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Invitrogen iPrep, User Manual

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Invitrogen iPrep User Manual Download Page 29

 

13

Methods 

General Guidelines 

 

 

Introduction 

General guidelines for using the iPrep

™

 Purification Instrument and iPrep

™

 

Plastic disposables are discussed below.  

 

 

Recommended 
iPrep

™

 Kits 

The following iPrep

™

 Kits available from Invitrogen that are compatible for use 

with the iPrep

™

 Purification Instrument. Ordering information for the kits is on 

page xvi. 

Note:

 Do not use any other kits with the iPrep

™

 Instrument. 

 

iPrep

™

 Kit 

Sample Type 

Purification 

Protocol Time 

iPrep

™

 ChargeSwitch

®

 gDNA Tissue Kit 

Various animal tissues 

30 minutes 

iPrep

™

 ChargeSwitch

®

 Forensic Kit 

Various forensic samples including 
dried blood spots, hair follicles and 
hair shafts, cigarette butts 

20 minutes  

iPrep

™

 ChargeSwitch

®

 Buccal Cell Kit 

Human buccal cell swabs 

18 minutes 

iPrep

™

 GeneCatcher

™

 gDNA Blood Kit 

Human blood samples (fresh, whole 
blood, EDTA or citrate anti-
coagulated blood, frozen blood 
samples, and old, archived blood 
samples 

26 minutes 

 

Recommended 
iPrep

™

 Protocol 

Cards 

To use the iPrep

™

 Instrument, you need to purchase the iPrep

™

 Protocol Card 

separately from Invitrogen. Ordering information is on page xvi. The table 
below lists the recommended cards for each iPrep

™

 Kit. 

 

iPrep

™

 Kit 

Recommended iPrep

™

 Card 

iPrep

™

 ChargeSwitch

®

 gDNA Tissue Kit 

iPrep

™

 gDNA Blood and Tissue 

Card 

iPrep

™

 ChargeSwitch

®

 Forensic Kit 

iPrep

™

 Forensic Card (includes 

buccal protocol) 

iPrep

™

 ChargeSwitch

®

 Buccal Cell Kit 

iPrep

™

 Forensic Card (includes 

buccal protocol) 

iPrep

™

 GeneCatcher

™

 gDNA Blood Kit 

iPrep

™

 gDNA Blood and Tissue 

Card 

Continued on next page 

Summary of Contents for iPrep

Page 1: ...T 1 760 603 7200 F 1 760 602 6500 E tech service invitrogen com For country speci c contact information visit our web site at www invitrogen com iPrep Purification Instrument For automated purificati...

Page 2: ...ii...

Page 3: ...nstrument xiii Accessory Products xvi Introduction 1 Overview 1 Description of Parts 5 Experimental Overview 12 Methods 13 General Guidelines 13 Getting Started 15 Using the iPrep Purification Instrum...

Page 4: ...iv...

Page 5: ...ation see the iPrep Kit manual 1 Press Start to run a protocol Select the desired purification protocol 2 Open the iPrep door Remove the iPrep Cartridge Rack and iPrep Tip and Tube Rack to set up the...

Page 6: ...A as described in the iPrep Kit manual 14 Discard the used cartridges tips and tubes into biohazard waste Do not reuse the cartridges 15 To purify more samples using the same iPrep Card load the racks...

Page 7: ...Upon Receiving the Instrument Examine the unit carefully for any damage incurred during transit Any damage claims must be filed with the carrier The warranty does not cover in transit damage iPrep St...

Page 8: ...he symbols used on the iPrep Purification Instrument and in the manual are explained below High Temperature Caution Used near the heat block to indicate a warning resulting due to high temperature Bio...

Page 9: ...see purification kit manual for details Dispensing Volume 5 1000 l Dispensing Accuracy Blue Tips 5 20 l Less than 15 CV 21 200 l Less than 2 CV DN100N Tips 25 50 l Less than 5 CV 50 1000 l Less than 2...

Page 10: ...fication Instrument is subject to the conditions described in this manual The protection provided by the instrument may be impaired if the instrument is used in a manner not specified by Invitrogen Th...

Page 11: ...Remove the wrapped instrument box from the outer bottom cardboard Remove the aluminum sheet wrapping the instrument box 5 Remove the instrument from the inner cardboard box The instrument is now in a...

Page 12: ...p Door as described on page 6 to remove the platform stopper by removing the 3 screws using a small Philips head screwdriver 3 To remove the Styrofoam protector under the Syringe unit inside the iPrep...

Page 13: ...urification Instrument is shown below Rear and Side View of iPrep Instrument The rear and side view showing various parts of the iPrep Purification Instrument are shown below Rear View Side View Conti...

