iota Sciences isoHub, User Manual

Page: 19 / 33

18
5.4 Prep Extract
The
Prep Extract
program (
Prep Harvest
→
Prep Extract
) is used to select chambers to be removed
from the dish during the
Extract
program on the isoCell.
It can be run following a run of the
Detach
program on the isoCell for adherent cells, or alone for
suspension cells.
5.4.1 Adhesion cells
If the dish contains chambers that have had dissociation reagent added to them, then the isoHub
will move to all
detached chambers during the Prep Extract program. Detached chambers will have
been pre-selected to be extracted, but the user unselect chambers with colonies that aren’t ready
to be extracted. These colonies will then need to be reselected when they are ready to extract.
The LED illumination will be white when viewing chambers that have been marked as being ready
for extraction, and blue for chambers that have been marked as not being ready for extraction.
5.4.2 Suspension cells
If the dish doesn't contain any chambers that have had dissociation reagent added to them, then
the isoHub will move to all filled clonal chambers during the
Prep Extract
program, and users can
mark which colonies should be extracted.
The LED illumination will be white when viewing chambers not marked as being ready for
extraction, and green for chambers that have been marked as being ready for extraction.
5.5 Prep Redetach
The Prep Redetach ( Prep Harvest
→
Prep Redetach ) program is used in the case where the Extract
program on the isoCell has failed to remove one or more colonies from the dish.
The user must select harvested chambers for re-detaching - the user can only select from harvested
chambers produced by the most recent run of the
Extract
program.
The LED illumination will be white when viewing chambers not marked for re-detaching, and green
for chambers that have been marked for re-detaching.
6 Other programs
6.1 Data
The
data
program (
more
→
data
) enables the user to:
•
Add or remove usernames.
• Erase individual dishes under a chosen username.