately inform the carrier Kendro Labo ratory Products or your dealer 2 Initial Inspection 2 1 Packaging The HERAcell 240 CO2 Inc...
Page 14: ...s diaphragm with continuous inserts Insert tray 3 6 6 3 Insert tray support pro files 4 6 6 4 Insert tray support bracket 6 12 12 6 Drawer 1 1 1 1 Pressure compensation opening insert 1 1 1 1 Access p...
Page 15: ...tilation system should conform to the requirements of ZH 1 119 laboratory guideli nes 3 1 Ambient Conditions The unit may only be operated at setup locations which conform to the special ambient condi...
Page 16: ...to adjacent objects The dimensions provided regarding space and clea rances required are to be considered the minimum re quirements NOTE Unit Accessibility We recommend maintaining larger than spe ci...
Page 17: ...it and the top of the adapter panel The unit feet 1 on the underside of the unit and the underside of the adapter panel mate with these elements to ensure a secure hold for the top unit When stacking...
Page 18: ...he glass door NOTE Modifications Door modifications may only be performed by the Kendro Laboratory Products Service 3 7 Drawer Once the unit has been set up the drawer is inserted between the two guid...
Page 19: ...opening 7 Exterior door 8 Exterior door seal replaceable 9 Foot for height adjustment 10 Drawer pull out 4 Parts Identification Fig 5 Front view Figure 5 11 Nameplate 12 Main power switch 13 Support p...
Page 20: ...03 2001 50065640 4 Parts Identification 4 2 Rear View 1 Circuit cabinet with supply connections Gas connection RS 232 interface Zero potential alarm contact Mains power connection 2 Access port 42 mm...
Page 21: ...auto start routine 5 System Description 5 1 Sample Chamber Atmosphere The special physiological conditions for the preparati on and cultivation of cell and tissue cultures are si mulated in the incub...
Page 22: ...selected setpoint temperature is exceeded by more than 1 C the overtemperature protector is activated and the sample chamber tempe rature is automatically returned to the defined set point Therefore...
Page 23: ...particle size of 0 3 m HEPA filter quality Nameplate The nameplate 3 contains information concerning the supply connections RS 232 Interface The RS 232 interface 4 can be used to connect the incubator...
Page 24: ...copper parts The effects of heat and humidity will oxidize the copper material in the interior chamber Thus the copper parts will already begin to discolor during the test run that is part of the init...
Page 25: ...t s re ar wall equalizes the pressure between the sample chamber and the ambient environment An access port 1 which can be sealed when not in use allows lines tubes or additional sensors to be added t...
Page 26: ...the rack system The support profiles are held in place by spring pressure The support brackets are hung into the support pro files and the insert trays slide onto the support brak kets Support Profile...
Page 27: ...ays 4 with their tip protection 5 towards the rear unit wall onto the support brak kets The tip protection 5 simultaneously serves as the guide for the insert tray 2 Slightly raise the insert tray so...
Page 28: ...ackets are inserted in the side support profiles and the perforations on the left and right sides of the center supports 1 The upper rectangular guide 3 of the center sup port is under spring force Fi...
Page 29: ...res sure cannot be altered The gas supply is connected to the gas connection piece on the sterile filter using the included flexible gas pressure hose which is secured by a hose clamp 1 Push the gas p...
Page 30: ...er supply with the following connection values Fuse T 16 A Power overload cut out G 16 Establishing the mains power connection 1 Before connecting the unit to the mains power supply make sure the supp...
Page 31: ...ort 1 on the CO2 incubator s circuit cabinet on the rear of the unit 3 Insert the plug 3 at the other end of the cable into an open serial port on the PC COM 1 COM 2 etc 4 Switch the PC on Transfer Pr...
Page 32: ...displays e g error code 99 The error codes for incubation temperature and de contamination temperature appear on the temperature display while those for CO2 appear on the CO2 dis play A value of 0 in...