Page 14: ...o LEDs Power LED green indicates the power is ON and Alarm LED to indicate errors blinking red The Keypad to enter parameters and operate the instrument The keys described in the table below are for r...

Page 15: ...ain menu Test menu and Manual menu The first line shows the current level The second and third line show the executable commands on this level The fourth line describes the keys to use for executing t...

Page 16: ...r use with the iPrep Instrument are available separately from Invitrogen For more information visit www invitrogen com or contact Technical Support page 45 Product Quantity Catalog no iPrep Forensic C...

Page 17: ...iction enzyme digestion See page 5 for details on various parts of the system System Components The iPrep Purification system consists of iPrep Purification Instrument The iPrep Purification Instrumen...

Page 18: ...ontaining the purification reagents and the iPrep Tips and Tubes on the racks Place the assembled racks on the iPrep Platform 4 Select the purification parameters from the display to start the automat...

Page 19: ...r of magnetic beads into the purified DNA Built in safety features in the instrument enhance user safety The ChargeSwitch Technology The ChargeSwitch Technology CST is a novel magnetic bead based tech...

Page 20: ...solution Step 2 DNA Purification Any residual protein is digested using the protease and then washed away to leave pure intact DNA Step 3 Elution The pure DNA is then eluted into a small volume ready...

Page 21: ...for a front and rear view of the device The front view of an open iPrep Purification Instrument identifying various parts is shown below Syringe Unit The Syringe Unit contains 13 nozzles that simulta...

Page 22: ...ep Tip and Tube Rack allows easy placement of iPrep Tips Sample tubes with samples and Elution tubes to collect the eluate See page 8 for details User Interface The User Interface is located on the fr...

Page 23: ...rd Do not wipe or clean the card using volatile chemicals such as alcohol or equivalent Do not expose the card to water or any solution Note If you accidentally damaged the iPrep Protocol Card you can...

Page 24: ...ep Tip and Tube Rack The iPrep Tip and Tube Rack is a stainless steel rack designed to hold sample tubes tips in tip holders and elution tubes The rack dimensions are 11 3 inches l x 4 inches w x 3 in...

Page 25: ...reader provides a positive reading feedback which includes an audible beep and a green spot on the barcode Each iPrep instrument includes 1 barcode reader 1 CD ROM with CommViewer Barcode Software and...

Page 26: ...the protocol Cartridge Specifications Material Polypropylene cartridge sealed with laminated aluminum foil Max Volume 1000 l well Dimension 5 9 inches l x 1 2 inches w x 0 7 inches d iPrep Tips and Ho...

Page 27: ...ation protocol The iPrep sample and elution tubes are included with each iPrep Kit and placed in their respective positions on the iPrep Tip and Tube Rack as described on page 26 Tube Specifications M...

Page 28: ...s ChargeSwitch Magnetic beads are washed pH 7 0 Purified DNA is eluted from beads at pH 8 5 Materials Needed You will need the following items Ordering information is on page xvi iPrep Kits iPrep Prot...

Page 29: ...ette butts 20 minutes iPrep ChargeSwitch Buccal Cell Kit Human buccal cell swabs 18 minutes iPrep GeneCatcher gDNA Blood Kit Human blood samples fresh whole blood EDTA or citrate anti coagulated blood...

Page 30: ...ansfer the eluted sample to appropriate storage as described in the iPrep Kit manual Important You can load 1 13 iPrep Cartridges on the rack depending on the number of samples that you wish to proces...

Page 31: ...o the AC inlet and then to the electrical outlet Use only properly grounded AC outlets and power cords You are ready to use the iPrep Instrument for nucleic acid purification See page 24 for the detai...

Page 32: ...instrument ON Note To completely insert the card you need to manually push the card into the slot 5 Close the iPrep Card Slot 6 Using the Power Switch located on the left side of the instrument turn O...

Page 33: ...ate 2 Time Key 1 2 ESC 5 The Date Screen is displayed Setup Sys Date Cur CMN CD CYYY Set SMN SD SYYY Key Up Dn RET ESC Row Curr displays the current month on CMN current date on CD and current year on...

Page 34: ...esired date is displayed press Shift Down arrow to select the date and move the cursor to SYYY to display current year Note If the selected date exceeds the maximum date specified by SMN and SYYY then...

Page 35: ...ed hour Once the desired hour is displayed move the cursor to SM to display the current minutes using Shift Down arrow Note 0 is the lower limit and 23 is the upper limit for the hour settings Setup S...

Page 36: ...d below 1 Ensure the power switch is on the OFF position 2 Open the iPrep Card Slot 3 Push the button located below the card slot to eject the card from the slot 4 Pull out the card from the slot and...

Page 37: ...need to use a commercially available USB to serial converter cable Connect the USB end of the cable into the USB port on the computer and connect the serial end of the cable into the PC port of the iP...