Page 33: ...only warrants the safety and proper operability if the installation and re pair tasks are performed professionally Only an authorized electrotechnical telecommunica tions specialist may connect the un...
Page 34: ...larm Relay Note Circuit structure The alarm relay is activated by all errors re ported by the unit probe break setpoint exceeded not reached and door open longer than 10 minutes Operating mode Contact...
Page 35: ...ands sensors in a single automatic pro gram run 1 Check that the insert trays in the sample chamber are empty and that no tools or materials have be en left in the unit 2 Add 350 ml of treated water...
Page 36: ...s divided into three functional areas 2 displays to indicate numeric values for tempe rature and CO2 content 8 keys to call up functions and input data 7 LED indicators showing a function or state 1 T...
Page 37: ...Operator action Display explanation 1 Switch the unit on Press the main power switch All indicators on the control panel go on The software version designation is shown on the temperature display and...
Page 38: ...C keys Increase the setpoint Decrease the setpoint 3 Save setpoint Release the C key The current actual value in the sample cham ber is shown on the temperature display 7 5 CO2 Setpoint Specification...
Page 39: ...Change the mode Press the key or Press the key The new mode Low humidity is shown on the temperature display Example 4 Save the target mode Press the key The actual values are shown on the tempe ratu...
Page 40: ...east quarterly as part of normal cleaning and maintenance work As a rule the routine requires 5 to 7 hours Where the ambient temperature is low or the unit is cold the routine may take up to 10 hours...
Page 41: ...question together with your unit s ID no ready NOTE Activation delay In order to prevent brief fluctuations in the operating conditions from leading to error codes being constantly generated during th...
Page 42: ...ror is reco gnized Example 4 View the error code Press the key The error code appears on the temperature display The CO2 display remains blank Example 5 Call up the error table Press the keys The most...
Page 43: ...ro Service 101 Temperature above set point Actual value setpoint 1 C Make sure ambient temperature is below permissible maximum Contact the Kendro Service 104 Temperature probe faulty Probe break shor...
Page 44: ...witch the unit on again Press the main power switch All displays on the control panel go on NOTE Overtemperature protection Once the cause of the problem has been corrected e g ambient temperature too...
Page 45: ...ovide adequate air circulation and uni form heating of the samples the available area in the sample chamber should only be filled to approx 70 Objects with large surface areas or equipment which gener...
Page 46: ...r 10 3 Add 350 ml of fresh treated water and start the ContraCon decontamination routine Once the ContraCon decontamination routine has be en completed disconnect the unit from the power and gas suppl...
Page 47: ...g and disinfecting the unit Wear protective gloves Wear safety glasses Wear a mask that covers your nose and mouth to protect their mucus membranes Observe all requirements of the disinfecting agent m...
Page 48: ...ed if required 1 Using the provided Allen wrench 3 mm remove the two fastening screws 3 on the protective screen then remove the screen 2 The fan wheel 1 is secured to the shaft by the stud screw 2 Lo...
Page 49: ...hwashing liquid 2 Use a soft clean cloth to wipe the surfaces dry 10 3 Manual Disinfection The unit can be disinfected by hand using a disinfec ting rinse spray The rack system components as well as t...
Page 50: ...sinfecting Agents Kendro Laboratory Products recommends using Bar rycidal 36 for disinfection Properly employed Barrycidal 36 represents a highly effective broad band disinfecting agent Its effect is...
Page 51: ...e routine requires 5 to 7 hours Where the ambient temperature is low or the unit is cold the routine may take up to 10 hours 10 Cleaning and Disinfection 10 4 ContraCon Decontamination Routine The Con...
Page 52: ...of the decontamination routine process The ContraCon Decontamination Routine The routine is subdivided into four phases Each of these phases can be interrupted individually and thus be skipped Initiat...
Page 53: ...30 sec 3 Start the ContraCon routine Press the key for 5 sec The ContraCon Routine LED flashes 4 Close both unit doors The actual value appears on the temperature display The CO2 display indicates the...
Page 54: ...co vering all required inspection and maintenance tasks 11 1 Inspections and Checks In order to maintain the operational efficiency and safety of the unit we recommend performing an in spection of th...
Page 55: ...tainer A container filled with water must not be used as an isothermal container as the water s evaporation will cause the instrument to indicate a temperature that is below the actual temperature Per...
Page 56: ...l panel flash 2 Display the setpoint Press the C key Preset value of 37 C Example 3 Enter the measured value target value Press the key or Press the key Target value e g 36 4 C Example 4 Save the targ...
Page 57: ...and Accessories for the order number The sample is taken via the measurement opening in the glass door The comparative measurement must be made when the unit temperature has stabilized Performing a C...
Page 58: ...5 Example 3 Enter the measured value target value Press the key or Press the key Target value e g 5 6 Example 4 Save the target value Press the key CAL will appear briefly on the CO2 display After th...
Page 59: ...as hose 4 off the connecting piece on the sterile filter 2 4 Unscrew the retaining panel 1 5 Unscrew the sterile filter 2 from its threaded sok ket 3 6 Taking care not to cross thread the new sterile...
Page 60: ...with a new one 4 Slide the fuse holder back into the fuse box and gently press down on it until the clamp tabs lock in place 11 9 Door Seal Replacement The door seal magnetic seal on the outer door re...
Page 61: ...Stainless steel 50067226 Divided inserts retrofit kit Copper 50067227 Replacement cover panels for front hinges Set 50052958 Magnetic door seal exterior door 778 x 858 50063335 Glass door seal 3 m 50...
Page 62: ...lug for access port 50063283 IR C02 gas tester hand held unit incl charger 100 V 230 V 50060283 Replacement filters 5 for IR CO2 gas tester 50060287 IrDa computer interface with connecting cable incl...
Page 63: ...emperature ContraCon routine C 90 Temperature deviation spatial DIN 12880 part 2 At 37 C C 0 5 auto start routine duration to 37 C Ambient temperature 20 C hr 5 10 Temperature recovery time at 37 C wi...
Page 64: ...AC Nominal frequency Hz 50 60 Interference suppression DIN VDE 0875 Interference level N Protection method DIN 40 050 IP 20 Protection class l Overvoltage category IEC 1010 EN 61010 ll Contamination d...
Page 65: ...Inner door panel Galvanized sheet steel painted Operator and display panels Polyethylene Magnetic door seal Magnetic core covered in EMPP Heaters Silicon jacketed resistance heater coil Inner chamber...
Page 66: ...viruses and subviral agents with a potential hazard risk FRG G 43 Biotechnology Information concerning first aid in cases of acci dental exposure to pathogenic microorganisms and viruses must be immed...
Page 67: ...undheitsdienst und Wohl fahrtspflege as well as on request from the testing department of the professional committee Health Services and Welfare The committee can be re ached at the BG for Gesundheits...
Page 68: ...01 50065640 NOTE Unit log Enter the nameplate information and any work maintenance and repairs carried out here Unit type Order number Manufacturer s number Service number Setup location Operator s co...
Page 69: ...HERAcell 240 CO2 Incubator Operating Instructions Version 03 2001 50065640 69...
Page 70: ...ons Kendro Laboratory Products Unit 4 2 6 Orion Road Lane Cove Sydney NSW 2066 Tel 61 02 9936 1540 Fax 61 02 9427 9765 info kendro com au China Kendro Laboratory Products Beijing Representative Office...
Page 71: ...81 0 3 3517 1664 info kendro com Austria Kendro Laboratory Products GmbH Wiegelestra e 4 A 1230 Vienna Tel 43 1 801 40 0 Fax 43 1 801 40 40 office kendro at Sweden Kendro Laboratory Products AB Hammar...
Page 72: ...HERAcell 240 CO2 Incubator Operating Instructions 72 Version 03 2001 50065640...