Page 38: ...er InstallShield Wizard guides you through the installation process using the following steps Once the Welcome Window appears click Next This window allows you to select a folder to install the CommVi...

Page 39: ...igital display on iPrep screen prompts you to Press 1 to output data On pressing 1 the barcode data read using the barcode reader appears in the Receive Data window 7 Select the Clear Receive Data to...

Page 40: ...tion protocols are included in the manual supplied with the iPrep Kits or available for downloading from www invitrogen com Be sure the sample is prepared and ready for loading onto the instrument bef...

Page 41: ...lick if the cartridge is placed correctly on the rack Important Do not just place the cartridge on the rack You need to slide the cartridge into the rack and push down Do not load reagent cartridges f...

Page 42: ...in row marked as S Add the sample to each tube see the iPrep Kit manual for sample preparation details and sample volume recommendations The final assembly of iPrep Tip and Tube Rack is shown below 5...

Page 43: ...ument to a computer using a RS232C serial cable to the PC port on the rear of the iPrep Instrument page 21 Install the barcode reader software on the computer as described on page 22 3 Insert the appr...

Page 44: ...hrough the alphabet in case you wish to read letters The BS key is a backspace key that deletes the last digit character If you enter the sample number manually press Return to go to Screen 3 above Us...

Page 45: ...er for that sample on the iPrep keypad For example to read the first elution tube barcode press 1 on the keypad Elution 1 1 Elution 2 2 Elution 3 3 Rtn Next Esc Prev 3 Read the barcode on the elution...

Page 46: ...tus screens are displayed on the iPrep depending on whether the transmission was successful or rejected Successful Rejected Timeout Transmission Status Successful Press any key to continue Transmissio...

Page 47: ...fication protocol begins and various steps of the protocol including the approximate time remaining are displayed on the digital display Important Do not open the door once the protocol has begun To p...

Page 48: ...trical cord is accidentally removed from the outlet the purification protocol stops When the power resumes the digital display shows the main screen and the protocol cannot be continued An error is lo...

Page 49: ...ves all axes to the original position Press 1 to go to the ORG display to move all axes to the origin if the tips do not need to be returned to the holders Once in the ORG screen you can individually...

Page 50: ...t as described on page 16 2 Turn ON the instrument The Main screen is displayed 3 Press 3 to display the Test screen 4 The types of tests are displayed on the screen Axis test allows you to check move...

Page 51: ...rrectly press Start to begin the test The duration of the test is 3 minutes During the Axis test all well hole positions are checked by moving tips on each position The display indicates the status of...

Page 52: ...tically set to the preset value of 25 C To cool down the heater unit to this preset value turn off the instrument The temperature of the heater unit cools down at a rate of about 1 C per minute Do not...

Page 53: ...pe Performed by Schedule Routine Cleaning of platform surface racks and bottom tray Maintaining the D ring Replacing the D Ring User User User After each use Bi weekly Annually Preventive Service Engi...

Page 54: ...excess grease interferes with the operation of the instrument For any other repairs and service contact Technical Support page 45 Do not perform any repairs or service on the iPrep Instrument to avoi...

Page 55: ...displayed on the digital display Error code displayed Make a note of the error code and see the list of error codes on page 42 If your specific error code is listed on the list on page 42 you need to...

Page 56: ...ing the protocol To ensure proper mixing of reagents in the tip and prevent bubble formation during mixing make sure the sample volume is at least the recommended volume listed in the manual supplied...

Page 57: ...y damaged Make sure that the beads are in solution at all times and do not dry Dried beads are non functional No DNA recovered Accidentally missed adding tubes or tips Be sure to add the sample and el...

Page 58: ...ol in run 150 Invalid parentheses position 13 Failed to return to P axis protocol in run 151 Invalid big parentheses position 14 Failed to return to M axis protocol in run 160 Does not match variable...

Page 59: ...perature 119 Function pointer buffer overflowed 306 Protocol failed to key in 120 No of character of data read is overflowed 307 Protocol failed to input variable 121 No of character of one command in...

Page 60: ...chnical Support page 45 for a service engineer if the 6 3 A fuse needs replacement 1 Turn off the main power switch at the rear of the instrument and detach the power cord from the rear of the instrum...

Page 61: ...te fax or email Additional international offices are listed on our Web page www invitrogen com Corporate Headquarters Invitrogen Corporation 1600 Faraday Avenue Carlsbad CA 92008 USA Tel 1 760 603 720...

Page 62: ...not limited to 1 use of the product or its components in manufacturing 2 use of the product or its components to provide a service information or data 3 use of the product or its components for thera...

Page 63: ...by the Purchaser This Warranty and the remedies set forth herein are exclusive and in lieu of all other express or implied warranties including implied warranties of merchantability fitness for a part...

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